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2003 | 06 | 1 |

Tytuł artykułu

DSC, SDS-page and spectrophotometry for characterization of modified lysozyme

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was an attempt to apply selected analytical methods for the evaluation of preparations of modified lysozyme. Lysozyme isolated from hen egg white was modified using thermal, thermal-chemical, chemical and membrane methods and subsequently the obtained preparations were evaluated with the use of the spectrophotometric method, as well as electrophoresis and differential scanning calorimetry (DSC).

Wydawca

-

Rocznik

Tom

06

Numer

1

Opis fizyczny

http://www.ejpau.media.pl/series/volume6/issue1/food/art-11.html

Twórcy

  • August Cieszkowski Agricultural University of Poznan, Wojska Polskiego 31, 60-624 Poznan, Poland
autor

Bibliografia

  • Akashi A., 1972. The effect of egg white lysozyme on the growth of cheese starter organisms. J. Fac. Agric., 17, 67-69.
  • Cąkała S., 1994. Nowy preparat Lydium – KLP w leczeniu chorób zakaźnych zwierząt. [A new preparation Lydium-KLP in the treatment of contagious diseases of animals]. Życie Wet., 4A, 138-142 [in Polish].
  • Eisai Co. Ltd. 1972. Japanese patent, no. 5710/72.
  • Garbuliński T., 1994. Lydium – KLP w farmakoterapii weterynaryjnej. [Lydium-KLP in veterinary pharmacotherapy]. Życie Wet., 4A, 137 [in Polish].
  • Ibrahim H.R., Kato A., Kobayashi K., 1991. Antimicrobial effects of lysozyme against Gram-negative bacteria due to covalent binding of palmitic acid. J. Agric. Food Chem. 39, 2077-2082.
  • Ibrahim H.R., Hatta H., Fujiki M., Yamamoto T., 1994. Enhanced antimicrobial action of lysozyme against Gram-negative and Gram-positive bacteria due to modification with perillaldehyde. J. Agric. Food Chem., 42, 1813.
  • Ibrahim H.R., Higashiguchi S., Juneja L.R., Kim M., Yamamoto T., 1996. A structural phase of heat-denatured lysozyme with novel antimicrobial action, J. Agric. Food Chem., 44, 1416.
  • Jolles P., Jolles J., 1984. What is new in lysozyme research? Mol. Cell. Biochem., 63, 165.
  • Kiczka W., 1994. Od monomeru do dimeru lizozymu. [From lysozyme monomer to dimer]. Życie Wet., 4A, 131-136 [in Polish].
  • Kijowski J., Leśnierowski G., 1995. Wykorzystanie lizozymu do utrwalania żywności, w diagnostyce medyccnej I farmakologii. [The application of lysozyme to preserve food in medical diagnostic and pharmacology]. Biotechnologia, 2(29), 130 [in Polish].
  • Kijowski J., Leśnierowski G., Fabisz-Kijowska A., 1998. Methods of lysozyme separation, enzyme molecular form and functional quality of the residual egg white. Proc. 2nd Int. Symp. on Egg Nutrition and Newly Emerging Ovo-Technologies, Canadian Inovatech Inc., Banff, Alberta, Canada, 5-8 April 1998, p. 54.
  • Kijowski J., Leśnierowski G., 1999. Separation, polymer formation and antibacterial activity of lysozyme. Pol. J. Food Nut. Sci. 8/49(3), 3-16.
  • Kijowski J., Leśnierowski G., Fabisz-Kijowska A., 2000. Lysozyme polymer formation and functionality of residuals after lysozyme extraction. In: Egg Nutrition and Biotechnology. J. S. Sim, S. Nakai, W. Guenter (eds). International CAB, Wallingford, UK.
  • Laemmli U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680.
  • Leśnierowski G., 1997. Otrzymywanie lizozymu z białka jaja kurzego metodami krystalizacji, ultrafiltracji oraz z użyciem wymieniacza jonowego. [Isolation of lysozyme from hen egg white using the crystallization, ultrafiltration and ion exchange methods. Diss., Agr. Univ. Poznań. [in Polish].
  • Leśnierowski G., Cegielska-Radziejewska R., Kijowski J., 2001. Antibacterial activity of thermally modified lysozyme. EJPAU, 4(2), 1-9.
  • Leśnierowski G., Kijowski J., 1995. Metody badania aktywności enzymatycznej oraz oznaczanie ilościowe lizozymu z białka jaja kurzego. [Methods of investigating enzymatic activity and quantitative determination of lysozyme from hen egg white]. Przem. Spoż., 12, 476 [in Polish].
  • Nakamura S., Kato A., Kobayashi K., 1991. New antimicrobial characteristic of lysozyme-dextran conjugate. J. Agric. Food Chem., 39, 647.
  • Nakamura S., Kato A., Kobayashi K., 1992. Bifunctional lysozyme-galaktomannan conjugate having excellent emulsifying properties and bactericidal effect. J. Agric. Food Chem., 40, 735.
  • Proctor V.A., Cunningham F.E., 1988. The chemistry of lysozyme and its use as a food preservative and a pharmaceutical, CRC Crit. Rev. Food Sci. Nutr., 26(4), 359.
  • Sophianopoulos A.J., Van Holde K.E., 1964. Physical study of muramidase (lysozyme). J. Biol. Chem., 239, 2516.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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