Augmenting in vitro shoot multiplication with growth regulators and light conditions in Musa acuminata cv. Dwarf Cavendish

• Autorzy: Rout G. R. , Samantaray S. , Das P.
• Języki publikacji: EN

Abstrakty

EN

An efficient protocol was developed for in vitro mass propagation of the commercial cultivar Musa acuminata cv. Dwarf Cavendish through shoot apices culture. Shoot apices with 2-3 pairs of leaf primordia were induced from sliced rhizome explants on Murashige and Skoog (1962) medium supplemented with 6.0 mg/l BA, 150 mg/l Ads and 3% (w/v) sucrose. Multiple shoots were induced from meristematic domes on the same medium. Addition of 1.0-1.5 mg/l IAA to the culture medium and incubation in continuous light (24 h) increased the yield of multiple shoots. The high multiplication coefficient was maintained stable up to the 5th subculture; thereafter it declined. Rooting was readily achieved upon transferring the shoots onto half-strength MS medium supplemented with 500 mg/l activated charcoal and 2% (w/v) sucrose. Micropropagated plantlets were hardened in a polyethylene house and successfully established in soil. There was no morphological variation among the micropropagated plants.

Słowa kluczowe

EN

fruit crop; micropropagation; Musa; banana plant; commercial cultivar; in vitro; Musa acuminata; plantain; growth regulator; light condition; banana; shoot; Dwarf Cavendish cultivar

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2001
• Tom: 43
• Opis fizyczny: p.15-21,fig.,ref.

Twórcy

autor

Rout G. R.

• Regional Plant Resource Centre, Bhubaneswar 751015, India

autor

Samantaray S.

autor

Das P.

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