Zastosowanie metody do wykrywania wybranych genow Mycoplasma pneumoniae

• Autorzy: Rastawicki W. , Jagielski M. , Gierczynski R.
• Języki publikacji: PL

Abstrakty

PL

Wystandaryzowano technikę łańcuchowej reakcji polimerazy (PCR) do wykrywania genów M. pneumoniae kodujących białko PI, pod jednostkę 16S rRNA i czynnik ekongacyjny Tu. Stwierdzono, że PCR jest techniką umożliwiającą wykrycie poszukiwanych genów nawet wtedy, gdy w 1 ml hodowli znajduje się od 10(2) do 10(4) komórek M. pneumoniae.

EN

The aim of this study was standardization of PCR for the detection of gene encoding the PI protein, 16S rRNA and elongation factor Tu of M. pneumoniae. A total of 13 strains of M. pneumoniae, 28 strains of other mycoplasmas and 14 strains of different bacteria causing respiratory tract infections were tested. In all of tested M. pneumoniae strains the presence of the sought genes was confirmed. The specificity of DNA was confirmed by the restriction endonuclease analysis with enzymes Hind III, Alu I and Hha I. With none of primers specific for the M. pneumoniae genes amplification of DNA from other bacteria was noted. The PCR method with the selected primers allowed to detect from 10(2) to 10(4) cfu M. pneumoniae/ml suspended in broth. The obtained results indicate that the PCR method can be used for detection of M. pneumoniae genes. A very good sensitivity and specificity predestine PCR as a potential quick diagnostic method for identification of M. pneumoniae in clinical specimens.

Słowa kluczowe

PL

infekcja bakteryjna; rRNA; bialko P1; czynnik elongacyjny Tu; szczepy bakteryjne; Mycoplasma pneumoniae; lancuchowa reakcja polimerazy; oznaczanie; mikrobiologia; geny; diagnostyka laboratoryjna

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2000
• Tom: 52
• Numer: 1
• Opis fizyczny: s.67-74,fot.,bibliogr.

Twórcy

autor

Rastawicki W.

• Panstwowy Zaklad Higieny, 00-791 Warszawa, ul.Chocimska 24

autor

Jagielski M.

autor

Gierczynski R.

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