Effect of heavy metal pollution on genetic variation and cytological disturbances in the Pinus sylvestris L. population

• Autorzy: Prus-Glowacki W , Chudzinska E , Wojnicka-Poltorak A , Kozacki L , Fagiewicz K
• Języki publikacji: EN

Abstrakty

EN

This isoenzymatic and cytogenetic study has shown significant differences in genetic composition between two groups of Pinus sylvestris trees: tolerant and sensitive to heavy metal pollution. Total and mean numbers of alleles and genotypes per locus were higher in the pollution-sensitive group of trees, but heterozygosity (H₀) was lower in this group. Fixation index (F) indicates that trees tolerant for pollution were in the Hardy-Weinberg equilibrium, while the sensitive group had a significant excess of homozygosity. Cytological analyses demonstrated numerous aberrations of chromosomes in meristematic root tissue of seedlings developed from seeds collected from trees in the polluted area. The aberrations included chromosome bridges and stickiness, laggards, retarded and forward chromosomes, and their fragments. The mitotic index was markedly lower in this group of seedlings, as compared to the control. Both isoenzymatic and cytological analyses showed a significant influence of heavy metal ions on the genetic structure of the Pinus sylvestris population.

Słowa kluczowe

EN

genetics; Pinus sylvestris; isoenzyme; metal pollution; tree; cytological disturbance; chromosome aberration; population; heavy metal; genetic variation

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2006
• Tom: 47
• Numer: 2
• Opis fizyczny: p.99-108,fig.,ref.

Twórcy

autor

Prus-Glowacki W

• Department of Genetics, Institute of Experimental Biology, Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland

autor

Chudzinska E

• Department of Genetics, Institute of Experimental Biology, Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland

autor

Wojnicka-Poltorak A

• Department of Genetics, Institute of Experimental Biology, Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland

autor

Kozacki L

• Institute of Physical Geography and Environmental Planning, Adam Mickiewicz University, Poznan, Poland

autor

Fagiewicz K

• Institute of Physical Geography and Environmental Planning, Adam Mickiewicz University, Poznan, Poland

Bibliografia

• Bergmann F, Hozius B, 1996. Effect of heavy metal polluted soils on genetic structure of Norway spruce seedlings populations. Water, Air and Soil Pollution 89: 363-373.
• Bergmann F, Scholz F, 1987. Effects of selection pressure by SO₂ pollution on genetic structure of Norway spruce (Picea abies). Lect. Notes in Biomath 60: 267-275.
• Cairney JWG, Meharg A, 1999. Influences of anthropogenic pollution on mycorrhizal fungal communities. Environ Pollut 106: 169-182.
• Chałupka W, 1998. Pollen formed under pollution affects some quantitative characters of Scots pine (Pinus sylvestris) seeds. Forest Genetics 5:133-136.
• Chudzińska E, 2002. Chromosomal analyses of ozone-exposed seedlings of Picea abies (L.) Karst. Materials from Symposium of Population and Evolutionary Genetics of Forest Trees, Stara Lesna, Slovakia, August 25-29.
• Das P, Samantaray S, Rout GR, 1998. Studies on cadmium toxicity in plants: a review. Environ Pollut 98: 29-36.
• De Kok LJ, Stuiver CEE, Stulen I, 1998. Responses of plant metabolism to air pollution and global change. In: De Kok, Stulen I, eds. Backhuys Publishers, Leiden, The Netherlands: 51-63.
• Dixon RK, Buschena CA, 1988. Response of ectomycorrhizal Pinus banksiana and Picea glauca to heavy metals in soil. Plant Soil 105: 265-271.
• Duan HX, Wang L, 1995. Cytogenetical toxical effects of heavy metals on Vicia faba and inquires into the Vicia micronucleus. Acta Bot Sin 37: 14-24.
• El-Ghamery AA, El-Kholy MA, Abou El-Yousser MA, 2003. Evaluation of cytological effects of Zn⁺ in relation to germination and root growth of Nigella sativa L. and Triticum aestivum L. Mutat Res 537: 29-41.
• Geburek T, Scholz F, Knabe W, Vornweg A, 1987. Genetic studies by isozyme gene loci on tolerance and sensitivity in an air polluted Pinus sylvestris field trial. Silv Genet 36: 49-53.
• Goldbold DL, Jentschke G, Winter S, Marschner P, 1998. Ectomycorhizas and amelioration of metal stress in forest trees. Chemosphere 36: 757-762.
• Grant WF, Owens ET, 2001. Chromosome aberration assays in Pisum for the study of environmental mutagens. Review. Mutat Res 488: 93-118.
• Hertel H, Paul M, 2001. Field testing of model of Norway spruce (Picea abies L. Karst.) with different genetic structures reforestation in air polluted regions. In: Müller-Starck G, Schubert R, eds. Response of forest systems to changing environmental conditions. Kluwer Academic Publishers, Dordrecht, Boston, London 70: 339-352.
• Ishido M, Kunimoto M, 2000. Regulation of cell fate by Cadmium and Zinc. J Health Sci 47: 9-13.
• Kabata-Pendias A, Pendias H, 1999. Biogeochemia pierwiastków śladowych [Biogeochemistry of trace elements], PWN, Warszawa.
• Kabata-Pendias A, Pendias H, 2001. Trace elements in soil and plants, CRC Press Boca-Raton, USA.
• Kirkland D, 1998. Chromosome aberration testing in genetic toxicology - past, present and future. Mutat Res 404: 173-185.
• Korshikov II, Velikoridko TI, Butilskaya LA, 2002. Genetic structure and variation in Pinus sylvestris L. populations degrading due to pollution-induced injury. Silv Genet 51: 45-49.
• Kovalchuk O, Kovalchuk I, Arkhipov A, Telyuk P, Hohn В, Kovalchuk L, 1998. The Allium сера chromosome aberration test reliably measures genotoxicity of soils of inhabited areas in the Ukraine contaminated by Chernobyl accident. Mutat Res 415: 47-57.
• Kristen U, 1997. Use of higher plants as screens for toxicity assessment. Toxicology in vitro 11: 181-191.
• Liu DH, Liu WS, Jiang W, Wang L, Zhai L, 1995. Evaluation of metal ion toxicity on root tip cells by the Allium test. Israel J Plant Sci 43: 125-133.
• Mejnartowicz L, 1983. Changes in genetic structure of Scots pine (Pinus sylvestris L.) population affected by industrial emission of fluoride and sulfur dioxide. Genet Polon 24: 41-50.
• Mejnartowicz L, 2001. Influence of nursery environment and pollution on alders. In: Müller-Starck G, Schubert R, eds. Response of forest systems to changing environmental conditions. Kluwer Academic Publishers, Dordrecht, Boston, London 70: 63-73.
• Müller M, Grill D, 1996. Chromosomal aberrations in ozone-impacted spruce as a test of cytological damage in forest trees. Forest Genetics 3:161-166.
• Müller M, Tausz M, Guttenberger H, Grill D, 1998. Early detection of environmental influences by recording chromosomal defects in root tip meristems of spruce trees. Environ. Sci Pollut Res: 101-104.
• Müller-Starck G, 1987. Genetic differences between tolerant and sensitive beeches (Fagus sylvatica L.) in an environmentally stressed forest stand. Silv Genet 34: 241-247.
• Müller-Starck G, 1989. Genetic implications of environmental stress in adult forest stands of Fagus sylvatica L. In: Scholz F, Gregorius H-R, Rudin D, eds. Genetic aspects of air pollutants in forest tree populations. Springer Verlag, Berlin-Heidelberg: 127-142.
• Muona O, Szmidt AE, 1985. A multilocus study of natural populations of Pinus sylvestris. Lect Notes Biomath 60: 226-240.
• Oleksyn J, Chałupka W, Tjoelker MG, Reich PB, 1992. Geographic origin of Pinus sylvestris populations influences the effects of air pollution on flowering and growth. Water Air Soil Pollut 62: 201-212.
• Oleksyn J, Prus-Głowacki W, Giertych M, Reich PB, 1994. Relation between genetic diversity and pollution impact in a 1912 experiment with East European Pinus sylvestris provenances. Can J Forest Res 24: 2390-2394.
• Pahlsson AMB, 1990. Toxicity of heavy metals (Zn, Cu, Cd, Pb) to vascular plants. Water Air Soil Pollut 47: 287-319.
• Powell MJ, Davies MS, Francis D, 1986. The influence of zinc on the cell cycle in the root meristem of a zinc-tolerant and non-tolerant cultivar of Festuca rubra L. New Phytologist 104: 671-679.
• Prus-Glowacki W, Godzik St, 1991. Changes induced by zinc smelter pollution in the genetic structure of pine (Pinus sylvestris L.) seedling populations. Silv Genet 40: 184-188.
• Prus-Głowacki W, Nowak-Bzowy R, 1992. Genetic structure of naturally regenerating Scots pine population tolerant for high pollution near a zinc smelter. Water Air Soil Pollut 62: 249-259.
• Prus-Głowacki W, Oleksyn J, Reich PB, 1998. Relation between genetic structure and susceptibility to air pollution of European Pinus sylvestris populations from IUFRO-1982 provenance experiment. Chemosphere 4-5: 813-818.
• Presidential decrees of Minister of Environment (Journal of Laws of Republic of Poland 165, item 1359).
• Riegel R, Schubert R, Müller-Starck G, Karnosky D, Paule L, 2001. Genetic variation in two heavily polluted stands of Norway spruce (Picea abies [L.] Karst.) In: Müller-Starck, Schubert R, eds. Genetic response of forest system to changing environmental conditions. Kluwer Academic Publishers, Dordrecht, Boston, London: For Sci 70: 21-34.
• Rudin D, Ekberg I, 1978. Linkage studies in Pinus sylvestris using macrogametophyte allozymes. Silv Genet 27: 1-12.
• Starova NV, Kalashnik NA, Bakhtiyarova RM, 1994. Genetic diversity of coniferous species populations in mountain and plain forest, possible mechanism of genetic polymorphism. IUFRO S 2. Symposium Lithuania 13-17 September: 87-94.
• Staszak J, Grulke NE, 2004. Genetic differences of Pinus ponderosa [Dougl. ex Laws.] trees tolerant and sensitive to ozone. Water Air Soil Pollut 153: 3-14.
• Wawrzoniak J, 2002. Forest monitoring system in Poland. In: Siwecki R, ed. Biological reactions of trees to industrial pollution I, Sorus, Poznań: 67-81.
• Wonisch A, Tausz M, Muller M, Weindes W, De Kok LJ, Grill D, 1999. Treatment of young spruce shoots with SO₂ and H₂S effects on fine root chromosomes in relation to changes in the thiol content and redox state. Water Air Soil Pollut 116: 423-428.
• Wolf H, 2001. Effects ofextreme SO₂ pollution in winter 1995/96 on vitality and growth of SO2-tolerant Norway spruce (Picea abies [L.] Karst.) clones in the Ore mountains. In: Müller-Starck, Schubert R, eds. Genetic response of forest system to changing environmental conditions. Kluwer Academic Publishers, Dordrecht, Boston, London (For. Sci.) 70: 35-49.
• Yeh FC, Yang RC, Boyle T, 1999. Popgene, Version 1.31 Microsoft Windows based on Freeware for Population Genetic Analysis, University of Alberta.
• Zwoliński J, 2002. Indexes of forest decline caused by industrial pollutant deposition. In: Siwecki R, ed. Biological reactions of trees to industrial pollution I, Sorus, Poznań: 237-249.