Apoptosis and clonogenic survival in three tumour cell lines exposed to gamma rays or chemical genotoxic agents

• Autorzy: Kumala S , Niemiec P. , Widel M. , Hancock R. , Rzeszowska-Wolny J.
• Języki publikacji: EN

Abstrakty

EN

We compared the extent to which apoptosis is induced and clonogenicity reduced in three tumour cell lines - the human melanoma Me45 and promyelocytic leukaemia HL-60, and the rat rhabdomyosarcoma R1 - after exposure to the anticancer drugs etoposide and cis-platinum or to gamma radiation; each induces different types of DNA damage. Cells which readily underwent apoptosis did not necessarily show a correlated loss of clonogenicity; for example, Me45 cells showed the highest sensitivity to all three agents in clonogenic assays but much lower levels of apoptotic cells than R1 or HL-60 cells. These results show that the efficiency of the eradication of clonogenic cells by genotoxic agents does not solely depend on the induction of apoptotic processes, and suggest that the induction of apoptosis and suppression of clonogenicity are independent processes.

Słowa kluczowe

EN

cell line; tumour cell; therapy; genotoxic agent; cytotoxic agent; gamma-ray; tumour; apoptosis; micronucleus; survival

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 3
• Opis fizyczny: p.655-665,fig.

Twórcy

autor

Kumala S

• Center of Oncology, Wybrzeze AK 15, 44-100 Gliwice, Poland

autor

Niemiec P.

autor

Widel M.

autor

Hancock R.

autor

Rzeszowska-Wolny J.

Bibliografia

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