Genomic DNA library of Veillonella parvula H2 in the rumen

• Autorzy: Liu G W , Zhang Z.G. , Yang W.Y. , Yang L.Y. , Wang Z.
• Języki publikacji: EN

Abstrakty

EN

To construct a genomic library of Veillonella parvula H2, total DNA was extracted, sheared by a Hydroshear machine, and the DNA fragments ligated a into Smal-digested vector pUC18 before being transformed into E. coli DH5a. Colonies were selected on Luria-Bertani (LB) plates containing ampicillin, 5-bromo-4-chloro-3-indoyl-3-galactose (X-gal), and isopropy-β-D-thiogalactoside (IPTG) and proliferated. Recombinant plasmids were analysed for the presence of inserted DNA fragments of 3-4 kb by restriction mapping. The titre of the library was determined to be 10⁵ pfu/mL according to the formula N=ln(l-p)/(l-f). The genomic library consisted of 99% of the genome of Veillonella parvula, demonstrating a successful library construction.

Słowa kluczowe

EN

rumen; Veillonella parvula; genomic library; plasmid; DNA fragment; dairy cow

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 1
• Opis fizyczny: p.15-17,fig.,ref.

Twórcy

autor

Liu G W

• College of Animal Husbandry and Veterinary Science, Jilin University, Changchun, 130062, China

autor

Zhang Z.G.

autor

Yang W.Y.

autor

Yang L.Y.

autor

Wang Z.

Bibliografia

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