The influence of semen cryopreservation on the release of some enzymes from Siberian sturgeon [Acipenser baerii] and sterlet [Acipenser ruthenus] spermatozoa

• Autorzy: Sarosiek B , Ciereszko A , Rzemieniecki A , Domagala J , Glogowski J
• Języki publikacji: EN

Abstrakty

EN

The milt of individual males of Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthenus) was frozen without cryoprotector (at -79°C) or cryopreserved with methanol as the cryoprotector. The activity of arylsulfatase (AS), acid phosphatase (AcP), β-N-acetylglucosaminidase (NAGase), and protein concentration was determined. The protein concentration and enzymatic activities in supernatant obtained after cryopreservation were higher than in milt plasma, but they were lower than that in the material obtained after freezing at -79°C. The protein and enzymatic leakage of sterlet spermatozoa was statistically higher in supernatants that had been frozen at-79°C than in those that had undergone cryopreservation. Differences in the protein and AS leakage the Siberian sturgeon supernatants were also observed.

PL

Nasienie pobrane od 19 osobników jesiotra syberyjskiego (Acipenser baerii) i 10 - sterleta (A. ruthenus) zamrożono w -79°C bez obecności krioprotektora oraz poddano kriokonserwacji z metanolem jako krioprotektorem. Koncentrację oraz ruchliwość użytego w eksperymencie nasienia zamieszczono w tabeli 1. W świeżej plazmie nasienia oraz supernatantach uzyskanych po odwirowaniu zamrożonego materiału oznaczono zawartość białka oraz aktywność arylosulfatazy (AS), kwaśnej fosfatazy (AcP) i β-N-acetyloglukozoaminidazy (NAGase) (tabela 2). Oznaczone parametry w supernatancie uzyskanym po kriokonserwacji były statystycznie istotnie wyższe niż w plazmie nasienia i jednocześnie niższe niż supernatancie po mrożeniu bez obecności metanolu (tabela 2). Wykazano również istnienie korelacji pomiędzy „wyciekiem" arylosulfatazy i koncentracją plemników w materiale uzyskanym po mrożeniu w -79°C (rys. 1). W supernatancie po kriokonserwacji nie obserwowano istnienia takiej zależności (rys. 2).

Słowa kluczowe

EN

Acipenser ruthenus; semen; sterlet; male; Siberian sturgeon; Acipenser baeri; cryopreservation

• Wydawca: -
• Czasopismo: Archives of Polish Fisheries
• Rocznik: 2004
• Tom: 12
• Numer: 1
• Opis fizyczny: p.13-21,fig.,ref.

Twórcy

autor

Sarosiek B

• Molecular Andrology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Bydgoska 1/8, 10-243 Olsztyn, Poland

autor

Ciereszko A

• Semen Biology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences Olgsztyn, Poland

autor

Rzemieniecki A

• Department of General Zoology, Szczecin University, Poland

autor

Domagala J

• Department of General Zoology, Szczecin University, Poland

autor

Glogowski J

• Division of Evolutionary Genetics, University of Warmia and Mazury in Olsztyn, Poland
• Molecular Andrology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Bydgoska 1/8, 10-243 Olsztyn, Poland

Bibliografia

• Babiak I., Głogowski J., Brzuska E., Szumieć J., Adamek J. 1997a - Cryopreservation of sperm of common carp, Cyprinus carpio L. - Aquacult. Res. 28: 567-571.
• Babiak I., Glogowski J., Luczynski M. J., Luczynski M. 1997b - Effect of individual male variability on cryopreservation of northern pike Esox lucius L., sperm - Aquacult. Res. 28:191-197.
• Beamesderfer R.C.P., Farr R.A. 1997 - Alternatives for the protection and restoration of sturgeons and their habitat - Environ. Biol. Fishes 48: 407-417.
• Billard R., Cosson J., Crim L.W., Suquet M. 1995 - Sperm physiology and quality - In: Bromage N.R., Roberts R.J. (Eds.), Broodstock Management and Egg and larval Quality. Blackwell, Oxford, pp. 25-52.
• Brandon Jr. C. I, Srivastava P. N., Heusner G. L., Fayrer-Hosken R. A. 1997 - Extraction and quantification of acrosin, β-N-acetylglucosoaminidase, and arylsulfatase A from equine ejaculated spermatozoa - J. Exp. Zool. 279:301-308.
• Carmona E., Weerrachatyanukul W., Soboloff T., Fluharthy A. L., White D., Promdee L., Ekker M., Berger T., Buhr M., Tanphaichitr N. 2002 - Arylsulfatase A is present on the pig sperm surface and is involved in sperm-zona pellucida binding - Dev. Biol. 247(1): 182-96.
• Cherr G. N., Clark W. H. 1984 - An acrosome reaction in sperm from the white sturgeon Acipenser transmonatus - J. Exp. Zool. 232:129-139.
• Ciereszko A., Dąbrowski K. 1993 - Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using a spectrophotometric technique - Aquaculture 109:1292-1305.
• Ciereszko A., Dąbrowski K., Ochkur S. I. 1996 - Characterization of acrosin-like activity of lake sturgeon (Acipenser fulvescens) spermatozoa - Mol. Reprod. Dev. 45: 72-77.
• Gadella B.M., Colenbrander B., Van Golde L.M.G., Lopes-Cardozo M. 1993 - Boar seminal vesicle secrete arylsulfatases into seminal plasma - evidence that desulfation of seminolipid occurs only after ejaculation - Biol. Reprod. 48: 483-489.
• Glogowski J., Babiak I., Goryczko K., Dobosz S. 1996 - Activity of aspartate aminotransferase and acid phosphatase in cryopreserved trout semen - Reprod. Fert. Dev. 8:1179-1184.
• Glogowski J., Babiak I., Luczynski M. J., Luczynski M. 1997a - Factors affecting cryopreservation efficiency and enzyme activity in northern pike Esox lucius, sperm - J. Appl. Aquacult. 7(4): 53-67.
• Glogowski J., Babiak I., Kucharczyk D., Luczynski M. 1997b - The effect of individual male variability on cryopreservation of bream (Abramis brama L.) sperm - Pol. Arch. Hydrobiol. 44: 281-285.
• Glogowski J., Babiak I., Kucharczyk D., Luczynski M., Piros B. 1999 - Some properties of bream Abramis brama L. sperm and its cryopreservation - Aquacult. Res. 30: 765-772.
• Glogowski J., Kolman R., Szczepkowski M., Horváth Á., Urbányi B., Sieczyński P., Rzemieniecki A., Domagała J., Demianowicz W., Kowalski R., Ciereszko A. 2002 - Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol - Aquaculture 211: 367-373.
• Horvath L., Szabo T., Burke J. 1997 - Hatchery testing of GnRH analogue-containing pellets on ovulation in four cyprinid species - Pol. Arch. Hydrobiol. 44: 221-226.
• Jamieson B. G. M. 1991 - Fish evolution and systematics: evidence from spermatozoa - Cambridge Univ. Press, Cambridge, New York, Port Chester, Melbourne, Sydney.
• Jauhiainen A., Vanha-Perttula T. 1986 - β-N-acetylglucosaminidase in the reproductive organs and seminal plasma of the bull - J. Reprod. Fertil. 76: 239-250.
• Jähnichen H., Warnecke W., Trölsch E., Kohlmann K., Bergler H., Pluta H. J. 1999 - Motility and fertilizing capability of cryopreserved Acipenser rulhenus L. sperm - J. Appl. Ichthyol. 15:204-206.
• Lowry O. H., Rosebrough N. J., Farr A. L., Raudall R. J. 1951 - Protein measurement with folin-phenol reagent - J. Biol. Chem. 193:265-275.
• Miller D. J., Gong X., Shur B. D. 1993 - Sperm require β-N-acetylglucosaminidase to penetrate through the egg zona pellucida - Development 118:1279-1289.
• Tsvetkova L. I., Cosson J., Linhart O., Billard R. 1996 - Motility and fertilizing capacity of fresh and frozen-thawed spermatozoa in sturgeons Acipenser baeri and A. ruthenus - J. Appl. Ichthyol. 12:107-112.
• Watson P. F. 2000 - The causes of reduced fertility with cryopreserved semen - Anim. Reprod. Sei. 60-61:481-492.