Differentiation of adventitious buds from Capsicum annuum L. hypocotyls after co-culture with Agrobacterium tumefaciens

• Autorzy: Delis M , Garbaczewska G. , Niemirowicz-Szczytt K.
• Języki publikacji: EN

Abstrakty

EN

In vitro organogenesis in hypocotyl explants of the pepper cultivar ‘Bryza’ was induced on MS medium containing 5 mg/l 6-benzyloaminopurine (BAP) and 1 mg/l indole-3-acetic acid (IAA). The hypocotyl explants were then inoculated with Agrobacterium tumefaciens LBA4404(pBI121). After 2 days of culture the first cell divisions were observed in the epidermis and cortex. After 6-7 days, numerous adventitious bud primordia appeared in 58.4% of the explants. In further stages of culture, buds developed into shoots in 8.4% of the explants. Histological analysis revealed hypertrophy and the presence of necrotic cells in the cortex. Necrotic changes were also observed in the vascular bundles. It is likely that culture on a selective medium containing kanamycin and co-culture with Agrobacterium tumefaciens strongly affected the organization of the hypocotyl meristematic tissue, and in consequence brought about necrosis and isolation of the adventitious buds from the vascular bundles.

Słowa kluczowe

EN

genetic transformation; Capsicum annuum; pepper; Agrobacterium tumefaciens; kanamycin; adventitious bud; anatomy; plant genetics; hypocotyl; bud

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2005
• Tom: 47
• Numer: 1
• Opis fizyczny: p.193-198,fig.,ref.

Twórcy

autor

Delis M

• Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warsaw, Poland

autor

Garbaczewska G.

autor

Niemirowicz-Szczytt K.

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