Distinct roles for sphingolipids and glycosphingolipids at different stages of neuronal development

• Autorzy: Futerman A. H.
• Języki publikacji: EN

Abstrakty

EN

Studies on the roles of sphingolipids (SLs) and glycosphingolipids (GSLs) at distinct stages of neuronal development have been performed using primary cultures of hippocampal neurons, which are unique among neuronal cultures inasmuch as they develop by a well-characterized and stereotypic sequence of events that gives rise to fully differentiated axons and dendrites. Our data demonstrate that SLs and GSLs play at least three distinct roles in regulating neuronal development, namely: (i) ceramide enhances the formation of minor neuronal processes from lamellipodia and the subsequent stage of axonogenesis; (ii) glucosylceramide synthesis, but not the synthesis of higher-order GSLs, is required for normal axon growth and for accelerated axonal growth upon stimulation by growth factors; and (iii) at both of these stages, ceramide at high concentrations can induce apoptotic cell death. Together, these observations are consistent with the possibility that minor process formation and apoptosis are regulated by ceramide-dependent signaling pathways, whereas axonal growth requires glucosylceramide synthesis, perhaps to support an intracellular transport pathway.

Słowa kluczowe

EN

ceramide; glycosphingolipid; dendrite; endocytosis; axon; neuron; growth factor; sphingolipid; hippocampus

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 2
• Opis fizyczny: p.469-478,fig.

Twórcy

autor

Futerman A. H.

• Weizmann Institute of Science, Rehovot 76100, Israel

Bibliografia

• Bielawska, A., Crane, H.M., Liotta, D., Obeid, L.M. & Hannun, Y A. (1993) Selectivity of ceramide- mediated biology: Lack of activity of erythro- dihydroceranide. J. Biol Chem. 268, 26226- 26232.
• Boldin, S. & Futerman, A.H. (1997) Glucosylcera- mide synthesis is required for basic fibroblast growth factor and laminin to stimulate axonal growth. J. Neurochem. 68, 882-885.
• Chen, C.S., Rosenwald, A.G. & Pagano, R.E. (1995) Ceramide as a modulator of cndocyto- sis. J. Biol. Chem. 270, 13291-13297.
• Dobrowsky, R.T., Werner, M.H., Castellino, A.M., Chao, M.V. & Hannun, Y.A. (1994) Activation of the sphingomyelin cycle through the low- affinity neurotrophin receptor. Science 265, 1596-1599.
• Dotti, C.G., Sullivan, C.A. & Banker, G.A. (1988) The establishment of polarity by hippocampal neurons in culture. J. Neurosci. 8,1454-1468.
• Dotti, C.G., Parton, R.G. & Simone, K. (1991) Po larized sorting of glypiated proteins in hippo­campal neurons. Nature 349, 158-161.
• Fishbein, J.D., Dobrowsky, R.T., Bielawska, A., Garrett, S. & Hannun, Y.A. (1993) Ceramide- mediated growth inhibition and CAPP are con­served in Saccharomyces cerevisiae. J. Biol. Chem. 268, 9255-9261.
• Furuya, S., Hashikawa, T., Irie, F., Hasegawa, A., Nakao, T. & Hirabayashi, Y. (1995) Neuronal expression of a minor monosialosyl ganglio- side GMlb in rat brain: Immunochemical characterization using a specific monoclonal antibody. Neurosci. Res. 22, 411-421.
• Furuya, S., Hashikawa, T. & Hirabayashi, Y. (1996) Restricted occurrence of an unusual ganglioside GDI alpha in rat brain and its pos­sible involvement in dendritic growth of cere­bellar Purkinje neurons. J. Neurosci. Res. 44, 73-83.
• Futerman, A.H., Stieger, B., Hubbard, A.L. & Pa­gano, R.E. (1990) Sphingomyelin synthesis in rat liver occurs predominantly at the cis and medial cisternae of the Golgi apparatus. J. Biol Chem. 265, 8650-8657.
• Futerman, A.H. (1994a) Ceramide metabolism is compartmentalized in the endoplasmic reticu­lum and Golgi apparatus. Curr. Topics in Mem­branes 40, 93-110.
• Futerman, A.H. (1994b) An update on sphin- golipid synthesis and transport through the secretory pathway. Trends GlycoscL Glycotech- noL 6, 143-153.
• Futerman, A.H. & Banker, G.A. (1996) The eco­nomics of neurite growth — the addition of new membrane to the growing axon. Trends Neurosci. 19, 144-149.
• Goslin, K. & Banker, G. (1991) Rat hippocampal neurons in low density culture; in Culturing Nerve Cells (Banker, G. & Groslin, K., esd.) pp. 251-281, MIT Press, Cambridge.
• Hannun, Y.A. & Obeid, L.M. (1995) Ceramide: An intracellular signal for apoptosis. Trends Bio-chem. Sci. 20, 73-77.
• Harel, R. & Futerman, A.H. (1993) Inhibition of sphingolipid synthesis affects axonal out­growth in cultured hippocampal neurons. J. Biol Chem. 268, 14476-14481.
• Ilirschberg, K., Zisling, R., van Echten-Deckert, G. & Futerman, A.H. (1996) Ganglioside syn­thesis during the development of neuronal po­larity: major changes occur during axonogene- sis and axon elongation, but not during den­drite growth or during synaptogenesis. J. Biol. Chem. 271, 14876-14882.
• Ledeen, R.W. & Yu, G. (1992) Ganglioside func­tion in the neuron. Trends GlycoscL Glycotech- nol. 4, 174-187.
• Li, R.X. & Ladisch, S. (1997) Inhibition of endoge­nous ganglioside synthesis does not block neu- rite formatian by retinoic acid-treated neuro­blastoma cells. J. Biol. Chem. 272, 1349- 1354.
• Fushkareva, M., Obeid, L.M. & Hannun, Y.A. (1995) Ceramide: An endogenous regulator of apoptosis and growth suppression. Immunol Today 16, 294-297.
• Riboni, L., Prinetti, A., Bassi, R., Caminiti, A. & Tettamanti, G. (1995) A mediator role of cera- mide in the regulation of neuroblastoma Neu- ro2a cell differentiation. J. Biol Chem. 270, 26868-26875.
• Rosenwald, A.G., Machamer, C.E. & Pagano, R.E. (1992) Effects of a sphingolipid synthesis in­hibitor on membrane transport through the secretory pathway. Biochemistry 31, 3581- 3590.
• Rosenwald, A.G. & Pagano, R.E. (1993) Inhibition of glycoprotein traffic through the secretory pathway by ceramide J. Biol Chem. 268, 4577-4579.
• Schwarz, A., Rapaport, E.. Hirschberg, K. & Futerman, A.H. (1995) A regulatory role for sphingolipics in neuronal growth: Inhibition of sphingolipid synthesis and degradation have opposite effects on axonal branching. J. Biol Chem. 270, 10990-10998.
• Schwarz, A. & Futerman, A.H. (1996) The localiza­tion of gangliosides in neurons of the central nervous system: The use of anti-ganglioside antibodies Biochim. Biophys. Acta 1286, 247- 267.
• Schwarz, A. & Futerman, A.H. (1997a) Determina­tion of the localization of gangliosides using anti-ganglioside antibodies: Comparison of fixation methods J. Histochem. Cytochem. 45, 611-618.
• Schwarz, A. & Futerman, A.H. (1997b) Distinct roles for ceramide and glucosylceramide at dif­ferent stages of neuronal growth J. Neurosci. 17, 2929-2938.
• Siegel, D.A. & Walkley, S.U. (1994) Growth of ec­topic dendrites on cortical pyramidal neurons in neuronal storage diseases correlates with abnormal accumulation of GM2 ganglioside. J. Neurochem. 62, 1852-1862.
• Simons, K. & van Meer, G. (1988) Lipid sorting in epithelial cells. Biochemistry 27, 6197-6202.
• Sofer, A. & Futerman, A.H. (1995) Cationic amphi- philic drugs inhibit the internalization of chol­era toxin to the Golgi apparatus and the subse­quent elevation of cyclic AMP. J. Biol Chem. 270, 12117-12122.
• Sofer, A. & Futerman, A.H. (1996) Rate of retro­grade transport of cholera toxin from the plasma membrane to the Golgi apparatus and endoplasmic reticulum decreases during neu­ronal development. J. Neurochem. 67, 2134- 2140.
• Sofer, A., Schwarzmann, G. & Futerman, A.H. (1996) The internalization of a short acyl chain analogue of ganglioside GM(1) in polar­ized neurons. J. Cell Sci. 109, 2111-2119.
• Uemura, K., Sugiyama, E. & Taketomi, T. (1991) Effects of an inhibitor of glucosylceramide synthase on glycosphingolipid synthesis and neurite outgrowth in murine neuroblastoma cell lines. J. Biochem. (Tokyo) 110, 96-102.