GATA-1 binding to the alphaV promoter negatively regulates expression of the integrin alphaV subunit in human leukemic K562 cells

• Autorzy: Czyz M. , Stasiak M. , Boncela J. , Cierniewski C.S.
• Języki publikacji: EN

Abstrakty

EN

Recently we observed that the transcription factors Sp1 and Sp3 bind to the CTCCTCCTC sequence located between positions -194 and -172 of the αv promoter region and are directly involved in the regulation of transcriptional activity of the αv gene in human umbilical vascular endothelial cells (HUVECs) (Czyz & Cierniewski, 1999, Eur. J. Biochem. 265, 638). In this report we provide evidence that the GATA-1 factor regulates αv expression during differentiation of pluripotent K562 cells in­duced either by phorbol 12-myristate 13-acetate (PMA) or butyric acid (BA) through interaction with the GATA element in the «v gene promoter. DNase I footprinting analysis revealed that region -413 to -408, covering the GATA binding site, was pro­tected by nuclear extract from K562 cells. There was no protection of this region by HUVEC nuclear extract. Electrophoretic mobility shift assay (EMSA) analysis of nu­clear extract of K562 cells treated with BA revealed an increase in GATA binding ac­tivity, which was associated with reduced αv mRNA and αv protein on the cell surface. Stimulation of K562 cells with PMA resulted in opposite effects: lower expression of GATA-1 was associated with increased levels of αv. We conclude that the GATA-1 tran­scription factor specifically binds to the GATA element in the αv gene promoter and negatively regulates αv gene expression.

Słowa kluczowe

EN

transcription regulation; human umbilical vein endothelial cell; cell; gene expression; K562 cell; glyceraldehyde-3-phosphate dehydrogenase; integrin expression; polymerase chain reaction; phorbol myristate acetate

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 1
• Opis fizyczny: p.19-28,fig.

Twórcy

autor

Czyz M.

• Medical University of Lodz, W.Lindleya 6, 90-131 Lodz, Poland

autor

Stasiak M.

autor

Boncela J.

autor

Cierniewski C.S.

Bibliografia

• 1.Dubant, J.L., Rocher, S., Chen, W.-T., Yamada, K.M. & Thiery, J.P. (1986) Cell adhesion and migration in the early vertebrate embryo: Location and possible role of putative fibronectin receptor complex. J. Cell Biol. 102, 160-173.
• 2.Chen, W.T., Chen, J.M. & Mueller, S.C. (1986) Coupled expression and colocalization of 140K cell adhesion molecules, fibronectin, and laminin during morphogenesis and cytodifferentiation of chick lung cells. J. Cell Biol. 103, 1073-1090.
• 3.Vol, T., Fessler, L.I. & Fessler, J.H. (1990) A role for integrin in the formation of sarcomeric cytoarchitecture. Cell 63, 525-536.
• 4.Zutter, M.M., Fong, A.M., Krigman, H.R. & Santoro, S.A. (1992) Differential regulation of the alpha2 beta1 and alphaIIb beta3 integrin genes during megakaryocytic differentiation of pluripotential K562 cells. J. Biol. Chem. 267, 20233-20238.
• 5.Kitazawa, S., Ross, F.P., McHugh, K. & Teitelbaum, S.L. (1995) Interleukin-5 induces expression of the integrin alphaV beta3 via transactivation of the beta3 gene. J. Biol. Chem. 270, 4115-4120.
• 6.Zambruno, G., Marchisio, P. C., Marconi, A., Vaschieri, C., Melchiori, A., Giannetti, A. & De Luca, M. (1995) Transforming growth factor- beta1 modulates beta1 and beta5 integrin receptors and induces the de novo expression of the alphaV beta6 heterodimer in normal human keratinocytes: Implications for wound healing. J. Cell Biol. 129, 853-865.
• 7.Enenstein, J., Waleh, N.S. & Kramer, R.H. (1992) Basic FGF and TGF-beta differentially modulate integrin expression of human microvascular endothelial cells. Exp. Cell Res. 203, 499-503.
• 8.Hynes, R.O. (1992) Integrins: Versatility, modulation, and signalling in cell adhesion. Cell 69, 11-25.
• 9.Bates, R., Rankin, L., Lucas, S., Scott, J., Krissansen, G. & Burns, G. (1991) Individual embryonic fibroblasts express multiple beta chains in association with the alphaV integrin subunit. J. Biol. Chem. 266, 18593-18599.
• 10.Defilippi, P., Truffa, G., Stefanuto, G., Altruda, F., Silenge, L. & Tarone, G. (1991) Tumor necrosis factor alpha and interferon gamma modulate the expression of the vitronectin receptor (integrin beta3) in human endothelial cells. J. Biol. Chem. 266, 7638-7645.
• 11.Kim, L.T. & Yamada, K.M. (1997) The regulation of expression of integrin receptors. Proc. Soc. Exp. Biol. Med. 214, 123-131.
• 12.Medhora, M.M., Teitelbaum, S., Chappel, J., Alvarez, J., Mimura, H., Ross, F.P. & Hruska, K. (1993) 1alpha,25- dihydroxyvitamin D3 up-regulates expression of the osteoclast integrin alpha v beta 3. J. Biol. Chem. 268, 1456-1461.
• 13. Donahue, J.P., Sugg, N. & Hawiger, J. (1994) The integrin alphaV gene: Identification and characterisation of the promoter region. Biochim. Biophys. Acta 1219, 228-232.
• 14.Vila-Garcia, M., Li, L., Riely, G. & Bray, P.F. (1994) Isolation and characterisation of a TATA-less promoter for the human beta3 integrin gene. Blood 83, 668-676.
• 15.Czyz, M. & Cierniewski, C.S. (1999) Selective Sp1 and Sp3 binding is crucial for activity of the integrin alphaV promoter in cultured endothelial cells. Eur. J. Biochem. 265, 638-644.
• 16.Kim, L.T., Wu, J. & Turnage, R.H. (1998) Integrin alphaV promoter activity in keratinocytes. J. Surg. Res. 76, 185-191.
• 17.Dai, W. & Murphy, M.J., Jr. (1993) Downregulation of GATA-1 expression during phorbol myristate acetate-induced megakaryocyte differentiation of human erythroleukemia cells. Blood 81, 1214-1221.
• 18.Chenais, B., Molle, I., Trentesaux, C. & Jeannesson, P. (1997) Time-course of butyric acid-induced differentiation in human K562 leukemic cell line: Rapid increase in gamma-globin, porphobilinogen deaminase and NF-E2 mRNA levels. Leukemia 11, 1575-1579.
• 19.Chenais, B. (1998) Requirement of GATA-1 and p45 NF-E2 expression in butyric acid-induced erythroid differentiation. Biochem. Biophys. Res. Commun. 253, 883-886.
• 20.Shimamoto, T., Ohyashiki, J.H., Ohyashiki, K. & Toyama, K. (1995) The expression pattern of transcription factors (GATA, SCL) and biological characteristics in various leukemia cells. Rinsho-Ketsueki. 36, 547-551.
• 21.Dignam, J.D., Martin, P.L. & Roeder, R.G. (1983) Eukaryotic gene transcription with purified factors. Methods Enzymol. 101, 582-598.
• 22.Moll, T., Czyz, M., Holzmuller, H., Hofer- Warbinek, R., Wagner, E., Winkler, H., Bach, F.H. & Hofer, E. (1995) Regulation of the tissue factor promoter in endothelial cells. J. Biol. Chem. 270, 3849-3857.
• 23.Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of micrograms quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
• 24.Kambe, M., Miyamoto, Y. & Hayashi, M. (1998) Characterization of the integrin alphaV gene promoter in mice. Biochim. Biophys. Acta 1395, 209-219.
• 25.Feng, X., Teitelbaum, S.L., Quiroz, M.E., Cheng, S.-L., Lai, C.-F., Avioli, L.V. & Ross, F.P. (2000) Sp1/Sp3 and PU.1 differentially regulate beta5 integrin gene expression in macrophages and osteoblasts. J. Biol. Chem. 275, 8331-8340.
• 26.Noti, J.D., Johnson, A.K. & Dillon, J.D. (2000) Structural and functional characterization of the leukocyte integrin gene CD11d. J. Biol. Chem. 275, 8959-8969.
• 27. Li, J., Noguchi, C.T., Miller, W., Hardison, R. & Schechter, A.N. (1998) Multiple regulatory elements in the 5'- flanking sequence of the human epsilon-globin gene. J. Biol. Chem.. 273, 10202-10209.
• 28.Amrolia, P.J., Cunningham, J.M., Ney, P., Nienhuis, A.W. & Jane, S.M. (1995) Identification of two novel regulatory elements within the 5'-untranslated region of the human A gamma -globin gene. J. Biol. Chem. 270, 12892-12898.
• 29.Chin, K., Oda, N., Shen, K. & Noguchi, C.T. (1995) Regulation of transcription of the human erythropoietin receptor gene by proteins binding to GATA-1 and Sp1 motifs. Nucleic Acids Res. 23, 3041-3049.
• 30. Imagawa, S., Yamamoto, M. & Miura, Y. (1997) Negative regulation of the erythropoietin gene expression by the GATA transcription factors. Blood 89, 1430-1439.