Zastosowanie analizy makrorestrykcyjnej do roznicowania wybranych szczepow z grupy Bacillus cereus

• Autorzy: Niemcewicz M , Bartoszcze M.

Warianty tytułu

EN

Applying macro-restriction analysis in differentiating selected strains of the Bacillus cereus group

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to apply macro-restriction analysis in order to differentiate selected strains of the Bacillus cereus group. Three different restriction enzymes (SfiI, NotI and SmaI) were used during the research process and it was confirmed that SmaI is the best restriction enzyme applied in macrorestriction analysis of the Bacillus cereus group. The study used 89 Bacillus cereus group strains, and an identical restriction pattern generated by restriction enzymes was confirmed among 23 of the studied Bacillus anthracis strains. Two groups of strains (four and two strains) among 19 Bacillus sp. Ba 813 and three groups (three, two and two strains) among 44 strains of Bacillus thuringiensis showed identical restriction profiles. The similarity of studied strains was ascertained on the basis of the Dice coefficient, and confirmed that strains showing the same restriction profiles were identical (100% similarity). The similarity between the remaining strains of the Bacillus cereus group and the Bacillus anthracis strains oscillated between 20% to 78.6%, while the similarity of these strains to each other oscillated from 15.4 % to 100%. Dendrograms were also constructed using the UPGMA method.

Słowa kluczowe

PL

Bacillus cereus; szczepy bakteryjne; analiza makrorestrykcyjna; bakterie; roznicowanie

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 09
• Opis fizyczny: s.1065-1070,rys.,bibliogr.

Twórcy

autor

Niemcewicz M

• Wojskowy Instytut Higieny i Epidemiologii, ul.Lubelska 2, 24-100 Pulawy

autor

Bartoszcze M.

Bibliografia

• 1.Agvald-Öhman C., Lund B., Edlund C.: Multiresistant coagulase-negative staphylococci disseminate frequently between intubated patientsin a multidisciplinary intensive care unit. Critical Care 2004, 8, R42-R47.
• 2.Ahmed R., Sankar-Mistry P., Jackson S., Ackermann H.-W., Kasatiya S. S.: Bacillus cereus phage typing as an epidemiological tool in outbreaks of food poisoning. J. Clin. Microbiol. 1995, 33, 636-640.
• 3.Antonishyn N. A., McDonald R. R., Chan E. L., Horsman G., Woodmansee C. E., Falk P. S., Mayhall C. G.: Evaluation of fluorescence-based amplified fragment length polymorphism analysis for molecular typing in hospital epidemiology: comparison with pulsed-field gel electrophoresis for typing strains of vancomycin-resistant Enterococcus faecium. J. Clin. Microbiol. 2000, 38, 4058-4065.
• 4.Aronson A. I., Beckman W., Dunn P.: Bacillus thuringiensis and related insect pathogens. Microbiological Rev. 1986, 50, 1-24.
• 5.Basim E. H., Basim H.: Pulsed-field gel electrophoresis (PFGE) technique and its use in molecular biology. Turk. J. Biol. 2001, 25, 405-418.
• 6.Bergey's Manual of Determinative Bacteriology. Wyd. IX, Williams and Wilkins Company. Baltimore 1994.
• 7.Blanc D. S., Struelens M. J., Deplano A., De Ryck R., Hauser P. M., Petignat C., Francioli P.: Epidemiological validation of pulsed-field gel electrophoresis patterns for methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 2001, 39, 3442-3445.
• 8.Brumlik M. J., Szymajda U., Zakowska D., Liang X., Redkar R. J., Patra G., DelVecchio V. G.: Use of long-range repetitive element polymorphism-PCR to differentiate Bacillus anthracis strains. Appl. Environ. Microbiol. 2001, 67, 3021-3028.
• 9.Carlson C. R., Kolstø A.-B.: A small (2.4 Mb) Bacillus cereus chromosome corresponds to a conserved region of a larger (5.3 Mb) Bacillus cereus chromosome. Molec. Microbiol. 1994, 13, 161-169.
• 10.Cherif A., Borin S., Rizzi A., Ouzari H., Boudabous A., Daffonchio D.: Bacillus anthracis diverges from related clades of the Bacillus cereus group in 16S-23S ribosomal DNA intergenic transcribed spacers containing tRNA genes. Appl. Environ. Microbiol. 2003, 69, 33-40.
• 11.Daffonchio D., Borin S., Frova G., Gallo R., Mori E., Fani R., Sorlini C.: A randomly amplified polymorphic DNA marker specific for the Bacillus cereus group is diagnostic for Bacillus anthracis. Appl. Environ. Microbiol. 1999, 65, 1298-1303.
• 12.Ezzell Jr. J. W., Abshire T. G., Little S. F., Lidgerding B. C., Brown C.: Identification of Bacillus anthracis by using monoclonal antibody to cell wall galactose-N-acetylglucosamine polysaccharide. J. Clin. Microbiol. 1990, 28, 223-231.
• 13.Gouby A., Carles-Nurit M.-J., Bouziges N., Bourg G., Mesnard R., Bouvet P. J. M.: Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of acinetobacter baumannii. J. Clin. Microbiol. 1992, 30, 1588-1591.
• 14.Hansen S. M., Uldbjerg N., Kilian M., Sørensen U. B. S.: Dynamics of Streptococcus agalactiae colonization in woman during and after pregnancy and in their infants. J. Clin. Microbiol. 2004, 42, 83-89.
• 15.Harrell L. J., Andersen G. L., Wilson K. H.: Genetic variability of Bacillus anthracis and related species. J. Clin. Microbiol. 1995, 33, 1847-1850.
• 16.Hathout Y., Demirev P. A., Ho Y.-P., Bundy J. L., Ryzhov V., Sapp L., Stutler J., Jackman J., Fenselau C.: Identification of Bacillus spores by matrix-assisted laser desorption ionization-mass spectrometry. Appl. Environ. Microbiol. 1999, 65, 4313-4319.
• 17.Helgason E., Okstad O. A., Caugant D. A., Johansen H. A., Fouet A., Mock M., Hegna I., Kolstø A.-B.: Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis - one species on the basis of genetic evidence. Appl. Environ. Microbiol. 2000, 66, 2627-2630.
• 18.Hill K. K., Ticknor L. O., Okinaka R. T., Asay M., Blair H., Bliss K. A., Laker M., Pardington P. E., Richardson A. P., Tonks M., Beecher D. J., Kemp J. D., Kolstø A.-B., Lee Wong A. C., Keim P., Jackson P. J.: Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis isolates. Appl. Environ. Microbiol. 2004, 70, 1068-1080.
• 19.Hoa N. T., Baccigalupi L., Huxham A., Smertenko A., Van P. H., Ammendola S., Ricca E., Cutting S. M.: Characterization of Bacillus species used for oral bacteriotherapy and bacterioprophylaxis of gastrointestinal disorders. Appl. Environ. Microbiol. 2000, 66, 5241-5247.
• 20.Huang E. T. S., Chu Y.-W., Ng T.-K., Cheng A. F. B.: Study of the relatedness of isolates of Shigella flexneri and in 1994 and 1995 from Hong Kong. J. Clin. Microbiol. 1998, 36, 2404-2407.
• 21.Lai E., Birren B. W., Clark S. M., Simon M. I., Hood L: Pulsed field gel electrophoresis. BioTechniques 1989, 7, 34-42.
• 22.Liu P. Y.-F., Ke S.-C., Chen S.-L.: Use of pulsed-field gel electrophoresis to investigate a pseudo-outbreak of Bacillus cereus in a pediatric unit. J. Clin. Microbiol. 1997, 35, 1533-1535.
• 23.Mőllby R., Kühn I., Katouli M.: Computerised biochemical fingerprinting - a new tool for typing of bacteria. Rev. Med. Microbiol. 1993, 4, 231-241.
• 24.Niemcewicz M., Patra G., Redkar R., DelVecchio V. G.: Utilization of the NotI and SmaI Bacillus anthracis pulsotype patterns as chromosomal signature. Mat. Nauk. Konf.: Protection against Biological Threats, Warszawa 2001, s. 73.
• 25.Patra G. P., Niemcewicz M., Zakowska D., DelVecchio V. G.: Use of pulsed-field gel electrophoresis to investigate the whole genome organization in the Bacillus cereus group of Bacteria. 4th Internat. Conf. Anthrax, Annapolis, Maryland, USA, 2001, Abstract Book 22.
• 26.Patra G., Vaissaire J., Weber-Levy M., Le Doujet C., Mock M.: Molecular characterization of Bacillus strains involved in outbreaks of anthrax in France in 1997. J. Clin. Microbiol. 1998, 36, 3412-3414.
• 27.Radnedge L., Agron P. G., Hill K. K., Jackson P. J., Ticknor L. O., Keim P., Andersen G. L.: Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Appl. Environ. Microbiol. 2003, 69, 2755-2764.
• 28.Ramisse V., Patra G., Garrigue H., Guesdon J.-L., Mock M.: Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA. FEMS Microbiology Letters 1996, 145, 9-16.
• 29.Ramisse V., Patra G., Vaissaire J., Mock M.: The Ba813 chromosomal DNA sequence effectively traces the whole Bacillus anthracis community. J. Appl. Microbiol. 1999, 87, 224-228.
• 30.Rennie R. P., Strong D., Taylor D. E., Salama S. M., Davidson C., Tabor H.: Campylobacter fetus diarrhea in a Hutterite colony: epidemiological observations and typing of the causative organism. J. Clin. Microbiol. 1994, 32, 721-724.
• 31.Ryzhov V., Hathout Y., Fenselau C.: Rapid characterization of spores of Bacillus cereus group bacteria by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. Appl. Environ. Microbiol. 2000, 66, 3828-3834.
• 32.Shangkuan Y.-H., Yang J.-F., Lin H.-C., Shaio M.-F.: Comparison of PCR-RFLP, ribotyping, and ERIC-PCR for typing Bacillus anthracis and Bacillus cereus strains. J. Appl. Microbiol. 2000, 89, 452-462.
• 33.Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B.: Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 1995, 33, 2233-2239.
• 34.Zwet W. C. Van Der, Parlevliet G. A., Savelkoul P. H., Stoof J., Kaiser A. M., Van Fourth A. M., Vandenbroucke-Grauls C. M.: Outbreak of Bacillus cereus infections in a neonatal intensive care unit traced to balloons used in manual ventilation. J. Clin. Microbiol. 2000, 38, 4131-4136.