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Tytuł artykułu

Przydatnosc metody PCR i jej modyfikacji do diagnozowania inwazji pasozytniczych u przezuwaczy

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Treść / Zawartość

Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
Sensitivity and specificity are the two most important criteria that define the quality of a diagnostic technique. DNA probes and PCR-based techniques may simplify the diagnosis of parasitic infections. PCR is a powerful diagnostics tool. The method enables detection of even a very small amount of DNA. An extreme sensitivity of the PCR, being a major advantage of the method is also a cause of potentially false positive results. To achieve reliable diagnostic results several modifications have been introduced to the classic PCR procedure. PCR and PCR-based techniques are currently increasingly used for detection of parasitic infections, to differentiate closely related species which are difficult to be recognised with traditional methods, for estimation of parasite burdens, and for the detection of drug resistant strains.

Wydawca

-

Rocznik

Tom

46

Numer

3

Opis fizyczny

s.295-304,rys.,tab.,bibliogr.

Twórcy

  • Instytut Parzytologii PAN, ul.Twarda 51/55, 00-818 Warszawa

Bibliografia

  • ATKINSON R., HARPER P.A.W., REICHEL M.P., ELLIS J.T. 2000. Progress in the serodiagnosis of Neospora caninum infections of cattle. Parasitol. Today 16: 110- 114.
  • ALLES A.J., WALDRON M.A., SIERRA L.S., MATTIA A.R. 1995. Prospective comparison of direct immunofluorescence and conventional staining method for detection of Giardia and Cryptosporidium in human fecal specimens. J. Clin. Microbiol. 33: 1632-1634.
  • BJORKMAN C., UGGLA A. 1999. Serological diagnosis of Neospora caninum infection. Int. J. Parasit. 29: 1497-1507.
  • DUBEY J.P. 1999. Recent advances in Neospora and neosporosis. Vet. Parasitol. 84: 349-367.
  • ELLIS J.T. AMOYAL G., RYCE C. 1998. Comparison of the large subunit ribosomal DNA of Neospora caninum and Toxoplasma and development of a new genetic marker for their differentiation based on the D2 domain. Mol. Cell. Probes 12: 1-13.
  • ELLIS J.T., MCMILLAN D., RYCE C., PAYNE S., ATKINSON R., HARPER P.A.W. 1999. Development of a single tube nested polymerase chain reaction assay for the detection of Neospora caninum DNA. Int. J. Parasit. 29: 1589-1596.
  • FELEISEN R.S.J. 1998. Comparative genetic analysis of Tritrichomonadid protozoa by the Random Amplified Polymorphic DNA technique. Parasitol. Res. 84: 153-156.
  • FELEISEN R.S.J., LAMBELET N., BACHMANN P., NICOLET J., MULLER N., GOTTSTEIN B. 1998. Detection of Tritrichomonas faetus by PCR and DNA enzyme immunoassay based on rRNA gene unit sequences. J. Clin. Microbiol. 36: 513-519.
  • GASSER R.B. 1999. PCR-based technology in veterinary parasitology. Vet. Parasitol. 84: 229-258.
  • JOHANSON D. W., PIENIĄŻEK N.J., GRIFFIN D. W. 1995. Development of a PCR protocol for sensitive detection of Cryptosporidium oocyst in water samples. Appl. & Environ. Microbiol. 61: 3849-3855.
  • JENKINS M., TROUT J., FAYER R. 1997. A semiquantitative method for measuring Cryptosporidium parvum, infection using polymerase chain reaction. J. Microbiol. Meth. 28: 99-107.
  • KATAKURA K., LUBINGA C., CHITANBO H., TADA Y. 1997. Detection of Trypanosoma congolense and T. brucei subspecies in cattle in Zambia by polymerase chain reaction from blood collected on filter paper. Parasitol. Res. 83: 241-245.
  • KAUFMAN H., YAMAGE M., RODITI I. 1996. Discriminatioą of Neospora caninum from Toxoplasma gondii and other apicomplexan parasites by hybridization and PCR. Mol. Cell Probes 10: 289-297.
  • LALLY N.C., JENKINS M.C., DUBEY J.P. 1996. Development of a polymerase chain reaction assay for the diagnosis of neosporosis using the Neospora caninum 14-3-3 gene. Molec. Biochem. Parasitol. 75: 169-178.
  • LENG X., MOSIER D.A., OBERST R.D. 1996. Simplified method for recovery and PCR detection of Cryptosporidium DNA from bovine feces. Appl. Environment. Microbiol. 62: 643-647.
  • LIDDELL S., JENKINS M.C., DUBEY J.P. 1999. A competitive PCR assay for quantitative detection of Neospora caninum. Int. J. Parasitol. 29: 1583-1587.
  • MCALLISTER M.M., DUBEY J.P., LINDSAY D.S., JOLLEY W.R., WILLIS R.A., MCGUIRE A.M. 1999. Dogs are definitive hosts of Neospora caninum. Int. J. Parasit. 28: 1473-1478.
  • MEHLHORN H., HEYDORN A.O. 2000. Neospora caninum: Is it really different from Hammondia heydorni or is it a strain of Toxoplasma gondii? An opinion. Parasitol. Res. 86: 169-178.
  • MIESZCZANEK J. WĘDRYCHOWICZ H. 1999. Differentiation of two hookworm species using PCR. Acta Parasit. 44: 54-56.
  • MORGAN U.M., PALLANT L., DWYER B., FORBES D.A., THOMPSON R.C.A. 1998. Comparison of PCR and microscopy for the detection of Cryptosporidium parvum in human faecal specimens - Clinical trial. J. Clin. Microbiol. 36: 995-998.
  • MORGAN U.M., THOMSON R.C.A. 1998. PCR detection of Cryptosporidium: the way forward? Parasitol. Today 14: 241-145.
  • MONTEIRO L., BONNEMAISON D., VERKIS A., PETRY K.G., BONNET J., VIDAL R., CARBITA J., MEGRAUD F. 1997. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J. Clin. Microbiol. 35: 995-998.
  • MUGRIDGE N.B., MORRISON D.A., HECKEROTH A.R., JOHSON A.M., TENTER A.M. 1999. Phylogenetic analysis based on full-length large submit ribosomal RNA gene sequence comparison reveals that Neospora caninum is more closely related to Hammondia heydorni than to Toxoplasma gondii. Intern. J. Parasitol. 29: 1545-1556.
  • PETERS M., WAGNER F., SCHARES G. 2000. Canine neosporosis: clinical and pathological findings and first isolation of Neospora caninum in Germany. Parasitol. Res. 86: 1-7.
  • SCHNIEDER T., HEISE M., EPE C. 1999. Genus-specific PCR for the differentiation of eggs or larvae from gastrointestinal nematodes of ruminants. Parasitol. Res. 85: 895-898.
  • UWATOKOK., SUNAIRI M., YAMAMOTO A., NAKAJIMA M., YAMAURA K. 1996. Rapid and efficient method to eliminate substances inhibitory to the polymerase chain reaction from animal faecal samples. Vet. Microbiol. 52: 73-79.
  • WEBSTER K.A., SMITH H.V., GILES M. 1996. Detection of Cryptosporidium parvum in faeces: comparison of conventional coproscopical methods and the polymerase chain reaction. Vet. Parasitol. 61: 5-13.
  • WILLIAMS J.G.K., KUBELIK A.R., LIVAK K.J., RAFALSKI J.A., TINGEY S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuci. Acids Res. 18: 6531-6535.
  • YULE A, SKIRROW S.Z., STAATS J., BONDURANT R.H. 1989. Development and preliminary assessment of a polyclonal antibody-based enzyme immunoassay for the detection of Tritrichomonas foetus antigen in breeding cattle. Vet. Parasitol. 31: 115-123.
  • ZINDROU S., LE D.D., PHAM T.X., NGUYEN P.D., NGUYEND. D.S., SKOLD 0., SWEDBERG G. 1996. Rapid detection of pyrimethamine susceptibility of Plasmodium falciparum by restriction endonuclease digestion of dihydrofolate reductase gene. Am. J. Trop. Med. Hyg. 54: 185-188.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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