Photodynamic effects of two water soluble porphyrins evaluated on human malignant melanoma cells in vitro

• Autorzy: Szurko A. , Kramer-Marek G. , Widel M. , Ratuszna A. , Habdas J. , Kus P.
• Języki publikacji: EN

Abstrakty

EN

Two water soluble porphyrins: meso-tetra-4-N-methylpyridyl-porphyrin iodide (P1) and 5,10-di-(4-acetamidophenyl)-15,20-di-(4-N-methylpyridyl) porphyrin (P2) were synthesised and evaluated in respect to their photochemical and photophysical properties as well as biological activity. Cytotoxic and phototoxic effects were evaluated in human malignant melanoma Me45 line using clonogenic assay, cytological study of micronuclei, apoptosis and necrosis frequency and inhibition of growth of megacolonies. Both porphyrins were characterised by high UV and low visible light absorptions. Dark toxicity measured on the basis of the clonogenic assay and inhibition of megacolony growth area indicated that P1 was non-toxic at concentrations up to 50 ug/ml (42.14 uM) and P2 at concentrations up to 20 ug/ml (16.86 uM). The photodynamic effect induced by red light above 630 nm indicated that both porphyrins were able to inhibit growth of melanoma megacolonies at non-toxic concentrations. Cytologic examination showed that the predominant mode of cell death was necrosis.

Słowa kluczowe

EN

water soluble porphyrin; man; cell; necrosis; photodynamic therapy; in vitro; apoptosis; tumour; synthetic porphyrin; photodynamic effect; malignant melanoma; porphyrin

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 4
• Opis fizyczny: p.1165-1174,fig.,ref.

Twórcy

autor

Szurko A.

• University of Silesia, Uniwersytecka 4, 40-007 Katowice, Poland

autor

Kramer-Marek G.

autor

Widel M.

autor

Ratuszna A.

autor

Habdas J.

autor

Kus P.

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