Effects of Penicillium mycotoxins: citrinin, ochratoxin A, and patulin on in vitro proliferation of bovine lymphocytes

• Autorzy: Stec J , Rachubik J. , Szczotka M. , Kuzmak J.
• Języki publikacji: EN

Abstrakty

EN

In the presented study, ochratoxin A (OTA), patulin (PAT), and citrinit (CIT), three prevalent mycotoxins of Penicillium species, were evaluated for their response of bovine peripheral blood mononuclear cells (PBMC) stimulated in vitro by the mitogens: phytohaemagglutinin (PHA), concanavalin A (Con A), and pokeweed mitogen (PWM). The cultures of PBMC were assayed after 3-day incubation using ³H-thymidine uptake and the MTT bioassay. The concentrations of mycotoxins necessary to reduce the proliferatve response of PBMC by 50%, for PHA, Con A, and PWM as measured by ³H-thymidine incorporation were as follows: OTA - 0.35, 0.78, and 0.51 µg/ml; PAT - 0.12, 0.20, and 0.10 µg/ml; CIT - 1.46, 1.35, and 1.25 µg/ml, respectively. The concentration of mycotoxins causing 50% inhibition of PHA, Con A, and PWM stimulated PBMC proliferation by the MTT bioassay were for OTA - 1.69, 1.80, and 1.85 µg/ml; PAT - 0.64, 0.48, and 0.55 µg/ml; and CIT - 12.3, 10.04, and 10.9 µg/ml, respectively. According to this study, proliferative effect as measured by ³H-thymidine incorporation was more sensitive test than MTT bioassay and the PAT was the most potent inhibitor of PBMC proliferation while CIT was the less effective to the cultured cells.

Słowa kluczowe

EN

Penicillium; citrinin; peripheral blood mononuclear cell; cattle; mycotoxin; patulin; H-thymidine; lymphocyte; ochratoxin; proliferation

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 1
• Opis fizyczny: p.163-167,fig.,ref.

Twórcy

autor

Stec J

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Rachubik J.

autor

Szczotka M.

autor

Kuzmak J.

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