A rapid method of agarose gel electrophoresis for separation of different conformational forms of DNA

• Autorzy: Ciesielska E. , Szmigiero L.
• Języki publikacji: EN

Abstrakty

EN

A fast two step method of agarose gel electrophoresis for separation of different conformational forms of DNA is described. In the first step the gel is run in the buffer without ethidium bromide and then the gel is stained with that dye. After staining the second step of electrophoresis is performed in the buffer without ethidium bromide. This two step procedure allows one to receive very good resolution between bands corresponding to relaxed, supercoiled, open circular, and linear forms of DNA.

Słowa kluczowe

EN

DNA electrophoresis; agarose; ethidium bromide; DNA conformation; gel electrophoresis

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1996
• Tom: 01
• Numer: 4
• Opis fizyczny: p.373-376,fig.

Twórcy

autor

Ciesielska E.

• Medical University of Lodz, Lodz, Poland

autor

Szmigiero L.

Bibliografia

• 1. Sambrook, J., Fritsch, E.F. and Maniatis T. in Molecular Cloning a Laboratory Manual, (Ford N., Ed.),, Cold Spring Harbor Laboratory Press, New York, 1989, 6.0-6.62.
• 2. Hertzberg, R.P., M.J. Caranfa and S.M. Hecht. Biochemistry 28 (1989) 4629-4638.
• 3. Osheroff, N. Biochemistry 26 (1987) 6402-6406.