Differentiation of two dog hookworm species using PCR

• Autorzy: Mieszczanek J , Wedrychowicz H.
• Języki publikacji: EN

Abstrakty

EN

Reliable determination of hookworm nematode parasite species from dogs can be carried out post-mortem by microscopical examination of the buccal cavity of adult worms isolated from the host small intestine. In order to allow a proper diagnosis of the hookworm infection in a living host, we developed a polymerase chain reaction (PCR). Using DNA extracted from hookworm eggs and an oligonucleotide set designed on the basis of the DNA sequence of a cysteine proteinase AcCP1 gene, Ancylostoma caninum and Uncinaria stenocephala DNA can be discriminated from each other.

Słowa kluczowe

EN

DNA sequence; Ancylostoma caninum; infection; cat; hookworm; Uncinaria stenocephala; oligonucleotide; egg; parasite; semitropical region; dog; nematode; polymerase chain reaction; tropical region

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1999
• Tom: 44
• Numer: 1
• Opis fizyczny: p.81-83,fig.

Twórcy

autor

Mieszczanek J

• Warsaw Agricultural University, Grochowska 272, 03-849 Warsaw, Poland

autor

Wedrychowicz H.

Bibliografia

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