Cloning and expression of the genes coding for tube associated proteins of bacteriophage T4

• Autorzy: Nieradko J.
• Języki publikacji: EN

Abstrakty

EN

Genes 29,48 and 54 of bacteriophage T4, coding for specific tube associated proteins, were cloned to the expression vector pT7-5. The molecular mass of the products of these genes was estimated to be 64,39 and 36 kDa, respectively. The examined genes are cotranscribed with genes 51,27 and 28 from the same DNA strand and a common late promoter sequence located downstream of gene 51.

Słowa kluczowe

EN

tube associated protein; promoter; cloning; gene coding; baseplate gene; DNA; bacteriophage T4

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 2
• Opis fizyczny: p.275-279,fig.

Twórcy

autor

Nieradko J.

• University of Gdansk, Kladki 24, 80-822 Gdansk, Poland

Bibliografia

• 1. Goldberg, E. (1983) Bacteriophage T4> pp. 32-39, American Society for Microbiology, Washington DC.
• 2. Duda, L., Gingery, M. & Eiserling, F.A. (1986) Virology 151,296-314.
• 3. Kikuchi, K. & King, J. (1975) }. Mol. Biol. 99, 645-672.
• 4. Kikuchi, K. & King, J. (1975) }. Mol. Biol 99, 673-694.
• 5. Kikuchi, K. & King, ). (1975) J. Mol Biol. 99, 695-716.
• 6. Kutter, E. & Snyder, L. (1983) Bacteriophage T4; pp. 56-58, American Society for Microbiology, Washington DC.
• 7. Tabor, S. & Richardson, C. (1985). Proc. Natl Acad. Sci. U.S.A. 82,1074-1078.
• 8. Maniatis, T., Fritsch, E.F. & Sambrook, J. (1982) Molecular cloning. A laboratory manual Cold Spring Harbor Laboratory Press, New York.
• 9. Nieradko, J. & Szewczyk, B. (1990) Ada Microbiol Polon. 39,13-22.
• 10. Nieradko, J. & Podgorska, B. (1993) Acta Biochim. Polon. 38,313-319.
• 11. Scftichtholtz, B., Nieradko, J., Gajek, M. & Szewczyk, B. (1991) Acta Biochim. Polon. 38, 311-319.
• 12. Laemmli, K. (1970) Nature (London) 227, 680-685.
• 13. Kutter, B., Gutman, B., Mosig, G. & Ruger, W. (1990) Genetic Map; pp. 124-261, Cold Spring Laboratory Press Harbor, Cold Spring Harbor, N.Y.
• 14. Scarlato, V., Storlazi, A. Gargano, S. & Cascino, A. (1989) Virology 171,475-483.
• 15. Ishimoto, K., Ishimoto, S., Cascino, A., Cpilaro, S. & Eiserling, F. (1988) Virology 164,81-90.