Wykorzystanie techniki PCR do typowania szczepow Haemophilus influenzae. I. Optymalizacja warunkow PCR w zastosowaniu do poszukiwania fragmentu genu bexA i fragmentu DNA swoistego dla H.Influenzae typu B

• Autorzy: Zasada A. A. , Paciorek J.
• Języki publikacji: PL

Abstrakty

PL

Wystandaryzowano warunki reakcji PCR do poszukiwania fragmentu genu bexA obecnego u wszystkich szczepów Haemophilus influenzae wytwarzających otoczki oraz dla fragmentu DNA swoistego dla szczepów H. influenzae typu b, uważanych za najbardziej zjadliwe. Wykrycie obecności ww fragmentów DNA w szczepach izolowanych z materiału klinicznego może być wysoce przydatne w typowaniu szczepów H. influenzae w przypadku uzyskania wątpliwych wyników metodami serologicznymi.

EN

PCR standardization was performed in order to detect a fragment of bexA gene, which is presented in all capsulate H. influenzae isolates, and a DNA fragment specific for H. influenzae type b. Implementing of such PCR into H. influenzae typing may be very useful in cases of isolates from clinical material for which serotyping alone gives unclear results. Standardization of PCR detecting DNA fragments specific for all capsular types will enable to perform complete typing of H. influenzae isolates.

Słowa kluczowe

PL

choroby bakteryjne; jama nosowo-gardlowa; Haemophilus influenzae; wirulencja; gen bexA; lancuchowa reakcja polimerazy; bakterie; DNA

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 4
• Opis fizyczny: s.357-363,tab.,fot.,bibliogr.

Twórcy

autor

Zasada A. A.

• Panstwowy Zaklad Higieny, Warszawa

autor

Paciorek J.

Bibliografia

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