Unidirectional orientation of twelve expressed tagged sites within 4o kb of human chromosomal region 22q13.1

• Autorzy: Pusch C , Wang Z. , Roe B. , Blin N.
• Języki publikacji: EN

Abstrakty

EN

The single copy sequence D22S16 from human chromosomal region 22q13.1 that carries a putative conserved gene, was used to probe a chromosome 22-specific cosmid library. Genomic sequencing of one positive, 40 kb long cosmid (C1155) revealed a hereto unmapped gene (a subunit of DNA-dependent RNA polymerase II, POLR2F), a SOX9-related sequence and 12 expressed sequence tags. Although not parts of one consecutive gene, all 12 ESTs and, in addition, the polymerase gene are oriented in the same transcriptional direction within the genomic sequence represented by cosmid C1155.

Słowa kluczowe

EN

RNA; human chromosome; chromosome; hybridization; genetic change; linear map; polymerase chain reaction; DNA; cosmid; cDNA; transcriptional orientation

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1998
• Tom: 39
• Numer: 2
• Opis fizyczny: p.199-204,fig.

Twórcy

autor

Pusch C

• University of Tubingen, Wilhelmstr.27, 72074 Tubingen, Germany

autor

Wang Z.

autor

Roe B.

autor

Blin N.

Bibliografia

• Acker J., Mattei М.-G., Wintzerith M., Roeckel N., Depetris D., Vigneron M., Kedinger C. (1994a). Chromosomal localization of human RNA polymerase II subunit genes. Genomics 20: 496-499.
• Acker J., Wintzerith M., Vigneron M., Kedinger C. (1994b). A 14.4 КDa acidic subunit of human RNA polymerase II with a putative leucine-zipper. DNA Seq. 4: 329-331.
• Bell C., Budarf M., Nieuwenhuijsen B., Baronski B., Buetow K., Campbell K., Colbert A., Collins J., Daly M., Desjardins P., Dezwaan T., Eckman B., Foote S., Hart K., Hiester K., van Неt Hoog M., Hopper E., Kaufman A., McDermid H., Overton G., Reeve M., Searls D., Stein L., Valmiki V., Watson E., Williams S., Winston R., Nussbaum R., Lander E., Fischbeck K., Emanuel B., Hudson T. (1995). Integration of physical, breakpoint and genetic maps of chromosome 22. Hum. Mol. Genet. 4: 59-69.
• Blin N., Müllenbach R., Suijkerbuijk R., Herzog R., Meese E. (1993). Cosmid mapping and locus linkage within the human chromosomal region 22q13.1. Cancer Genet. Cytogenet. 70: 108-111.
• Bodenteich A., Chissoe S., Wang Y.F., Roe B.A. (1993). Shotgun cloning as the strategy of choice to generate templates for high-throughput dideoxynucleotide sequencing. In: Automated DNA Sequencing and Analysis Techniques (J.C. Venter, ed.). Academic Press, London: 42-50.
• Collins J.E., Cole C.G., Smink L., Garrett C., Leversha M., Soderlund C., Maslen G., Everett L., Rice K., Coffey A., Gregory S., Gwilliam R., Dunham A., Davies A., Hassock S., Todd C., Lehrach H., Hulsebos J., Weissenbach J., Morrow B., Kucherlapati R., Wadey R., Scambler P., Kim U.J. (1995). A high density YAC contig map of human chromosome 22. Nature 377: 369-379.
• Gaypay G., Morissette J., Viginal A., Dib C., Fizames C., Millasseau P., Marc S., Bernardi G., Lathrop M., Weissenbach J. (1994). The 1993-94 Genethon human genetic linkage map. Nature Genet. 7: 246-334.
• Gough N.M. (1988). Rapid and quantitative preparation of cytoplasmic RNA from small number of cells. Anal. Biochem. 173: 93.
• Göttert E., Metzdorf R., Färber U., Blin N. (1989). Regional localization and molecular characterization of a DNA sequence on the long arm of chromosome 22. Hum. Genet. 81: 385-387.
• Nizetic D., Zehenter G., Monaco A.P., Gellen L., Young B.D., Lehrach H. (1991). Construction, arraying and high-density screening of large insert libraries of human chromosomes X and 21: Their potential use as reference libraries. Proc. Natl. Acad. Sci. USA 88: 3233-3237.
• Pusch C., Hustert E., Pfeifer D., Südbeck P., Roe B., Wang Z., Balling R., Blin N., Scherer G. (1998). The SOX10/sox10 gene from human and mouse: sequence, expression and transactivation by the encoded HGM domain transcription factor. Hum. Genet., in press.
• Pusch C., Wang Z., Roe B., Blin N. (1996). Genomic structure of RNA polymerase II small subunit (hRPB14.4) and mapping to 22q13.1 by sequence identity. Genomics 34: 440-442.
• Riley B., Williamson R., Vergnaud G. (1994). The EUROGEM map of human chromosome 22. Europ. J. Hum. Genet. 2: 246-247.
• Schmitt H., Kim U.J., Slepak T., Blin N., Simon M., Shizuya H. (1996). Framework for a physical map of the human 22q13-region using Bacterial Artificial Chromosomes (BACs). Genomics 33: 9-20.