Cloning, expression, and crystallization of Cpn60 proteins from Thermococcus litoralis

• Autorzy: Osipiuk J. , Sriram M. , Mai X. , Adams M.W.W. , Joachimiak A.
• Języki publikacji: EN

Abstrakty

EN

Two genes of the extreme thermophilic archaeon Thermococcus litoralis homologous to those that code for Cpn60 chaperonins were cloned and expressed in Escherichia coli. Each of the Cpn60 subunits as well as the entire Cpn60 complex crystallize in a variety of morphological forms. The best crystals diffract to 3.6 resolution at room temperature and belong to the space group I422 with unit cell parameters a = b = 193.5 A, c = 204.2 A.

Słowa kluczowe

EN

gene; Cpn60 chaperonin; crystallization; cloning; Cpn60 protein; Thermococcus litoralis; Escherichia coli; expression

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2000
• Tom: 47
• Numer: 1
• Opis fizyczny: p.209-214,fig.

Twórcy

autor

Osipiuk J.

• Argonne National Laboratory, 9700 S.Cass Ave., Argonne, IL 60439, USA

autor

Sriram M.

autor

Mai X.

autor

Adams M.W.W.

autor

Joachimiak A.

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