Wplyw produktow ekskrecyjno-sekrecyjnych Fasciola hepatica na funkcjonowanie hepatocytow szczurzych

• Autorzy: Gajewska A , Smaga-Kozlowska K. , Kotomski G.

Warianty tytułu

EN

Effect of excretory-secretory products of Fasciola hepatica on the functioning of rat hepatocytes

• Języki publikacji: PL

Abstrakty

EN

Fasciolosis, caused by Fasciola hepatica is a hepatic parasitic infection that affects numerous mammal species mainly ruminants, and is still a major veterinary problem in Poland causing huge economic losses in the cattle industry. The economic significance of fasciolosis is mainly due to direct losses caused by decrease in weight gain, milking capacity and the confiscation of altered livers in slaughterhouse. The molecular mechanisms of host-parasite interactions are still little known. The aim of the study was to investigate changes in the metabolic activity of freshly isolated rat hepatocytes exposed to excretory-secretor products (ESP) of Fasciola hepatica. The metabolic activity of isolated hepatocytes was determined with MTT and enzyme leakage tests. Hepatocytes were incubated with 0-1 mg/ml ES proteins from 2 to 72 hours. It was found that both the concentration and longevity of ESP action may hamper the metabolic activity of hepatocytes. Decrease in the metabolic activity of the cells correlated with an increase in enzyme leakage (ALT, ASPAT and LDH). Moreover, it was found that the level of albumin in the medium gradually decreased after 24 hrs of incubation with EPS, which may have resulted from an alternation of protein synthesis in the cells treated with the liver fluke metabolites. In conclusion, the results of the study indicate that ESP of Fasciola hepatica plays an important role in damaging liver cells of the host during invasions.

Słowa kluczowe

PL

produkty wydalniczo-wydzielnicze; Fasciola hepatica; motylica watrobowa; hepatocyty; uszkodzenia

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 04
• Opis fizyczny: s.459-462,rys.,bibliogr.

Twórcy

autor

Gajewska A

• Szkola Glowna Gospodarstwa Wiejskiego, ul.Ciszewskiego 8, 02-786 Warszawa

autor

Smaga-Kozlowska K.

autor

Kotomski G.