Induction of multi-nucleate microspores in anther culture of salix viminalis L.

• Autorzy: Wojciechowicz M K , Kikowska M.A.
• Języki publikacji: EN

Abstrakty

EN

The aim of the work was to determine how the induction of androgenesis in selected genotypes of Salix viminalis is affected by thermal factors and medium composition. Anthers isolated from male clones of S. viminalis were pre-cultured at 4, 27 and 32°C for two to eight days in liquid Kyo medium with and without the addition of mannitol and on solid MS or WPM medium. The solid media were supplemented with different concentration of disaccharidesand various combinations of growth regulators including kinetin, 2iP, IAA and IBA. Multi-nucleate microspores indicative of androgenesis were observed in anthers pre-cultured for seven days at 4°C in liquid Kyo medium containing mannitol. The rate of androgenesis was higher when the anthers were transferred to solid modified MS medium containing high concentrations of sucrose and kinetin. In studied genotypes of basket willow early uninucleate microspores underwent sporophytic divisions. Ultrastructural observations showed differences in cellular arrangement of pre-stressed microspores. In Salix viminalis, microspores without starch grains and decreased number of lipid bodies were potentially androgenic cells.

Słowa kluczowe

EN

Salix viminalis; genotype; androgenesis; thermal factor; medium composition; stress response; thermal shock; anther culture; multinucleate microspore; male clone; mannitol; Kyo medium; microspore; ultrastructural analysis; initial microspore

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2009
• Tom: 61
• Opis fizyczny: p.55-64,fig.,ref.

Twórcy

autor

Wojciechowicz M K

• Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland

autor

Kikowska M.A.

Bibliografia

• Arrillaga I., Lerma V., Pérez-Bermúdez P., Seruga J. 1995. Callus andsomatic embryogenesis from culturedanthers of service tree (Sorbus domestica L.). HortScience 30: 1078–1079.
• Baldursson S., Krogstrup P., Nørgaard J.V., Andersen S.B. 1993. Anther culture of tree species of Populus and regeneration of haploidplants of Populus trichocarpa. Canadian Journal of Forest Research 23: 1821–1825.
• Baldursson S., Ahuja M.R. 1996. Haploids in forest trees. In: In vitro haploidprod uction in higher plants Vol. 3. Mohan Jain S., Sopory S.K., Veilleux R.E. (eds). Kluwer Academic Publishers, pp. 297–336.
• Bueno M.A., Gómez A., Boscaiu M., Manzanera J.A., Vicente O. 1997. Stress-induced formation of haploidplants through anther culture in cork oak (Quercus suber). Physiolgia Plantarum 99: 335–341.
• Bueno M.A., Pintos B., Höfer M., Martin A. 2005. Pro-embryo induction from Olea europea L. isolatedmicrospore culture. Acta Physiologiae Plantarum 27: 695–701.
• Chen Z., Qian C., Qin M., Xu X., Xiao Y. 1982. Recent advances in anther culture of Hevea brasiliensis (Muell. Arg.). Theoretical andAppliedGenetics 62: 103–108.
• Chen Z. 1985. A study on induction of plants from Citrus pollen. Fruit Varieties Journal 39: 44–50.
• Chu C.C. 1978. The N6 medium and its applications to anthers cultures of cereal crops. In: Proceedings of Symposium on Tissue Culture, Science Press Beijing, pp. 43–50.
• Deutsch F., Kumlehn J., Ziegenhagen B., Fladung M. 2004. Stable haploidpoplar callus lines from immature pollen culture. Physiologia Plantarum 120: 613–622.
• Forster B.P., Heberle-Bors E., Kasha K.J., Touraev A. 2007. The resurgence of haploids in higher plants. Trends in Plant Science 12: 368–375.
• Gautam V.K., Nanda K., Gupta S.C. 1993. Development of shoots androots in anther-derived callus of Azidirachta indica A. Juss-a medicinal tree. Plant Cell, Tissue and Organ Culture 34: 13–18.
• Germana M.A., Chiancone B. 2003. Improvement of Citrus clementina Hort. ex Tan. microspore – derived embryo idinduction andregeneration. Plant Cell Reports 22:181–187.
• Germana M.A. 2006. Double haploids production in fruit crops. Plant Cell, Tissue andOrgan Culture 86: 131–146.
• Gharyal P.K., RashidA., Maheshwari S.C. 1983. Production of haploid plants in the anther cultures of Albizzia lebbeck L. Plant Cell Reports 2: 308–309.
• Heberle-Bors E. 1998. Experimental control of pollen development. In: Androgenesis and Haploid Plants. Chupeau Y, Caboche M, Henry Y (eds). Springer, Berlin, pp. 38–53.
• Hosp J., Maraschin S.F., Touraev A., Boutilier K. 2007. Functional genomic of microspore embryogenesis. Euphytica 158: 275–285.
• Höfer M., Touraev A., Heberle-Bors E. 1999. Induction of embryogenesis from isolatedapple microspores. Plant Cell Reports 18: 1012–1017.
• Höfer M. 2004. In vitro androgenesis in apple – improvement of the induction phase. Plant Cell Reports 22: 365–370.
• Indrianto A., Heberle-Bors E., Touraev A. 1999. Assessment of various stresses andcarbohyd rates for their effect on the induction of embryogenesis in isolatedwheat microspores. Plant Science 143:71–79.
• Kadota M., Niimi Y. 2004. Production of triploid plants of Japanese pear (Pyrus pyrifolia Nakai) by anthers culture. Euphytica 138: 141–147.
• Kiss J., Kondrak M., Törjek O., Gyulai G., Mázik-Tokei K., Heszky L.E. 2001. Morphological and RAPD analysis of poplar trees of anther culture origin. Euphytica 118: 213–221
• Kyo M., Harada H. 1986. Control of the developmental pathway of tabacco pollen in vitro. Planta 168: 427–432.
• LloydG., McCown B. 1981. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia by use shoot tip culture. Combined Proceedings, International Plant Propagator’s Society 30:421–427.
• Miller C.O. 1965. Evidence for the natural occurrence of zeatin and derivates: compounds from maize which promote cell division. Proceedings of The National Academy of Science of The United States of America 54: 1052–1058.
• Maraschin S.F., de Priester W., Spaink H.P., Wang M. 2005. Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective. Journal of Experimental Botany 56: 1711–1726.
• Murashige T., Skoog F. 1962. A revised medium for rapidgrowth andbio-assays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497.
• Nitsch J.P. 1972. Haploidplants from pollen. Zeitschrift für Pflanzenzüchtung 67: 3–18.
• Ochatt S.J., Zhang Y.X. 1996. In vitro haploidization of fruit trees. In: In Vitro Haploid Production in Higher Plants Vol. 2. Jain S. M., Sopory S.K., Veilleux R.E. (eds). Kluwer Academic Publishers, pp. 193–210.
• Palmer C.E., Keller W.A. 1997. Pollen embryos. In: Pollen Biotechnology for Crop Production and Improvement. Shivanna K.R., Sawhney V.K. (eds.). Cambridge University Press, pp. 292–422.
• Perera P.I.P., Hocher V., Verdeil J.L., Bandupriya H.D.D., Yakandawala D.M.D., Weerakoon L.K. 2008. Androgenic potential in coconut (Cocos nucifera L.). Plant Cell, Tissue andOrgan Culture 92: 293–302.
• PulfordI.D., Watson C.P. 2003. Phytoremediation of heavy metal-contaminatedland by trees a review. Environment International 29: 529–540.
• Radojevic L. 1991. Horse chestnut (Aesculus ssp.). In: Biotechnology in Agriculture andForestry Vol. 16 Trees III. Bajaj Y.P.S. (ed.). Springer-Verlag, Berlin pp. 111–141.
• Ramirez C., Chiancone B., Testillano P.S., Garcia-Fojeda B., Germana M.A., Risueno M.C. 2003. First embryogenic stages of Citrus microspore-derived embryos. Acta Biologica Cracoviensia Series Botanica 45: 53–58.
• Rodrigues L.R., Oliveira J.M.S., Mariath J.E.A. Iranco L.B., Bodanese-Zenettini M.H. 2005. Anther culture andcoldtreatment of floral buds increased symmetrical andextra nuclei frequencies in soybean pollen grains. Plant Cell, Tissue andOrgan Culture 81: 101–104.
• Redenbaugh M.K., Westfall R.D., Karnosky D.F. 1981. Dihaploidcallus production from Ulmus americana anthers. Botanical Gazette 142: 19–26.
• Smykal P. 2000. Pollen embryogenesis – the stress mediated switch from gametophytic to sporophytic development. Current status and future prospects. Biologia Plantarum 43: 481–489.
• Spurr A.R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 23: 31–43.
• Srivastava P., Chaturvedi R. 2008. In vitro androgenesis in tree species: An update and prospects for further research. Biotechnology Advances 26: 482–491.
• Stoehr M.U., Zsuffa L. 1990. Induction of haploids in Populus maximowiczii via embryogenic callus. Plant Cell, Tissue and Organ Culture 23: 49–58.
• Touraev A., Vicente O., Heberle-Bors E. 1997. Initiation of microspore embryogenesis by stress. Trends in Plant Science 2: 297–302.
• Wang M., van Bergen S., van Dujin B. 2000. Insights into a key development switch and its importance for efficient plant breeding. Plant Physiology 124: 523–530.
• Zenkteler M. 1984. Uzyskiwanie roślin haploidalnych metodą hodowli in vitro. In: Hodowla komórek i tkanek roślinnych. Zenkteler M. (ed.). PWN Warszawa, pp. 261–311.