Effect of chosen lactic acid bacteria strains on Staphylococcus aureus in vitro as well as in meat and raw sausages

• Autorzy: Gomolka-Pawlicka M. , Uradzinski J. , Wiszniewska A.
• Języki publikacji: EN

Abstrakty

EN

The present study was aimed at determining the influence of 15 strains of lactic acid bacteria on the growth of 2 Staphylococcus aureus strains in vitro as well as in meat and raw sausages. The investigations were performed within the framework of three alternate stages which differed in respect to the products studied, the number of Lactobacillus sp. strains and, partly, methodological approach. The study also considered water activity (aw) and pH of the products investigated. The results obtained are demonstrated in 5 diagrams. It was found that among 15 strains of Lactobacillus aureus investigated only one strain, Lactobacillus helveticus T 78, showed antagonistic effect on studied strains of Staphylococcus aureus both in vitro as well as in meat and raw sausages. Five other strains of Lactobacillus spp. displayed the antagonistic effect in vitro only. The temperature and incubation time of sausages, but also the type of sausage stuffing were found to have a distinct or slight influence, respectively, on the antagonistic interaction between the bacteria. However, this phenomenon was affected by neither aw nor pH.

Słowa kluczowe

EN

production; meat; antagonism; lactic acid bacteria; Staphylococcus aureus; raw sausage

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2004
• Tom: 07
• Numer: 4
• Opis fizyczny: p.251-259,fig.,ref.

Twórcy

autor

Gomolka-Pawlicka M.

• Warmia and Mazury University in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland

autor

Uradzinski J.

autor

Wiszniewska A.

Bibliografia

• Abrahim A, Papa A, Soultos N (1998) Antibiotic resistance of Salmonella spp. Isolates from traditionally made fresh sausage in Greece. J Food Prot 61: 1378-1380.
• Adesiyun AA (1995) Bacteriological quality of some Trynidadian ready-to-eat consume foods and drinks and possible health risk to consumers. J Food Prot 58: 651-655.
• Bover-Cid S, Izquierdo-Pulido M, Vidal-Carou MC (1999) Effect of proteolytic starter culture of Staphylococcus spp. On biogenic amine formation during the ripening of dry fermented sausages. Int J Food Microb 46: 95-104.
• Cantoni C, Butturini A, Magario G, Aloisi P (1994) Lactic acid bacteria in dry salami. Ind Aliment 33: 377-379.
• Caplice E, Fitzgerald GF (1999) Food Fermentations: role of microorganisms in food production and preservation. Int J Food Microb 50: 131-149.
• Carrasco MS, Garcia CC, Scarinci HE, Simonett AC (1999) Characterization of bacteriocin-like substances produced by Lactobacillus plantarum strains. Microb Alim Nutr 17: 49-57.
• Debevere J (1993) Use of starter cultures in the meat prod­ucts industry. Voedingsmiddelentechnologie 26: 13-17.
• El-Gohary AM (1994) Sausage and minced meat as a source of food poisining microorganisms to man. Assiut Vet Med J 30: 146-151.
• Encinas JP, Gonzalez CJ, Garcia-Lopez ML, Otero A (1999) Numbers and species of motile aeromonads during the manufacture of naturally contaminated Span­ish fermented sausages (longaniza and chorizo). J Food Prot 62: 1045-1049.
• Galuzzo L, Cliver DO (1996) Cutting boards and bacteria - oak vs. Salmonella. Dairy, Food and Environ Sanit 16: 290-293.
• Gimeno O, Ansorena D, Astiasaran I, Bello J (2000) Char­acterization of chorizo de Pamplona: instrumental measurements of colour and texture. Food-Chem 69: 195-200.
• Gomólka-Pawlicka M, Uradziñski J (2003a) Antagonistic effect of chosen lactic acid bacteria strains on Salmonella species in meat and fermented sausage. Pol J Vet Sci 6: 29-39.
• Gomólka-Pawlicka M, Uradziñski J (2003b) Antagonistic effect of chosen lactic acid bacteria strains on Yersinia enterocolitica species in model set-ups, meat and fer­mented sausage. Pol J Vet Sci 6: 99-108.
• Hastings JW, Stiles ME, Holy A (1994) Bacteriocins of leu- conostocs from meat. Int J Food Microb 24: 75-81.
• Hechelmann H, Kasprowiak R (1991) Mikrobio- logische Kriterien ftir stabile Produkte. Fleischwirtschaft 71: 374-389.
• Hugenholtz J (1998) Bacteriocins as preservatives in food. Bulletin of the International Dairy Federation 329: 6-8.
• Jakubczak A, Bukowski K (2000) Antagonistyczne działanie bakterii fermentacji mlekowej w stosunku do wybranych drobnoustrojów. Medycyna Wet 56: 53-57.
• Kornacki K (1996) Zastosowanie kultur starterowych to be­zpieczeństwo spożycia kiełbas fermentowanych. Przemyśl Spożywczy 3: 35-36.
• Kuri V, Madden RH, Collins MA (1996) Hygienic quality of raw pork and chorizo (raw pork sausage) on retail sale in Mexico City. J Food Prot 59: 141-145.
• Leroy F, Vuyst L (1999a) Temperature and pH conditions that prevail during fermentation of sausages are optimal for production of the antilisterial bacteriocin sakacin K. Appl and Environm Microb 65: 974-981.
• Leroy F, Vuyst L (1999b) The presence of salt and a curing agent reduces bacteriocin production by Lactobacillus sakei CTC 494, a potential starter culture for sausage fermentation. Appl Evironment Microb 65: 5350-5356.
• Liepe HU (1976) Die praktische Anwendung von Starten- kulturen bei Rohwurst. Fleischwirtschaft 56: 180-183.
• Mikami M, Kawasima H, Sekikawa M (1998) Studies on some properties of dry fermented sausages with bacterial starter cultures. Anim Sci and Technol 69: 53-61.
• Nichols GLS, de Louvois J (1987) The microbiological qual­ity of raw sausage sold in U.K. PHLS Microbiol Digest 12: 236-242.
• Pacini R, Mallogi L, Tozzi E, Gallasi R (1994) Pathogen bacteria isolated from meat and meat products. Ind Ali­ment 33: 945-950.
• Park WM, Choi WH, Yoo IJ, Kim WJ, Jeon KH, Chung DH (1997) Effects of lactic acid bacteria isolated from fer­mented foods on the microbiological properties of fer­mented sausages during storage. Korean J Anim Sci 39: 59-64.
• Polska Norma PN-A-82055-3. Mięso i przetwory mięsne. Badania mikrobiologiczne. Przygotowanie próbek i roz- cieńczeń.
• Polska Norma PN-A-82055-9. Mięso i przetwory mięsne. Badania mikrobiologiczne. Wykrywanie obecności i oz­naczanie liczby Staphylococcus aureus.
• Schilinger U, Geisen R, Holzapfel WH (1996a) Potential of antagonistic microorganisms and bacteriocins for the bio­logical preservation of foods. Trends in Food Sci Technol 7: 158-164.
• Schillinger U, Holzapfel WH (1996b) Guidelinaes for manuscripts on lactic acid bacteria. Int J Food Microbiol 33, iii-v.
• Schillinger U, Chung HS, Keppler K, Holzapfel WH (1998) Use of bacteriocinogenic lactic acid bacteria to inhibit spontaneous nisin-resistant mutants of Listeria mono­cytogenes Scott A. Journal of Applied Microbiology 85: 657-663.
• Schillinger U, Lücke FK (1989) Lactic acid as protective cultures for use meat products. Fleischwirtschaft 69: 1581-1585.
• Schillinger U, Lücke FK (1990) Lactic acid bacteria as pro­tective cultures in meat products. Fleischwirtschaft 70: 1296-1299.
• Spelhaug SR, Harlander S (1989) Inhibition of foodborne bacterial pathogens by bacteriocins from Lactococcus lac- tis and Pediococcus pentosaceus. J Food Prot 52: 856-862.
• Stolle A, Greil B, Stock K (1999) Mikrobiologiczna kontrola stanu mielonego mięsa - możliwość redukcji poziomu salmonelli w rzeźniach. Materiały sympozjum naukowego „Zdrowa Żywność” - Pro Animali - Wroclaw.
• Torner MJ, Castillo M, Pla S (1995) A study of the sanitary quality of fresh meat products manufactured in meat pro­cessing plant in the health area of Xativa. Alimentaria 262: 27-31.
• Usajewicz I (1998) Bakterie fermentacji mlekowej. Klasyfikacja, Metabolizm, Genetyka, Wykorzystanie. Monografia Wyd Polit Łódzkiej: 123-137.
• Vorster SM, Greebe RP, Nortje GL (1994) Incidence of Staphylococcus aureus ndd E. coli in grind beef, broilers and processed meat in Pretoria, South Africa. J Food Prot 57: 305-310.
• Wojtoń B, Różańska H, Różycki M (1997) Mik­robiologiczne zanieczyszczenia żywności pochodzenia zwierzęcego w Polsce. Medycyna Wet 53: 332-336.