Repeatability and method-dependent variation of blood parameters in wild-caught Great Tits Parus major

• Autorzy: Norte A C , Sheldon B. , Sousa J.P. , Ramos J.A.

Warianty tytułu

PL

Powtarzalnosc parametrow biochemicznych i hematologicznych krwi oraz zrodla ich zmiennosci u bogatek

• Języki publikacji: EN

Abstrakty

EN

When interpreting responses to experimental manipulations or particular environmental cues, it is necessary to have previous knowledge about the natural variation of the response traits. We studied how nine blood parameters, including four enzymatic activities, varied with time in wild Great Tits by assessing their repeatabilities over periods of 45 days, in the same season (Spring or Autumn/Winter), in the same year and over a four years period. The accuracy of the measurements of these blood parameters was also assessed. Measurement error reflected essentially sample and time of storage rather than assay effects. Hematocrit and haemoglobin had low repeatabilities within Spring, ranging from 0.26 to 0.31; Heterophil/Lymphocyte ratio (H/L), white blood cell count (WBC), total plasma Cholinesterase and red blood cell glutathione peroxidase (GSH-Px) activities had moderate to high repeatabilities over periods of 45 days (repeatabilities ranged from 0.47 to 0.81 for GSH-Px and H/L, respectively), but also during longer periods such as during Spring (total plasma Cholinesterase activity) and Autumn/Winter (WBC, H/L and GSH-Px). Of the blood parameters measured, total plasma Cholinesterase, glutathione peroxidase and the haematological parameters WBC and H/L seem relatively constant and therefore reliable indicators of Great Tit's physiological condition within, at least, a 45 days time frame.

PL

Podczas badań prowadzonych na bogatkach badano w jaki sposób zmienia się w czasie 9 parametrów krwi — poziom cholinesterazy plazmatycznej, całkowity poziom cholinesterazy (ChE), butyrylocholinesterazy (BuChE), acetylocholinesterazy (AChE), peroksydazy czerwonych krwinek (GSH-Px) a także hematokryt (HCT), poziom hemoglobiny w czerwonych krwinkach (Hb), liczba białych krwinek (WBC) i stosunek heterofilów/leukocytów (H/L). Materiał pozyskiwano od bogatek podczas czterech lat badań (2003-2006) w Choupal, w środkowej Portugalii oraz kilku innych miejscach. W sumie zebrano od 213 do 246 próbek, przy czym 86- 95 dla tych samych osobników chwytanych ponownie. Ptaki były chwytane w sieci (jesienią i zimą) lub w skrzynkach podczas karmienia piskląt (w wieku 6-11 dni). Próbki krwi (100- 150 μl) pobierano z żyły skrzydłowej do kapilar zawierających heparynę. Przed rozpoczęciem badań sprawdzono także błąd pomiaru wynikający z zastosowanych procedur laboratoryjnych — używając krwi gołębia miejskiego (Tab. 1, 2). Okazało się, że u bogatek powtarzalność wyników u tych samych osobników chwytanych ponownie była znaczna, z wyjątkiem BuChE (zmienność 46%) oraz WBC (87%) i H/L (141%) (Tab. 3, Tab. 4). Wraz z wydłużeniem okresu między kolejnymi schwytaniami tego samego osobnika w danym sezonie powtarzalność wyników dla WBC, H/L oraz GSH-Px pozostawała średnia do wysokiej, jeśli powtórne odłowienie miało miejsce jesienią lub zimą. Wiosną powtarzalność dla WBC i H/L spadała. Gdy powtórne schwytania miały miejsce w różnych latach, powtarzalność pomiarów była jeszcze mniejsza. Dla HCT i Hb powtarzalność była niska na wiosnę, natomiast w przypadku białka utrzymywała się na średnim poziomie lecz spadała później w sezonie i w kolejnych latach. Podobne wyniki uzyskano też dla BuChE. Parametry związane z analizą leukocytów wykazywały znaczne podobieństwo, nawet w okresie jednego roku. Badania wskazują, że najbardziej wiarygodnymi wskaźnikami kondycji u bogatek są parametry hematologiczne (WBC oraz H/L) a także CHE i GSH-Px, które są relatywnie stabilne.

Słowa kluczowe

EN

condition; Parus major; ornithology; blood; Great Tit; glutathione peroxidase; plasma cholinesterase; health status; biochemical parameter; health profile; hematological parameter; bird

• Wydawca: -
• Czasopismo: Acta Ornithologica
• Rocznik: 2008
• Tom: 43
• Numer: 1
• Opis fizyczny: p.64-75,fig.,ref.

Twórcy

autor

Norte A C

• University of Coimbra, 3004-517 Coimbra, Portugal

autor

Sheldon B.

autor

Sousa J.P.

autor

Ramos J.A.

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