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2003 | 50 | 2 |

Tytuł artykułu

Ca2plus differently affects hydrophobic properties of guanylyl cyclase-activating proteins [GCAPs] and recoverin

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Guanylyl cyclase-activating proteins (GCAPs) and recoverin are retina-specific Ca2+ -binding proteins involved in phototransduction. We provide here evidence that in spite of structural similarities GCAPs and recoverin differently change their overall hydrophobic properties in response to Ca2+ . Using native bovine GCAP1, GCAP2 and recoverin we show that: i) the Ca2+ -dependent binding of recoverin to Phenyl-Se- pharose is distinct from such interactions of GCAPs; ii) fluorescence intensity of 1-anilinonaphthalene-8-sulfonate (ANS) is markedly higher at high [Ca2+ ]free (10 uM) than at low [Ca2+ ]freee(10 nM) in the presence of recoverin, while an opposing effect is observed in the presence of GCAPs; iii) fluorescence resonance energy transfer from tryptophane residues to ANS is more efficient at high [Ca2+ ]free in recoverin and at low [Ca2+ ]freee in GCAP2. Such different changes of hydrophobicity evoked by Ca2+ ap­pear to be the precondition for possible mechanisms by which GCAPs and recoverin control the activities of their target enzymes.

Wydawca

-

Rocznik

Tom

50

Numer

2

Opis fizyczny

p.367-376,fig.

Twórcy

  • Polish Academy of Sciences, R.Weigla 12, 53-114 Wroclaw, Poland
autor
autor
autor

Bibliografia

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Typ dokumentu

Bibliografia

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Identyfikator YADDA

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