The immunoreactivity of c-Fos, NGF and its receptor TrkA after open-field exposure in the central and medial nuclei of the rat amygdala

• Autorzy: Badowska-Szalewska E , Ludkiewicz B. , Domaradzka-Pytel B. , Dziewiatkowski J. , Spodnik J.H. , Morys J.
• Języki publikacji: EN

Abstrakty

EN

The amygdala is a critical component of the neuroanatomical stress circuit. It plays a role in the generation of responses to emotional stimuli. The central (CeA) and medial (MeA) amygdaloid nuclei are implicated in activation of the hypothalamic-pituitary-adrenocortical (HPA) axis. The immunoreactivity (-ir) of c-Fos, NGF and its receptor, TrkA, following acute and chronic open-field stress were studied in the CeA and MeA nuclei of the amygdala. The material consisted of 21 male adult rats divided into three groups: non-stressed (control) animals, rats exposed to acute (once only lasting 15 min) and chronic (15 min daily over 21 days) aversive stimulation (open-field exposure). The brains were stained with the use of immunohistochemical methods for c-Fos, NGF or TrkA. In the control rats c-Fos-, TrkA- and NGF-ir cells were observed in the nuclei studied, but the quantity varied, being moderate or high (immunoreactive to TrkA and NGF) or low (immunoreactive to c-Fos). In the animals exposed to acute open-field stress the number of c-Fos-ir, NGF-ir and TrkA-ir cells in the nuclei under examination was differentiated but higher than that in the control animals. In the animals exposed to chronic open-field stress the number of c-Fos-ir cells in the nuclei studied was similar and was smaller than those in animals exposed to acute stress. The number of TrkA-ir neurons was also lower in comparison to that in animals exposed to acute stress. However, no significant differences in the number of NGF-ir cells were observed between the groups exposed to acute and chronic stress. Diverse expression of c-Fos protein following both acute and chronic stress stimulation may prove the functional heterogeneity of the amygdaloid nuclei investigated. The decrease observed in both c-Fos- and TrkA-ir in MeA (only TrkA in CeA) of animals exposed to chronic stress may indicate the phenomenon of habituation.

Słowa kluczowe

EN

immunoreactivity; Trk A receptor; open field exposure; stress; central nucleus; medial nucleus; rat; amygdala; neurotrophin

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2006
• Tom: 65
• Numer: 2
• Opis fizyczny: p.145-151,fig.,ref.

Twórcy

autor

Badowska-Szalewska E

• Medical University of Gdansk, Debinki 1, 80-211 Gdansk, Poland

autor

Ludkiewicz B.

autor

Domaradzka-Pytel B.

autor

Dziewiatkowski J.

autor

Spodnik J.H.

autor

Morys J.

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