Purification and some properties of a novel dsRNA degrading nuclease bound to rye germ ribosomes

• Autorzy: Siwecka M. A.
• Języki publikacji: EN

Abstrakty

EN

A two-step procedure including affinity chromatography for purification of rye germ ribosomal nuclease that degrades double-stranded RNA from a virus of Penicillium chrysogenum and the poly(I).poIy(C) complex was developed. The specific activity towards poly(I).poly(C) of the obtained nuclease preparations was 30 times as high as that of ribosomes. The recovery of activity was 3.4% when the Octyl-Sepharose column was used, and 2.0% in the case of the Phenyl-Sepharose column. On polyacrylamide/SDS gel electrophoresis the nu­clease was resolved into two proteins of molecular mass 62 kDa and 57 kDa, respectively. 2-Mercaptoethanol and Mn2+ stimulated the activity of the puri­fied enzyme. Glycerol (20%-50% concentration) stabilized enzyme. In addition to activity towards dsRNA and ssRNA the enzyme cleaves native and denatured DNA. It is suggested that this type of a nuclease takes part in regulation of the mRNA level in cytoplasm.

Słowa kluczowe

EN

affinity chromatography; RNA; virus; rye; ribosome; Secale cereale; mRNA; germ; nuclease; Penicillium chrysogenum; purification; cytoplasm

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1997
• Tom: 44
• Numer: 1
• Opis fizyczny: p.61-68,fig.

Twórcy

autor

Siwecka M. A.

• Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland

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