Enzymy hydrolityczne entomopatogennych grzybow i ich rola w patogenezie

• Autorzy: Kolaczkowska M , Chrzanowska J. , Bania J.
• Języki publikacji: PL

Abstrakty

EN

Entomopathogenic fungi attack the host insect and therefore may be potentially used for insect control. Penetration of cuticle is achieved by a combination of mechanical pressure and enzymatic degradation. The entomopathogenic fungi produce a variety of exocellular hydrolytic enzymes: proteases, chitinases and lipases, which are capable to digest the major components of insect cuticle. This review describes the characteristics of these enzymes and their role in pathogenesis.

Słowa kluczowe

PL

grzyby entomopatogenne; Metarhizium anisopliae; enzymy proteolityczne; enzymy hydrolityczne; Beauveria bassiana; wirulencja

• Wydawca: -
• Czasopismo: Postępy Nauk Rolniczych
• Rocznik: 1997
• Tom: 44
• Numer: 6
• Opis fizyczny: s.31-44,tab.,bibliogr.

Twórcy

autor

Kolaczkowska M

• Uniwersytet Wroclawski, Wroclaw

autor

Chrzanowska J.

autor

Bania J.

Bibliografia

• [1] Bidochka M.J., Khachatourians G.G. 1987. Purification and properties of an extracellular protease produced by the entomopathogenic fungus Beauveria bassiana. Appl. Environ. Microbiol. 53: 1679-1684.
• [2] Bidochka M.J., Khachatourians G.G. 1988. Regulation of extracellular protease in the entomopathogenic Fungus Beauveria bassiana. Exp. Mycol. 12: 161-168.
• [3] Bidochka M.J., Khachatourians G.G. 1990. Identification of Beauveria bassiana extracellular protease as a virulence factor in pathogenicity toward the migratory grasshopper, Melanoplus sanguinipes. J. Invert. Pathol. 56: 362-370.
• [4] Bidochka M.J., Khachatourians G.G. 1992. Growth of the entomopathogenic fungus Beauveria bassiana on cuticular components from the migratory grasshopper, Melanoplus sanguinipes. J. Invert. Pathol. 59: 165-173.
• [5] Bidochka M.J., Khachatourians G.G. 1994. Protein hydrolysis in grasshopper cuticles by entomopathogenic fungal extracellular proteases. J. Invert. Pathol. 63: 7-13.
• [6] Bidochka M.J., Khachatourians G.G. 1994. Basic proteases of entomopathogenic fungi differ in their adsorption properties to insect cuticle. J. Invert. Pathol. 64: 26-32.
• [7] Chrzanowska J., Banaś J., Bania J., Kołaczkowska M. 1996. Pozakomórkowe enzymy proteolityczne entomopatogennego grzyba Beauveria bassiana. Materiały XXXII Zjazdu PTBioch. Kraków: 199.
• [8] Chrzanowska J., Blom H. 1993. Aminopeptidase activity of Beauveria bassiana. Materiały XXIX Zjazdu PTBioch. Wrocław: 503.
• [9] Cole S.C., Charnley A.K., Cooper R.M. 1993. Purification and partial characterization of a novel trypsin-like cysteine protease from Metarhizium anisopliae. FEMS Microbiol. Letters 113: 189-196.
• [10] Dean D.D., Domnas A.J. 1983. The extracellular proteolytic enzymes of the mosquito-parasitizing fungus Lagenidium giganteum. Exp. Mycol. 7: 31-39.
• [11] Goettel M. SW., St Leger R.J., Rizzo N.W., Staples R.C., Roberts D.W. 1989. Ultrastructural localization of a cuticle-degrading protease produced by the entomopathogenic fungus Metarhizium anisopliae during penetration of host (Manduca sexta) cuticle. J. Gen. Microbiol. 135: 2233-2239.
• [12] Gunkel F.A., Gassen H.G. 1989. Proteinase K from Tritirachium album Limber. Characterization of the chromosomal gene and expression of the cDNA in Escherichia coli. Eur. J. Biochem. 179: 185-194.
• [13] Gupta S.C., Leathers T.D. El-Sayed G.N., Ignoffo C.M. 1992. Insect cuticle-degrading enzymes from the entomogenous fungus Beauveria bassiana. Exp. Mycol. 16: 132-137.
• [14] Havukkala I., Mitamura C., Hara S., Hiragae K., Nishizawa Y., Hibi T. 1993. Induction and purification of Beauveria bassiana chitinolytic enzymes. J. Invert. Pathol. 61: 97-102.
• [15] Hegedus D.D., Khachatourians G.G. 1988. Production of an extracellular lipase by Beauveria bassiana. Biotech. Letters 10: 637-642.
• [16] Hurion R., Fromentin H., Keil B. 1979. Specificity of the collagenolytic enzyme from the fungus Entomophtora coronata: Comparison with the bacterial collagenase from Achromobacter iophagus. Arch. Biochem. Biophys. 192: 438-445.
• [17] Joshi K., St Leger R.J., Bidochka M.J. 1995. Cloning of the cuticle -degrading protease from the entomopathogenic fungus, Beauveria bassiana. FEMS Microbiol. Letters 125: 211-218.
• [18] Mollier P., Lagnel J., Fournet B., Aioun A., Riba G. 1994. A glycoprotein highly toxic for Galleria mellonella Larvae secreted by the entomopathogenic fungus, Beauveria sulfurescens. J. Invert. Pathol. 64: 200-207.
• [19] Paterson I.C., Charnley A.K., Cooper R.M., Clarkson J.M. 1993. Regulation of production of a trypsin-like protease by the entomopathogenic fungus Metarhizium anisopliae. FEMS Microbiol. Letters 109: 323-328.
• [20] Paterson I.C., Cooper R.M., Charnley A.K., Clarkson J.M. 1994. Partial characterization of specific inducers of a cuticle degrading protease from the insect pathogenic fungus Metarhizium anisopliae. Microbiology 140: 3153-3159.
• [21] Robert A., Messing-Al-Aidroos K. 1985. Acid production by Metarhizium anisopliae: effects on virulence againsts mosquitoes and on detection of in vitro amylase, protease and lipase activity. J. Invert. Pathol. 45: 9-15.
• [22] Samuels R.I., Paterson I.C. 1995. Cuticle degrading proteases from insect moulting fluid and culture filtrates of entomopathogenic fungi. Comp. Biochem. Physiol. 110: 661-669.
• [23] St Leger R.J., Bidochka M.J., Roberts D.W. 1994. Isoforms of the cuticle degrading Pr1 proteinase and production of a metalloproteinase by Metarhizium anisopliae. Arch. Biochem. Biophys. 313: 1-7.
• [24] St Leger R.J., Bidochka M.J., Roberts D.W. 1994. Characterization of a novel carboxypeptidase produced by the entomopathogenic fungus Metarhizium anisopliae. Arch. Biochem. Biophys. 314: 392-398.
• [25] St Leger R.J., Butt T.M., Goettel M.S., Roberts D.W., Staples R.C. 1989. Synthesis of proteins including a cuticle-degrading protease during differentiation of the entomopathogenic fungus Metarhizium anisopliae. Exp. Mycol. 13: 253-262.
• [26] St Leger R.J., Charnley A.K., Cooper R.M. 1986. Cuticle-degrading enzymes of entomopathogenic fungi: synthesis in culture on cuticle. J. Invert. Pathol. 48: 85-95.
• [27] St Leger R.J., Charnley A.K., Cooper R.M. 1986. Cuticle-degrading enzymes of entomopathogenic fungi: Mechanisms of interaction between pathogen enzymes and insect cuticle. J. Invert. Pathol. 47: 295-302.
• [28] St Leger R.J., Charnley A.K., Cooper R.M. 1987. Characterization of cuticle-degrading proteases produced by the entomopathogen Metarhizium anisopliae. Arch. Biochem. Biophys. 253: 221-232.
• [29] St Leger R.J., Cooper R.M., Charnley A.K. 1986. Cuticle-degrading enzymes of entomopathogenic fungi: Cuticle degradation in vitro by enzymes from entomopathogens. J. Invert. Pathol. 47: 167-177.
• [30] St Leger R.J., Cooper R.M., Charnley A.K. 1986. Cuticle-degrading enzymes of entomopathogenic fungi: regul ation of production of chitinolytic enzymes. J. Gen. Microbiol. 132: 1509-1517.
• [31] St Leger R.J., Cooper R.M., Charnley A.K. 1987 Distribution of chymoelastases and trypsin-like enzymes in five species of entomopathogenic Deuteromycetes. Arch. Biochem. Biophys. 258:123-131.
• [32] St Leger R.J., Cooper R.M., Charnley A.K. 1987. Production of a cuticle degrading enzymes by the entomopathogen Metarhizium anisopliae during infection of cuticles from Calliphora vomitoria and Manduca sexta. J. Gen. Microbiol. 133: 1371-1382.
• [33] St Leger R.J., Cooper R.M., Charnley A.K. 1993. Analysis of aminopeptidase and dipeptidylpeptidase IV from the entomopathogenic fungus Metarhizium anisopliae. J. Gen. Microbiol. 139: 237-243.
• [34] St Leger R.J., Durrands P.K., Charnley A.K., Cooper R.M. 1988. Role of extracellular chymoelastase in the virulence of Metarhizium anisopliae for Manduca sexta. J. Invert. Pathol. 52: 285-294.
• [35] St Leger R.J., Durrands P.K., Charnley A.K., Cooper R.M. 1988. Regulation of production of proteolyticenzymes by the entomopathogen Metarhizium anisopliae. Arch. Microbiol. 150: 413-416.
• [36] St Leger R.J., Frank R.J., Roberts D.C.,Staples R.C. 1992. Molecular cloning and regulatory analysis of the cuticle degrading protease structural gene from the entomopathogenic fungus Metarhizium anisopliae. Eur. J. Biochem. 204: 991-1001.
• [37] St Leger R.J., Joshi L., Bidochka M.J., Rizzo N.W., Roberts D.W. 1996. Biochemical characterization and ultrastructural localization of two extracellular trypsins produced by Metarhizium anisopliae in infected insect cuticles. Appl. Environ. Microbiol. 62: 1257-1264.
• [38] St Leger R.J., Joshi L., Bidochka M.J., Rizzo N.W., Roberts D.W. 1996. Characterization and ultrastructural localization of chitinases from Metarhizium anisopliae, M. flavoviride and Beauveria bassiana during fungal invasion of host (Manduca sexta) cuticle. Appl. Environ. Microbiol. 62: 907-912.
• [39] St Leger R.J., Joshi L., Bidochka M.J., Roberts D.W. 1995. Multiple aminopeptidases produced by Metarhizium anisopliae. J. Invert. Pathol. 65: 313-314.
• [40] Thomas K.C., Khachatourians G.G., Ingledew W.M. 1987. Production and properties of Beauveria bassiana conidia cultivated in submerged culture. Can. J. Microbiol. 33: 12-20.
• [41] Willis J.H. 1987. Cuticular proteins. The neglected components. Arch. Insect. Biochem. Physiol. 6: 203-215.
• [42] Zacharuk R.Y. 1970. Fine structure of the fungus Metarhizium anisopliae infecting three species of larval Elateridae (Coleoptera). III. Penetration of the host integument. J. Invert. Pathol. 15: 372-396.