Chlorfenvinphos, an organophosphate insecticide, affects liver mitochondria antioxidative enzymes, glutathione and hydrogen peroxide concentration

• Autorzy: Lukaszewicz-Hussain A , Moniuszko-Jakoniuk J.
• Języki publikacji: EN

Abstrakty

EN

Chlorfenvinphos is an organophosphate insecticide widely used alone or in combination in Poland. In the present study, the influence of chlorfenvinphos on the activity of mitochondrial antioxidative system as well as hydrogen peroxide concentration was examinated. The experiments were conducted on male Wistar rats of 180±20g body weight. The animals were divided into two groups: the control group, which received oil intragastrically by stomach tube and the experimental groups, which received oil solution of chlorfenvinphos in doses of 0.02 LD50, 0.1 LD50 or 0.5 LD50. After 1, 24, 48 hours the livers were quickly removed. Liver mitochondria were isolated as described elsewhere. Glutathione peroxidase, superoxide dismutase, isocitrate dehydrogenase activity as well as reduced glutathione and hydrogen peroxide concentrations were determined in liver mitochondria using BIOXYTECH Assay kits produced by OXIS International, Inc., Portland, USA. The results of this work indicate that chlorfenvinphos induces oxidative stress to rat liver mitochondria. In acute chlorfenvinphos intoxication, we demonstrated that the key role in the oxidative mitochondrial damage play MnSOD and GSH pool, as well as accumulation of hydrogen peroxide.

Słowa kluczowe

EN

chemical pollutant; chlorfenvinphos; xenobiotic; mitochondrion; hydrogen peroxide; drug; glutathione; liver; organophosphate insecticide; antioxidative enzyme

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2004
• Tom: 13
• Numer: 4
• Opis fizyczny: p.397-401,ref.

Twórcy

autor

Lukaszewicz-Hussain A

• Medical Academy in Bialystok, 2C Mickiewicza st., 15-869 Bialystok, Poland

autor

Moniuszko-Jakoniuk J.

Bibliografia

• 1. STURGILL M.C., LAMBERT G.H.: Xenobiotic-induced hepatotoxicity: mechanism of liver injury and methods of monitoring hepatic function. Clin. Chim. 43, 1512, 1997
• 2. JAESCHKE H., GORES J., CEDERBAUM A.I., HINSON J.A., PESSAYRE D., LEMASTER J.J.: Mechanism of hepatotoxicity. Toxicol. Sci. 65, 166, 2002
• 3. JO S.H., SON M.K., KOH H.J., LEE S.M., SONG I.H., KIM Y.O., LEE Y.S., JEONG K.S., KIM W.B., PARK J.W., SONG B.J., HUH T.L.: Control of mitochondrial redox balance and cellular defense against oxidative damage by mitochondrial NADP+-dependent isocitrate dehydrogenase (IDPm). J. Biol. Chem. 276, 16168, 2001
• 4. KOWALTOWSKI A.J, VERCESI A.E.: Mitochondrial damage induced by conditions of oxidative stress. Free Radic. Biol. Med. 26, 463, 1999
• 5. ADACHII M., ISHII H.: Role of mitochondria in alkoholic liver injury. Free Rad. Biol. Med. 32, 487, 2002
• 6. ARAI M., IMAI H., KOUMURA T., YOSHIDA M., EMOTO K., UMEDA M., CHIBA N., NAKAGAWA Y.: Mitochondrial phospholipid hydroperoxide glutathione per- oxidase plays a major role in preventing oxidative injury to cells. J. Biol. Chem. 274, 4924, 1999
• 7. LUKASZEWICZ-HUSSAIN A., MONIUSZKO-JAKO- NIUK J.: Activities of superoxide dismutase and catalase in erythrocytes and concentration of malondialdehyde in serum of rats intoxicated with chlorfenvinfos in low doses. Pol. J. Environ. Stud. 8, Suppl. II, 234, 1999
• 8. BEATTIE D.S.: Enzyme localization in the inner and outher membranes of rat liver mitochondria. Biochem. Biophys. Res. Commun. 31, 902, 1968
• 9. LOWRY O.H., ROSENBROUGH M.J., FARR A.L., RAN­DALL R.: Protein measurement with the Folin phenol re­agent. J. Biol. Chem. 193, 263, 1951
• 10. WHEELER M.D., NAKAGAMI M., BRADFORD B.U., UESUGI T., MASON R.P., CONNOR H.D., DIKALOWA A., KADIISKA M., THURMAN R.G.: Overexpression of mangenase superoxide dismutase prevents alkoholinduced liver injury in the rat. J. Biol. Chem. 276, 36664, 2001
• 11. ZWACKA R. M., ZHOU Y., ZHANG Y., DARBY C. J., DUDUS L., HALLDORSON J., OBERLEY L., ENGEL­HARDT J. F.: Redox gene therapy for ischemia/repefusion injury of the liver reduces API and NF-kappa B activation. Nat. Med. 4, 698, 1998
• 12. ZHANG Z., APSE K., PANG J., STANTON R.C.: High glu­cose inhibits glucose-6-phosphate dehydrogenase via cAMP in aortic endothelial cells. J. Biol. Chem. 275, 40042, 2000
• 13. LU S.C.: Regulation of hepatic glutathione synthesis: cur­rent concepts and controversies. FASEB J. 13, 1169, 1999
• 14. MARTIN M., MACIAS M., ESCAMES G., LEON J., ACUNA-CASTROVIEJO D.: Melatonin but not vitamins C and E maintains glutathione homeostasis in t-butyl hydro- peroxide-induced mitochondrial oxidative stress. FASEB J., published on line 24. 06. 2000
• 15. MERAD-SAIDOUNE M., BOITIER E., MARSAC A.N., MARTNOU J.C., SOLA B., SINET P.M., CEBALLOS-PI- COT I.: Overproduction of Cu/Zn-superoxide dismutase or Bcl-2 prevents the brain mitochondrial respiratory dysfunction induced glutathione depletion. Exp. Neurol. 158, 428, 1999
• 16. TIAN W.N., BRAUSTEIN L.G., APSE K., PANG J., ROSE M., TIAN X., STANTON R.C.: Importance of glucose- 6-phosphate dehydrogenase activity in cell death. Am. J. Physiol. 276, C1121b, 1999
• 17. SALVEMINI F., FRANZE A., IERVOLINO A., FILOSA S., SALZANO S., URSINI M.V.: Enhanced glutathione levels and oxidoresistance mediated by increased glucose-6-phos- phate dehydrogenase expression J. Biol. Chem. 1999, 274, 2750, 1999