Distribution and immunohistochemical characterisation of paracervical neurons innervating the oviduct in the pig

• Autorzy: Czaja K , Wasowicz K. , Klimczuk M. , Podlasz P. , Lakomy M.
• Języki publikacji: EN

Abstrakty

EN

The present study was aimed at disclosing the distribution of paracervical neurons projecting to the ampulla and isthmus of the porcine oviduct and the pattern(s) of co-existence of tyrosine hydroxylase (TH), dopamine β-hydroxylase (DβH), neuropeptide Y (NPY), substance P (SP), calcitonin gene-related peptide (CGRP) and nitric oxide synthase (NOS) within these nerve cell bodies. The fluorescent retrograde tracer Fast Blue (FB) was injected into the wall of the ampullar (n = 3) and isthmal (n = 3) part of the organ in six sexually immature female pigs. After a survival period of three weeks paracervical ganglia (PCG) were collected. 10 µm-thick cryostat sections of the ganglia were examined for the presence of FB-positive (FB⁺) nerve cells under the fluorescent microscope. Tracered neurons were counted in every third section and processed for double-labelling immunofluorescence according to the method of Wessendorf and Elde. 78.6% of FB⁺ neurons were projecting to the isthmus while 21.4% of the studied population innervated the ampulla of the oviduct. Double-labelling immunofluorescence revealed the existence of the following different chemically coded subpopulations of the studied perikarya: TH⁺/DβH⁺, TH⁺/NPY⁺, TH⁺/NOS⁺, TH⁺/ NOS⁻, SP⁻/NOS⁺, SP⁺/CGRP⁺.

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2001
• Tom: 60
• Numer: 3
• Opis fizyczny: p.205-211,fig.,ref.

Twórcy

autor

Czaja K

• University of Warmia and Mazury in Olsztyn, Bldg. 105 J., Oczapowskiego 13, 10-917 Olsztyn-Kortowo II, Poland

autor

Wasowicz K.

autor

Klimczuk M.

autor

Podlasz P.

autor

Lakomy M.

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