Polimorfizm genu PRNP w kodonach 136, 154 i 171 u polskich ras owiec

• Autorzy: Niznikowski R , Luhken G. , Strzelec E. , Lipsky S. , Popielarczyk D. , Erhardt G. , Ajmone-Marsan P. , Bruford M. , Dunner S. , Obexer-Ruff G. , Taberlet P. , Valentini A. , Caloz R. , Georgudis A. , Canali G. , Roosen J. , Crepaldi P. , Togan I. , Vlaic A. , Fesus L. , Ertugrul O. , Abo-Shekada M. , El Barody M. , Hoda A. , Trommetter M.

Warianty tytułu

EN

PRNP gene polymorphism in 136, 154 and 171 codons in Polish sheep breeds

• Języki publikacji: PL

Abstrakty

EN

The study was conducted on six Polish sheep breeds (Polish merino, Zelaznenska sheep, Pomeranian, Kamieniecka sheep, Polish mountain sheep and Polish heath sheep). Individual blood samples were collected and DNA was extracted. The frequencies of alleles (ARR, ARH, ARQ, AHQ, VRQ) and its genotypes of PRNP gene were determined according to the RFLP-PCR method. Depending on the breed and sex, a high diversity of polymorphous forms of the PRNP gene was observed. Higher PRNP gene polymorphism was observed in ewes than in rams. The lowest frequency of alleles was determined in three breeds: Polish merino (ARR, ARQ and VRQ), Polish mountain sheep and Polish heath sheep (ARR, ARQ and AHQ). All five of the determined alleles were observed in Pomeranian and Kamieniecka sheep. The most advantageous frequencies of ARR allele occurred in Polish merino, Kamieniecka and Zelaznenska sheep (particularly in ewes). In Polish mountain sheep and Polish heath sheep the VRQ allele was not observed. In the final results, 11 combinations of genotypes occurred in six Polish sheep breeds. The obtained results indicate a need for the construction of an appropriate breeding program for each breed separately. These breeding programs should enable obtaining higher frequencies of ARR allele occurrence and restricting the incidence of other alleles of the PRNP gene, particularly VRQ allele.

Słowa kluczowe

PL

polimorfizm; rasy zwierzat; choroby zwierzat; owce; gen PRNP; scrapie

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 08
• Opis fizyczny: s.938-941,rys.,tab.,bibliogr.

Twórcy

autor

Niznikowski R

• Szkola Glowna Gospodarstwa Wiejskiego, ul.Ciszewskiego 8, 02-786 Warszawa

autor

Luhken G.

autor

Strzelec E.

autor

Lipsky S.

autor

Popielarczyk D.

autor

Erhardt G.

autor

Ajmone-Marsan P.

autor

Bruford M.

autor

Dunner S.

autor

Obexer-Ruff G.

autor

Taberlet P.

autor

Valentini A.

autor

Caloz R.

autor

Georgudis A.

autor

Canali G.

autor

Roosen J.

autor

Crepaldi P.

autor

Togan I.

autor

Vlaic A.

autor

Fesus L.

autor

Ertugrul O.

autor

Abo-Shekada M.

autor

El Barody M.

autor

Hoda A.

autor

Trommetter M.

Bibliografia

• 1.O'Doherty E., Aherne M., Ennis S., Weavers E., Roche J. F., Sweeney T.: Prion protein gene polymorphisms in pedigree sheep in Ireland. Res. Vet. Sci. 2001, 70, 51-56.
• 2.Gombojav A., Ishiguro N., Horiuchi M., Serjmyadag D., Byambaa B., Shinagawa M.: Amino acid polymorphisms of PrP gene in Mongolian Sheep. J. Vet. Med. Sci. 2003, 65, 75-81.
• 3.Kaal L., Windig J.: Rare sheep breeds and breeding for scrapie resistance in the Netherlands. 56th Annual Meeting of European Association of Animal Production (EAAP), 5-8.06.2005, Uppsala, Sweden 2005, 11, 375.
• 4.Kaam J. B. C. H. M. van, Finocciaro R., Vitale M., Portolano B., Vitale F., Caracappa S.: PrP allele frequencies in non-infected Valle del Belice and infected cross-bred flocks. 56th Annual Meeting of European Association of Animal Production (EAAP), 5-8.06.2005, Uppsala, Sweden 2005, 11, 376.
• 5.Lühken G., Buschmann S., Groschup M. H., Erhardt G.: Prion protein allele A136H154Q171 is associated with high susceptibility to scrapie in purebred and crossbred German Merinoland sheep. Arch. Virol. 2004, 149, 1571-1580.
• 6.Matthews D.: Scrapie an overview; polisy issues and potential eradication measures. 56th Annual Meeting nof European Association of Animal Production (EAAP), 5-8.06.2005, Uppsala, Sweden 2005, 11, 374.
• 7.Regulacja nr 999/2001/EC: Reguły prawne z zakresu zapobiegania, kontroli i zwalczania pasa¿owalnych encefalopatii gąbczastych. Unia Europejska, Bruksela 2001.
• 8.Regulacja nr 2003/100/EC: Decyzja Komisji Europejskiej w sprawie ustanowienia obowiązku tworzenia schematów hodowlanych prowadzących do zwiększenie genetycznej oporności na trzęsawką u każdej z ras owiec w Europie. Unia Europejska, Bruksela 2003.
• 9.Regulacja nr 260/2003/EC.: Ustanowiono obowiązek zwalczania TSE u owiec i kóz oraz uregulowano handel żywymi owcami i kozami bydlęcymi. Unia Europejska, Bruksela 2003.