Changes in the cellular behaviour of human colonic cell line Caco-2 in response to butyrate treatment

• Autorzy: Dzierzewicz Z. , Orchel A. , Weglarz L. , Latocha M. , Wilczok T.
• Języki publikacji: EN

Abstrakty

EN

Gut-derived adenocarcinoma Caco-2 cells were treated with sodium butyrate (NaB) at physiologically relevant concentrations. We characterized its effects on prolifera­tion, differentiation, apoptosis, adhesion to the solid support and interleukin-8 secre­tion. Differentiation was determined by brush border alkaline phosphatase activity. Apoptosis was assessed by acridine orange and Hoechst stains. Differentiation and apoptosis were analyzed in both adherent and floating cell populations. The trans­formed Caco-2 cells did not retain their malignant phenotype in the presence of NaB. They appeared to undergo a change in the phenotype induced by NaB, as indicated by reduced proliferation, enhanced differentiation, stimulation of apoptosis leading to decreased viability of cells, and stimulation of interleukin-8 secretion. Considering all the above facts and data, we postulate that Caco-2 cells cultured in NaB supplemented medium could regain the phenotypic characteristics of the phenotype of the parent cell from which originated the Caco-2 line.

Słowa kluczowe

EN

Caco-2 cell; butyrate; colonic cell line; adenocarcinoma; cell behaviour; sodium butyrate; interleukin 8; cell line; butyric acid; man; treatment; apoptosis; differentiation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 1
• Opis fizyczny: p.211-220,fig.

Twórcy

autor

Dzierzewicz Z.

• Silesian Medical University of Silesia, Sosnowiec, Poland

autor

Orchel A.

autor

Weglarz L.

autor

Latocha M.

autor

Wilczok T.

Bibliografia

• 1.Wolin, M.J. (1994) Control of short chain volatile acid production in the colon; in Short-chain Fatty Acids (Cummings, J., Binder, H.J. & Soergel, K., eds.) pp. 3-10, Kluwer Academic Publishers, Boston.
• 2.Dzierzewicz, Z., Kwapisz, I., Cwalina, B. & Wilczok, T. (1999) The role of butyric acid in growth, proliferation and differentiation of colonocytes. Gastroenterol. Pol. 6, 153-159.
• 3.Gibson, P.R., Moeller, J., Kagelari, O., Folino, M. & Young, G.P. (1991) Contrasting effects of butyrate on expression of phenotypic markers of differentiation in neoplastic and non-neoplastic colonic epithelial cells in vitro. J. Gastrol. Hepatol. 7, 165-172.
• 4.Young, G.P. & Gibson, P.R. (1994) Butyrate and the colorectal cancer cell; in Short-chain Fatty Acids (Cummings, J., Binder, H.J. & Soergel, K., eds.) pp. 148-160, Kluwer Academic Publishers, Boston.
• 5.Gibson, P.R., Rosella, O., Wilson, A.J., Mariadason, J.M., Rickard, K., Byron, K. & Barkla, D.H. (1999) Colonic epithelial cell activation and the paradoxical effects of butyrate. Carcinogenesis. 20, 539-544.
• 6.Scheppach, W.M., Bartram, P., Richter, A., Richter, F., Liepold, H., Dusel, G., Hofstetter, G., Ruthlein, J. & Kasper, H. (1992) Effect of short-chain fatty acids on the human colonic mucosa in vitro. J. Parenter. Enteral. Nutr. 16, 43-48.
• 7.Kripke, S.A., Fox, A.D., Berman, J.M., Settle, R.G. & Rombeau, J.L. (1989) Stimulation of intestinal mucosal growth with intracolonic infusion of short-chain fatty acids. J. Parenter. Enteral. Nutr. 13, 109-116.
• 8.Luciano, L., Hass, R., Busche, R., von Engelhardt, W. & Reale, E. (1996) Withdrawal of butyrate from the colonic mucosa triggers "mass apoptosis" primarily in the G0/G1 phase of the cell cycle. Cell Tissue Res. 286, 81-92.
• 9.Basson, M.D., Turowski, G.A., Rashid, Z., Hong, F. & Madri, J.A. (1996) Regulation of human colonic cell line proliferation and phenotype by sodium butyrate. Dig. Dis. Sci. 41, 1989-1993.
• 10.Gamet, L., Daviaud, D., Denis-Pouxviel, C., Remesy, C. & Murat, J.C. (1992) Effects of short-chain fatty acids on growth and differentiation of the human colon-cancer cell line HT29. Int. J. Cancer. 52, 286-289.
• 11.Heerdt, B.G., Houston, M.A. & Augenlicht, L.H. (1994) Potentiation by specific short- chain fatty acids of differentiation and apoptosis in human colonic carcinoma cell lines. Cancer Res. 54, 3288-3293.
• 12.McIntyre, A., Young, G.P., Taranto, T., Gibson, P.R. & Ward, P. (1993) Fermentation products of dietary fiber and protection against large bowel cancer in a rat model. Gut. 34, 386-391.
• 13.D'Argerio, G., Cosenza, V., Delle Cave, M., Iovino, P., DellaValle, N., Lombardi, G. & Mazzacca, G. (1996) Butyrate enemas in experimental colitis and protection against large bowel cancer in a rat model. Gastroenterology. 110, 1727-1734.
• 14. Medina, V., Afonso, J.T., Alverez-Arguelles, H., Hernandez, C. & Gonzales, F. (1998) Sodium butyrate inhibits carcinoma development in a 1,2-dimethylhydrazine-induced rat colon cancer. J. Ent. Parent. Nutr. 22, 14-17.
• 15.Scheppach, W.M., Sommer, H., Kirchner, T., Paganelli, G.M., Bartram, P., Christi, S., Richter, F., Dusel, G. & Kasper, H. (1992) Effect of butyrate enemas on the colonic mucosa in distal ulcerative colitis. Gastroenterology. 103, 51-56.
• 16.Hague, A., Manning, A.M., Hanlon, K.A., Huschtscha, L.I., Hart, D. & Paraskeva, C. (1993) Sodium butyrate induces apoptosis in human colonic tumour cell lines in a p53-independent pathway - implications for the possible role of dietary fibre in the prevention of large bowel cancer. Int. J. Cancer. 55, 498-505. Dzierzewicz Z, Orchel A
• 17.Singh, B., Halestrap, A.P. & Paraskeva, C. (1997) Butyrate can act as a stimulator of growth or inducer of apoptosis in human colonic epithelial cell lines depending on the presence of alternative energy sources. Carcinogenesis. 18, 1265-1270.
• 18.Dzierzewicz, Z., Orchel, A., Swierczek-Zieba, G., Latocha, M., Cwalina, B. & Wilczok, T. (2001) Cultivation of normal colonic epithelial cells. Gastroenterol. Pol. 8, 59-65.
• 19.Fusunyan, R.D., Quinn, J.J., Ohno, Y., McDermott, R.P. & Sanderson, I.R. (1998) Butyrate enhances interleukin (IL)- 8 secretion by intestinal epithelial cells in response to IL-1beta and lipopolysaccharide. Pediatr. Res. 43, 84-90.
• 20.Litvak, D.A., Evers, M.B., Hwang, K.O., Hellmich, M.R., Ko, T.C. & Townsend, C M. (1998) Butyrate-induced differentiation of Caco-2 cells is associated with apoptosis and early induction of p21Waf1/Cip1 and p27Kip1. Surgery. 124, 161-170.
• 21.Mariadason, J.M., Velcich, A., Wilson, A.J., Augenlicht, L.H. & Gibson, P.R. (2001) Resistance to butyrate-induced cell differentiation and apoptosis during spontaneous Caco-2 cell differentiation. Gastroenterology. 120, 889-899.
• 22.Stadnyk, A.W. (1994) Cytokine production by epithelial cells. FASEB J. 8, 1041-1047.
• 23.Eckmann, L., Kagnoff, M.F. & Fierer, J. (1993) Epithelial cells secrete the chemokine interleukin-8 in response to bacterial entry. Infect. Immun. 61, 4569-4574.