Selected immunological and haematological indices in breams [Abramis brama] inhabiting various aquatic ecosystems

• Autorzy: Stosik M , Deptula W. , Deptula-Tokarz B.
• Języki publikacji: EN

Abstrakty

EN

Physiological parameters in fish provide grounds for conclusions as to the physiological status of the organism. The status is directly related to inner and outer factors, biotic and abiotic influences which act on the organism. Present studies aimed at defining the set of immunological and haematological indices in breams, which form fish populations in Dabie Lake and Szczecin Bay, whose ecosystems differ in water cleanness. Immunological studies in breams involved determining selective defensive functions of neutrophilic granulocytes, expressed by the index of phagocytosis of a standard bacterial strain (Ipg), the percentage of phagocytes which ingested the bacteria (%gp), NBT index, the amount of formazan and the index of myeloperoxidase activity (WA MPO). Haematological studies included determination of absolute leukocyte number and the differential leukocyte pattern. Bacteriological and physicochemical studies were also performed on water samples of reservoir sites from which the fish originated. Results of examination of water samples from Dabie Lake and Szczecin Bay demonstrated a significant level of water pollution in the ecosystems. A definitely higher level of water pollution, particularly in respect to bacterial contamination, was observed in Dabie Lake. Comparison of immunological and haematological results demonstrated significant differences between the two studied bream populations, i.e. those originating from Dabie Lake and the other, originating from Szczecin Bay. Such differences were disclosed both in immunological and in haematological indices.

Słowa kluczowe

EN

fish; environment protection; physiological parameter; neutrophilic granulocyte; aquatic ecosystem; bream; Abramis brama; environment condition; leucocyte

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2002
• Tom: 11
• Numer: 3
• Opis fizyczny: p.273-277

Twórcy

autor

Stosik M

• University of Zielona Gora, Monte Cassino 21b, 65-561 Zielona Gora, Poland

autor

Deptula W.

autor

Deptula-Tokarz B.

Bibliografia

• 1. DEPTULA W. Phagocytic activity of neutrophilic granulocytes (PMN cells) in peripheral blood of cattle infected with IBR/IPV virus (bovine Herpesvims 1) (in Polish). Pol. Arch. Wet 31, 153, 1991.
• 2. OSTROWSKA A., GAWLINSKI S., SZTUBIALKA Z. Methods of water analysis and of evaluation of water properties. Catalogue, part II (in Polish), Wyd. Inst Ochrony Srodowiska, Warszawa., 1991.
• 3. PN-75/C-04615. Water and sewage. Microbiological testing (in Polish).
• 4. RAMAN U., POLAND R.L. A new micro-quantitative nitroblue tetrazolium test. Pediatr. Res. 9, 334, 1975.
• 5. Instruction of Ministry of Health and Social Welfare of 4h May, (in Polish), altering instruction on requirements which should be fulfilled by drinking water and water for house hold use. Supplement No. 2. 1990.
• 6. Instruction of Minister for Environmental Protection, Natural Resources and of Forestry of 5,h November, (in Polish) pertaining classification of waters and requirements as to sewage draining to water or to ground. 1991.
• 7. SIWICKI A., STUDNICKA M., RYKA B. Phagocytic activity of neutrophils in carp. Bamidgeh 37, 123, 1985.
• 8. STOSIK M. Reference physiological indices in breams (in Polish), worked out for the needs of Voivodship Veterinary Hospital in Zielona Gora (unpublished data), 1999.
• 9. STOSIK M. Anatomopathological alterations and values of selected parameters of non-specific immunity in carps originating from ponds with erythrodermatitis-infected fish (in Polish). Medycyna Wet. 46, 440, 1990.
• 10. STOSIK M. Activity of neutrophilic granulocytes of carps in the course of natural bacterial infection (in Polish). Immunol. Pol. 16, 131, 1991.
• 11. STOSIK M., DEPTULA W. Action of chemical substances on protective mechanisms in fish (in Polish). Medycyna Wet. 54: 583, 1998.
• 12. SYCHLOWY A., LUKAS A. Evaluation of microquantitative technique of NBT reduction by peripheral blood granulocytes (in Polish). Pol. Tyg. Lek. 33, 45, 1978.
• 13. ZAWISTOWSKI S. Histological technique, principles of histology and histology (in Polish). PZWL. Warszawa. p. 215, 1975