Purification of the Ca2 -ATPase from rat cortical, cerebellar and hippocampal synaptosomal membranes by two - step chromatography

• Autorzy: Zylinska L. , Lachowicz L. , Gromadzinska E.
• Języki publikacji: EN

Abstrakty

EN

Ca2+-ATPase, the enzyme responsible for maintenance of low resting Ca2+ level in the nerve cell, has been purified from rat cortical, cerebellar and hippocampal synaptosomal membranes by affinity chromatography on Calmodulin-Agarose which followed Reactive Red 120-Agarose column. The enzymes from these three regions ran as broad, monomeric bands on SDS-PAGE with a molecular weight at range 130-138 kDa, and were identified as a Ca2+- ATPase using monoclonal antibody 5F10. Analysis of the kinetic parameters revealed that hippocampal Ca2+-ATPase exhibited 2 times higher affinity for ATP, than cortical and cerebellar enzymes. The affinity for Ca2+ increased in order: cerebellum, cortex, and hippocampus. The differences in kinetic characteristics of purified enzymes, suggest that in adult rat brain the Ca2+-ATPase could be represented at a protein level by the region-dependent combination of several isoforms.

Słowa kluczowe

EN

agarose; brain; enzyme; isoenzyme; calmodulin; purification; neuron; antibody; plasma membrane; chromatography; membrane; cerebellum; calcium ion; hippocampus; cortex

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1998
• Tom: 03
• Numer: 1
• Opis fizyczny: p.37-48,fig.

Twórcy

autor

Zylinska L.

• Medical University of Lodz, 6 Lindley Street, 90-131 Lodz, Poland

autor

Lachowicz L.

autor

Gromadzinska E.

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