Effects of pineal gland and melatonin on maturation gonadotropin [GtH2] secretion from perifused pituitary glands of mature carp during spawning

• Autorzy: Popek W. , Luszczek-Trojnar E. , Epler P.
• Języki publikacji: EN

Abstrakty

EN

The study, carried out during the spawning season, involved 30 mature carp females aged 5 years. The fish were divided into two experimental groups, those belonging to one of them being injected pimozide, a dopamine antagonist. The pituitaries and pineals were removed from all the females and perifused. Perifusion was carried out in pure medium alone, in the medium in the presence of the pineal gland, and in the medium enriched with dissolved melatonin. The perifusion proceeded in 2000 lux intensity light and in total darkness. Maturation gonadotropin (GtH2) concentration in the perifusion medium pumped through columns was determined with ELISA, an immunoenzymatic technique. The experimental treatments within each group showed no significant differences (p>0.01) with respect to the GtH2 concentrations in the perifusion medium. This result can be regarded as an evidence of a lack of any direct effect of the pineal gland and/or melatonin on GtH2 secretion from the carp pituitary during spawning.

Słowa kluczowe

EN

melatonin; gonadotrophin secretion; endocrine maturation control; carp; fish; pituitary gland; maturation; spawning season; spawning; pineal gland; female

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Fisheries
• Rocznik: 2000
• Tom: 03
• Numer: 1
• Opis fizyczny: http://www.ejpau.media.pl/series/volume3/issue2/fisheries/art-01.html,fig.

Twórcy

autor

Popek W.

• Agricultural University in Krakow, Krakow, Poland

autor

Luszczek-Trojnar E.

autor

Epler P.

Bibliografia

• Breton B., Mikolajczyk T., Popek W., 1993, The neuroendocrine control of the gonadotropin (GtH2) secretion in teleost fish. In: Aquaculture: Fundamental and Applied Research (B. Lahlou and P. Vitiello Eds.) pp. 199-215. American Geophysical Union. U.S.A.
• Breton B., Mikolajczyk T., Popek W., Bieniarz K. Epler P., 1991, Neuropeptide Y stimulates in vivo gonadotropin secretion in teleost fish. Gen.Comp.Endocrinol. 84, 277-273.
• Chang J.P., Peter R.E., 1983, Effects of dopamine on gonadotropin release in goldfish, Carassius auratus. Gen. Comp. Endocrinol. 49, 22-31.
• Chang J.P., Van Goof F., Acharya S., 1991, Influences of norepinephrine, and adrenergic agonist and antagonist on gonadotropin secretion from dispersed pituitary cells of goldfish, Carassius auratus. Neuroendocrinol. 54, 202-210.
• Costa E.J.X., Lopes R.H., Lamy-Freund M.T., 1995, Permeability of pure lipid bilayers to melatonin. Pineal Res. 19, 123-126.
• Cwioro E., Popek W., Luszczek-Trojnar E., 1998, The effects of temperature on the melatonin secretion from superfused carp pineals in spawning time. 7th International Symposium of Polish Network of Molecular and Cellular Biology UNESCO/PAS “Molecular and Physiological Aspects of Regulatory Processes of the Organism”. Krakow, 22 - 23 June 1998. Materials, 63 - 64.
• Hontela A., Peter R.E., 1980, Effects of pinealectomy, blinding and sexual condition on serum gonadotropin levels in the goldfish. Gen. Comp. Endocrinol. 40, 168-179.
• Jalabert B., Szollosi D., Fostier A., 1973, Étude comparé de l’ action des hormones hypophysaires et steroides sur la maturation in vitro de ovocytes de la Truite et du Carassin (Poissons téléosténs). Ann. Biol. Anim. Biochim. Biophys. 13, 59-72.
• Kah O., Pontet A., Rodriguez J., Calas A., Breton B., 1989, Development of an enzyme-linked immunosorbent assay for goldfish gonadotropin. Biol. Reprod. 40, 68-73.
• Kezuka, H., Furukawa, K., Aida, K., Hanyu, I., 1988, Daily cycles in plasma melatonin under long or short photoperiod in the common carp, Cyprinus carpio. Gen. Comp. Endocrinol. 72, 296-302.
• Lincoln G.A., 1992, Administration of melatonin into the mediobasal hypothalamus as a continuous or intermittent signal effects the secretion of follicle stimulating hormone and prolactin in the ram. J. Pineal Res. 12, 135-144.
• Malpaux B., Daveau A., Maurice F., Locatelli A., Thierry J.C., 1994, Evidence that melatonin binding sites in the pars tuberalis do not mediate the photoperiodic actions of melatonin on LH and prolactin secretion in ewes. J. Reprod. Fertil. 101, 625-632.
• Maywood E.S., Hastings M.H., 1995, Lesions of the iodomelatonin-binding sites of the mediobasal hypothalamus spare the lactotropic, but block the gonadotropic response of male Syrian hamsters to short photoperiod and to melatonin. Endocrinology 136, 144-153.
• Maywood E.S., Bittman E.L., Hastings M.H., 1996, Lesions of the melatonin - and androgen-responsive tissue of the dorsomedial nucleus of the hypothalamus block the gonadal response of male Syrian hamsters to programmed infusions of melatonin. Biol. Reprod. 54, 470-477.
• Mazurais D., Brierley I., Anglade I., Drew J., Randall C., Bromage N., Michel D., Kah O., Williams L.M., 1999, Central melatonin receptors in the rainbow trout: Comperative distribution of ligand binding and gene expression. J. Comp. Neurol. 409, 313-324.
• Peter R.E., Trudeau V.L., Sloley B.D., 1991, Brain regulation of reproduction in teleost. Bull. Inst. Zool., Academia Sinica, Monograph 16, 89-118.
• Peter R.E., Trudeau V.L., Sloley B.D., Peng C., Nahorniak C.S., 1991: Actions of catecholamines, peptides and sex steroids in regulation of gonadotropin-II in the goldfish. In: “Reproductive Physiology of Fish”. (A.P. Scott, J.P. Sumpter, D.E. Kime and M.S. Rolfe Eds.), 30-34, Sheffield.
• Popek W., Epler P., 1999, Effects of intraventricular melatonin microinjections on hypothalamic catecholamine activity in carp females during a year. Electronic Journal of Polish Agricultural Universities (www.ejpau.media.pl), 2 (1) Fisheries.
• Popek W., Bieniarz K., Epler P., 1991, Role of pineal gland in sexual cycle in common carp. In: "Chronobiology and Chronomedicine" (J. Surowiak and M.H. Lewandowski Eds.), 99-102. Verlag Peter Lang, Frankfurt.
• Popek W., Epler P., Sokolowska-Mikolajczyk M., 1996, Melatonin does not affect steroidogenesis or maturation of carp oocytes in vitro during the prespawning period. Pol. Arch. Hydrobiol. 43(3), 379-385.
• Popek W., Galas J., Epler P., 1997, The role of pineal gland in seasonal changes of blood estradiol level in immature and mature carp females. Arch. Pol. Fish. 5(2), 259-265.
• Popek W., Breton B., Sokolowska-Mikolajczyk M., Epler P., 1994, The effects of bicuculline (a GABA receptor antagonist) on LHRH-A and pimozide stimulated gonadotropin (GtH2) release in female carp (Cyprinus carpio L.). Comp. Biochem. Physiol. 108/1, 129-135.
• Popek W., Epler P., Bieniarz K., Sokolowska-Mikolajczyk M., 1997, Contribution of factors regulating melatonin release from pineal gland of carp (Cyprinus carpio L.) in normal and in polluted environments. Arch. Pol. Fish. 5(1), 59-75.
• Popek W., Breton B., Piotrowski W., Bieniarz K., Epler P., 1994, The role of the pineal gland in the control of a circadian pituitary gonadotropin release rhythmicity in mature female carp. Neuroendocrinol. Lett. 16(3), 183-193.
• Reiter R.J., 1995, Functional pleiotropy of the neurohormone melatonin: antioxidant protection and neuroendocrine regulation. Frontiers in Neuroendocrinology 16, 383-415.
• Reppert S.M., Weaver D.R., Ebisawa T., 1994, Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. Neuron 13, 1177-1185.
• Rosenblum P.M., Peter R.E., 1989, Evidence for the involvement of endogenous opioids in the regulation on gonadotropin secretion in male goldfish Carassius auratus. Gen. Comp. Endocrinol. 73, 21-27.
• Shida, C.S., Castrucii, A.M.L., Lamy-Freund, M.T., 1994, High melatonin solubility in aquas medium. J. Pineal Res. 16, 198-201.
• Somoza G.M., Peter R.E., 1991, Effects of serotonin on gonadotropin and growth hormone release from in vitro perifused goldfish pituitary fragments. Gen. Comp. Endocrinol. 82, 103-110.
• Somoza G.M., Yu K.L., Peter R.E., 1988, Serotonin stimulates gonadotropin release in female and male goldfish, Carassius auratus L. Gen. Comp. Endocrinol. 72, 374-382.
• Stankov B., Fraschini F., Reiter R.J., 1993, Melatonin receptors: distribution, biochemistry and pharmacology. In: “Melatonin: Biosynthesis, Physiological Effects and Clinical Application”. (H.-S. Yu, R.J. Reiter, Eds), 155-186. CRC Press, Boca Raton, London.