Nickel impairs the repair of UV - and MNNG-damaged DNA

• Autorzy: Wozniak K , Blasiak J.
• Języki publikacji: EN

Abstrakty

EN

Nickel(II) is reported to be genotoxic, but the mechanisms underlying its genotoxicity are largely unknown. It can interfere with DNA repair and this may contribute to its genotoxicity. We studied the effect of nickel chloride on the repair of DNA damaged by UV radiation or N-methyl-N-nitro-N-nitrosoguanidine (MNNG) in human lymphocytes using the alkaline comet assay. Nickel(II) at 1 μM caused an accumulation of DNA breaks during repair incubation, which could follow from the inhibition of the polymerization/ligation step of UV-damaged DNA repair. On the other hand, nickel(II) inhibited the formation of transient DNA breaks brought by the repair process after incubation with MNNG at 5 μM, which might follow from interference with the recognition/incision step of excision repair. Additionally, nickel at 1 μM inhibited the activity of formamidopyrimidine-DNA glycosylase (Fpg) and 3-methyladenine-DNA glycosylase II (Alk A), enzymes involved in DNA excision repair. A decrease in endonuclease III (Endo III) activity was observed at 2 and 5 μM of nickel chloride. Our results suggest that nickel(II) at non-cytotoxic concentrations can inhibit various steps of DNA excision repair, and this may contribute to its genotoxicity.

Słowa kluczowe

EN

ultraviolet light; genotoxicity; lymphocyte; DNA repair; DNA damage; nickel chloride; nickel

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 1
• Opis fizyczny: p.83-94,fig.,ref.

Twórcy

autor

Wozniak K

• University of Lodz, Banacha 12-16, 90-237 Lodz, Poland

autor

Blasiak J.

Bibliografia

• 1. IARC, Monographs on the Evaluation of Carcinogenic Risks in Humans, Chromium, Nickel, and Welding, vol. 49, World Health Organization and International Agency for Research on Cancer, Lyon, France, 1990, 257-411.
• 2. Misra, M., Oliński, R., Dizdaroglu, M. and Kasprzak, K.S. Enhancement of L-histidine of nickel(II)-induced DNA-protein croos-links and oxidative DNA base damage in the rat kidney. Chem. Res. Toxicol. 6 (1993) 33-37.
• 3. Werfel, U., Langen, V., Eickhoff, I., Schoonbrood, J., Vahrenholz, C., Brauksiepe A., Popp, W. and Norpoth, K. Elevated DNA single-strand breakage frequencies in lymphocytes of welders exposed to chromium and nickel. Carcinogenesis 19 (1998) 413-418.
• 4. Chakrabarti, S.K., Bai, C. and Subramanian, K.S. DNA-protein cross-links induced by nickel compounds in isolated rat lymphocytes: role of reactive oxygen species and specific amino acids. Toxicol. Appl. Pharmacol. 170 (2001) 153-165.
• 5. Kawanishi, S., Inoue, S., Oikawa, S., Yamashita, N., Toyokuni, S., Kawanishi, M. and Nishino, K. Oxidative DNA damage in cultured cells and rat lungs by carcinogenic nickel. Free Radic. Biol. Med. 31 (2001) 108-116.
• 6. Chen, C.Y., Wang, Y.F., Huang, W.R. and Huang, Y.T. Nickel induces oxidative stress and genotoxicity in human lymphocytes. Toxicol. Appl. Pharmacol. 189 (2003) 153-159.
• 7. Woźniak, K. and Błasiak, J. Free radicals-mediated induction of oxidized DNA bases and DNA-protein cross-links by nickel chloride. Mutat. Res. 514 (2002) 233-243.
• 8. Klein, C.B., Conway, K., Wang, X. W., Bhamra, R.K., Lin, X., Cohen, M. D., Annab, L., Barrett, J.C. and Costa, M. Senescence of nickel-transformed cells by an X chromosome: possible epigenetic control. Science 251 (1991) 796-799.
• 9. Lee, Y-W., Klein, C.B., Kargacin, B., Salnikow, K., Kitahara, J., Dowjat, K., Zhitkovich, A., Christie, N.T. and Costa, M. Carcinogenic nickel silences gene expression by chromatin condensation and DNA methylation: a new model for epigenetic carcinogens. Mol. Cell. Biol. 15 (1995) 2547-2557.
• 10. Denkhaus, E. and Salnikow, K. Nickel essentiality, toxicity and carcinogenicity. Crit. Rev. Oncol. Hematol. 42 (2002) 35-56.
• 11. Hartwig, A. Carcinogenicity of metal compounds: possible role of DNA repair inhibition. Toxicol. Lett. 102 (1998) 235-239.
• 12. Hartwig, A. and Schwerdtle, T. Interactions by carcinogenic metal compounds with DNA repair processes: toxicological implications. Toxicol. Lett. 127 (2002) 47-54.
• 13. Lee-Chen, S.F., Wang, M.C., Yu, C.T., Wu, D.R. and Jan, K.Y. Nickel chloride inhibits the DNA repair of UV-treated but not methyl methanesulfonate-treated Chinese hamster ovary cells. Biol. Trace Elem. Res. 37 (1993) 39-50.
• 14. Au, W.W., Heo, M.Y. and Chiewchanwit, T. Toxicological interactions between nickel and radiation on chromosome damage and repair. Environ. Health Perspect 102 (1994) 73-77.
• 15. Dally, H. and Hartwig, A. (1997) Induction and repair inhibition of oxidative DNA damage by nickel(II) and cadmium(II) in mammalian cells. Carcinogenesis 18 (1997) 1021-1026.
• 16. Hartmann, M. and Hartwig, A. Disturbance of DNA damage recognition after UV-irradiation by nickel(II) and cadmium(II) in mammalian cells. Carcinogenesis 19 (1998) 617-621.
• 17. Takahashi, S., Takeda, E., Kubota, Y. and Okayasu, R. Inhibition of repair of radiation-induced DNA double-strand breaks by nickel and arsenite. Radiat. Res. 154 (2000) 686-691.
• 18. Schwerdtle, T., Seidel, A. and Hartwig, A. Effect of soluble and pariculate nickel compounds on the formation and repair of stable benzo[a]pyrene DNA adducts in human lung cells. Carcinogenesis 23 (2002) 47-53.
• 19. Krokan, H.E., Standal, R. and Slupphaug, G. DNA glycosylases in the base excision repair. Biochem. J. 325 (1997) 1-16.
• 20. Evans, M.D., Podmore, I.D., Daly, G.J., Perrett, D., Lunec, J. and Herbert, K.E. Detection of purine lesions in cellular DNA using single cell gel electrophoresis with Fpg protein. Biochem. Soc. Trans. 23 (1995) 434S.
• 21. Collins, A.R., Duthie, S.J. and Dobson, V.L. Direct enzymatic detection of endogenous base damage in human lymphocyte DNA. Carcinogenesis 14 (1993) 1733-1735.
• 22. Singh, N.P., McCoy, T., Tice, R.R. and Schneider, E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175 (1988) 184-192.
• 23. Klaude, M., Eriksson, S., Nygren, J. and Ahnstrom, G. The comet assay: mechanisms and technical consideration. Mutat. Res. 363 (1996) 89-96.
• 24. Błasiak, J. and Kowalik, J. A comparison of the in vitro genotoxicity of tri- and hexavalent chromium. Mutat. Res. 469 (2000) 135-145.
• 25. Hartwig, A. and Beyersmann, D. Enhancement of UV-induced mutagenesis and sister-chromatid exchanges by nickel ions in V79 cells: evidence for inhibition of DNA repair. Mutat. Res. 217 (1989) 65-73.
• 26. Krueger, I., Mullenders, L.H.F. and Harwig A. Nickel(II) increases the sensitivity of V79 Chinese hamster cells towards cisplatin and transplatin by interference with distinct steps of DNA repair. Carcinogenesis 20 (1999) 1177-1184.
• 27. Iwitzki, F., Schlepegrell, R., Eichhorn, U., Kaina, B., Beyersmann, D. and Hartwig, A. Nickel(II) inhibits the repair of O6-methylguanine in mammalian cells. Arch. Toxicol. 72 (1998) 681-689.
• 28. Asmuss, M., Mullenders, L.H.F., Eker, A. and Hartwig, A. Differential effects of toxic metal compounds on the activities of Fpg and XPA, two zinc finger proteins involved in DNA repair. Carcinogenesis 21. (2000) 2097-2104.
• 29. Lynn, S., Yew, F.H., Chen, K.S. and Jan, K.Y. Reactive oxygen species are involved in nickel inhibition of DNA repair. Environ. Mol. Mutagen. 29 (1997) 208-216.
• 30. Bal, W., Schwerdtle, T. and Hartwig, A. Mechanism of nickel assault on the zinc finger of DNA repair protein XPA. Chem. Res. Toxicol. 16 (2003) 242-248.
• 31. Kielbassa, C., Roza, L. and Epe, B. Wavelength dependence of oxidative DNA damage induced UV and visible light. Carcinogenesis 18 (1997) 811-816.
• 32. Bernstein, C., Bernstein, H., Payne, C.M. and Garewal, H. DNA repair/pro- apoptotic dual-role proteins in five major DNA repair pathways: fail-safe protection against carcinogenesis. Mutat. Res. 511 (2002) 145-178.
• 33. Snyder, R.D., Van Houten, B. and Regan, J.D. The accumulation of DNA strand breaks due to incision; comparative studies with various inhibitors, in: DNA repair and its inhibitors, (Collins, A., Downes, C. S. and Johnson, R. T., Ed.), IRL, Oxford, UK, 1984, 13-32.
• 34. Lindhal, T., Sedgwick, B., Sekiguchi, M. and Nakabeppu, Y. Regulation and expression of the adaptive response to alkylating agents. Annu. Rev. Biochem. 57 (1988) 133-157.
• 35. Asahina, H., Kuraoka, I., Shirakawa, M., Morita, E.H., Miura, N., Miyamoto, I., Ohtsuka, E., Okada, Y. and Tanaka, K. The XPA protein is a zinc metalloprotein with an ability to recognize various kinds of DNA damage. Mutat. Res. 315 (1994) 229-237.
• 36. Shi, X., Dalal, N.S. and Kasprzak, K.S. Generation of free radicals in reactions of Ni(II)-thiol complexes with molecular oxygen and model lipid hydroperoxides. J. Inorg. Biochem. 15 (1993) 211-225.