Identification of teratogens in embryo cell culture. I. Laboratory values of rat embryo limb buds

• Autorzy: Minta M
• Języki publikacji: EN

Abstrakty

EN

Embryonic cells, when cultured at high density, are referred to as „micromass” cultures and can be used as a tool to predict the teratogenic potential of xenobiotics in screen test. The rate of proliferation and differentiation can easily be measured quantitatively by simple staining methods with subsequent spectrophotometry. However, reproducibility of the results can be influenced by variability of the technique. To adapt this method in our laboratory systematic series of experiments with limb bud cell cultures were undertaken. Inter-relationships between different variables were checked.

Słowa kluczowe

EN

teratogenicity; limb bud; rat; cell culture; embryonic cell; micromass assay; embryo; identification

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2001
• Tom: 45
• Numer: 1
• Opis fizyczny: p.89-97,fig.

Twórcy

autor

Minta M

• National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

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