Nitric oxide and platelet energy metabolism

• Autorzy: Tomasiak M. , Stelmach H. , Rusak T. , Wysocka J.
• Języki publikacji: EN

Abstrakty

EN

This study was undertaken to determine whether nitric oxide (NO) can affect platelet responses through the inhibition of energy production. It was found that NO donors: S-nitroso-.N-acetylpenicyllamine, SNAP, (5-50 uM) and sodium nitro- prusside, SNP, (5-100 uM) inhibited collagen- and ADP-induced aggregation of por­cine platelets. The corresponding IC50 values for SNAP and SNP varied from 5 to 30 uM and from 9 to 75 uM, respectively. Collagen- and thrombin-induced platelet secre­tion was inhibited by SNAP (IC50 = 50 uM) and by SNP (IC50 = 100 uM). SNAP (20-100 uM), SNP (10-200 uM) and collagen (20 ug/ml) stimulated glycolysis in in­tact platelets. The degree of glycolysis stimulation exerted by NO donors was similar to that produced by respiratory chain inhibitors (cyanide and antimycin A) or uncouplers (2,4-dinitrophenol). Neither the NO donors nor the respiratory chain blockers affected glycolysis in platelet homogenate. SNAP (20-100 uM) and SNP (50-200 uM) inhibited oxygen consumption by platelets. The effect of SNP and SNAP on glycolysis and respiration was not reduced by 1H-[1,2,4]oxadiazo- lo-[4,3-a]quinoxalin-1-one, a selective inhibitor of NO-stimulated guanylate cyclase. SNAP (5-100 ,aM) and SNP (10-300 uM) inhibited the activity of platelet cytochrome oxidase and had no effect on NADH:ubiquinone oxidoreductase and succinate dehydrogenase. Blocking of the mitochondrial energy production by antimycin A slightly affected collagen-evoked aggregation and strongly inhibited platelet secre­tion. The results indicate that: 1) in porcine platelets NO is able to diminish mito- chondrial energy production through the inhibition of cytochrome oxidase, 2) the in­hibitory effect of NO on platelet secretion (but not aggregation) can be attributed to the reduction of mitochondrial energy production.

Słowa kluczowe

EN

porcine platelet; metabolism; glycolysis; energy production; nitric oxide; mitochondrial energy production

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 3
• Opis fizyczny: p.789-803,fig.,ref.

Twórcy

autor

Tomasiak M.

• Medical University of Bialystok, J.Kilinskiego 1, 15-089 Bialystok, Poland

autor

Stelmach H.

autor

Rusak T.

autor

Wysocka J.

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