Isolation, purification and identification of acetylcholine in Pharbitis nil seedlings

• Autorzy: Tretyn A , Lukasiewicz-Rutkowska H. , Kopcewicz J.
• Języki publikacji: EN

Abstrakty

EN

Acetylcholine (ACh), a well known animal neurotransmitter was isolated from tissues of Pharbitis nil using five different methods. Its presence in plant extract was confirmed by infrared spectroscopy and mass spectrometry. For quantitative estimation of ACh in P. nil seedlings pyrolysis-gas chromatography was applied. The presence of ACh was found in all organs of the examined plant: seeds, shoot apex, cotyledons, leaves, shoots and roots. However, the highest level of the investigated substance was noted in the youngest growing parts. In 5-day-old etiolated seedlings they were cotyledons, whereas in 14 day-old green plants - shoot apex and young leaves.

Słowa kluczowe

EN

acetylcholine; gas chromatography; isolation; pyrolysis; purification; Pharbitis nil; identification

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1997
• Tom: 19
• Numer: 3
• Opis fizyczny: p.303-309,fig.

Twórcy

autor

Tretyn A

• Nicholas Copernicus University, Gagarina 9, PL-87-100 Torun, Poland

autor

Lukasiewicz-Rutkowska H.

autor

Kopcewicz J.

Bibliografia

• Green J.P., Szilagyi I.A. 1974. Measurement of acetylcholine by pyrolysis gas chromatography. In: Hanin I. (Ed.), Choline and Acetylcholine: Handbook of Chemical Assay Methods, pp. 151–162. New York: Raven Press.
• Hanin I., Jenden D.J. 1969. Estimation of choline esters in brain by a new gas chromatographic procedure. Biochem. Pharmac., 18: 837–845.
• Hartmann E., Gupta R. 1989. Acetylcholine as a signaling system in plants. In: Boss W.E., Marre D.J. (Ed.), Second Messengers in Plant Growth and Development, pp. 257–288. New York: Alan R. Liss.
• Hartmann E., Kilbinger K. 1974a. Gas-liquid-chromatographic determination of light-dependent acetylcholine concentration in moss callus. Biochem. J., 137: 249–252.
• Hartmann E., Kilbinger K. 1974b. Occurence of light-dependent acetylcholine concentrations in higher plants. Experientia, 30: 1387–1388.
• Hasegawa Y., Kunihara M., Maruyama Y. 1982. Determination of picomole amounts of choline and acetylcholine in blood by gas chromatography-mass spectrometry equipped with a newly improved pyrolizer. J. Chrom., 239: 335–342.
• Hoshino T. 1983. Identification of acetylcholine as a natural constituent of Vigna seedlings. Plant Cell Physiol., 24: 829–834.
• Jaffe M.J. 1970. Ewidence for the regulation of phytochrome-mediated process in bean roots by the neurohumor, acetylcholine. Plant Physiol., 46: 768–777.
• Jenden D.J., Hanin I. 1974. Gas chromatographic microestimation of choline and acetylcholine after N-demethylation by sodium benzentiolate. In: Hanin I. (Ed.), Choline and Acetylcholine: Handbook of Chemical Assay Methods, pp. 135–150. New York: Raven Press.
• Kęsy J., Tretyn A., Łukasiewicz H., Kopcewicz J. 1991. Acetylcholinesterase from oat seedlings. I. Preliminary biochemical characterization of the enzyme. Biol. Plantarum, 33: 303–310.
• Lin R.C.I. 1957. Distribution of acetylcholine in the Malayan jack-fruit plant, Artrocarpus integra. Brit. J. Pharmacol., 12: 256–269.
• Łukasiewicz-Rutkowska H., Tretyn A., Cymerski M., Kopcewicz J. 1997. The effect of exogenous acetylcholine and other cholinergic agents on photoperiodic flower induction of Pharbitis nil. Acta Soc. Bot. Polon., 66: 47–54.
• Miura G.A., Shih T.-M. 1984. Cholinergic constituents in plants: characterization and distribution of acetylcholine and choline. Physiol. Plant., 61: 417–421.
• Maruyama Y., Kusaka M., Mori J., Horikawa A., Hasegawa Y. 1979. Simple method for the determination of choline and acetylcholine by pyrolisis gas chromatography. J. Chrom., 164: 121–127.
• Momonoki Y.S., Momonoki T. 1991. Changes in acetylcholine levels following leaf wiliting and recovery by heat stress in plant cultivars. Jap. Jour. Crop. Sci., 60: 283–290.
• Tretyn A., Kendrick R.E. 1991. Acetylcholine in plants: presence, metabolism and mechanism of action. Bot. Rev., 57: 33–73.
• Tretyn A., Tretyn M. 1990. Oscillations of acetylcholine in oat seedlings. Chronobiologia 17: 45–52.
• Tretyn A., Bobkiewicz W., Tretyn M., Michalski L. 1987. The identification of acetylcholine and choline in oat seedlings by gas chromatography and nuclear magnetic resonance (NMR). Acta Soc. Bot. Polon., 56: 499–511.