EHDs are serine phosphoproteins: EHD1 phosphorylation is enhanced by serum stimulation

• Autorzy: Fichtman B , Ravid L. , Rapaport D. , Horowitz M.
• Języki publikacji: EN

Abstrakty

EN

Endocytic processes are mediated by multiple protein-protein interacting modules and regulated by phosphorylation and dephosphorylation. The Eps15 homology domain containing protein 1 (EHD1) has been implicated in regulating recycling of proteins, internalized both in clathrin-dependent and clathrin-independent endocytic pathways, from the recycling compartment to the plasma membrane. EHD1 was found in a complex with clathrin, adaptor protein complex-2 (AP-2) and insulin-like growth factor-1 receptor (IGF-1R), and was shown to interact with Rabenosyn-5, SNAP29, EHBP1 (EH domain binding protein 1) and syndapin I and II. In this study, we show that EHD1, like the other human EHDs, undergoes serine-phosphorylation. Our results also indicate that EHD1 is a serum-inducible serine-phosphoprotein and that PKC (protein kinase C) is one of its kinases. In addition, we show that inhibitors of clathrin-mediated endocytosis decrease EHD1 phosphorylation, while inhibitors of caveolinmediated endocytosis do not affect EHD1 phosphorylation. The results of experiments in which inhibitors of endocytosis were employed strongly suggest that EHD1 phosphorylation occurs between early endosomes and the endocytic recycling compartment.

Słowa kluczowe

EN

endocytosis; serine phosphoprotein; phosphorylation; serum stimulation; dephosphorylation; protein-protein interaction

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2008
• Tom: 13
• Numer: 4
• Opis fizyczny: p.632-648,fig.,ref.

Twórcy

autor

Fichtman B

• Tel-Aviv University, Ramat Aviv 69978, Israel

autor

Ravid L.

autor

Rapaport D.

autor

Horowitz M.

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