The composition of cell wall skeleton and outermost lipids of Mycobacterium vaccae is modified by ethambutol treatment

• Autorzy: Rumijowska-Galewicz A , Korycka-Machala M. , Lisowska K. , Dziadek J.
• Języki publikacji: EN

Abstrakty

EN

Ethambutol (EMB) is a first line drug in tuberculosis treatment inhibiting the biosynthesis of arabinogalactan, which is a component of the mycobacterial cell wall. The growth of Mycobacterium vaccae cells in the presence of EMB increases cell wall permeability, which was monitored by β-sitosterol biotransformation. GC/MS and GLC/MS (gas chromatography/mass spectrometry) analysis revealed dramatic changes in the content of covalently bound mycolic acids and in molar ratio galactose (Gal) to arabinose (Ara) in the cell envelopes of EMB-treated cells. The detected variations in the compositions of fatty acids indicate that both the cell wall skeleton and outer layer (free lipids) are decomposed due to EMB treatment.

Słowa kluczowe

EN

permeability; treatment; ethambutol; cell wall skeleton; Mycobacterium vaccae; mycobacterial cell

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2008
• Tom: 57
• Numer: 2
• Opis fizyczny: p.99-104,fig.,ref.

Twórcy

autor

Rumijowska-Galewicz A

• Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland

autor

Korycka-Machala M.

autor

Lisowska K.

autor

Dziadek J.

Bibliografia

• Azuma I., E.E. Ribi, T.J. Meyer and B. Zbar. 1974. Biologically active components from mycobacterial cell walls. I. Isolation and composition of cell wall skeleton and component P 3. J. Natl. Cancer Inst. 52: 95-101.
• Belanger A.E., G.S. Besra, M.E. Ford, K. Mikusova, J.T. Belisle, P.J. Brennan and J.M. Inamine. 1996. The embAB genes of Mycobacterium avium encode an arabinosyl transferase involved in cell wall arabinan biosynthesis that is the target for the antimycobacterial drug ethambutol. Proc. Natl. Acad. Sci. USA 93: 11919-11924.
• Brennan P.J. and H. Nikaido. 1995. The envelope of mycobacteria. Annu. Rev. Biochem. 64: 29-63.
• Connel N.D. and H. Nikaido. 1994. Membrane permeability and transport in Mycobacterium tuberculosis, pp 333-352. In: Bloom B.R. (ed). Tuberculosis Pathogenesis, Protection and Control, AFM Press, Washington, D C.
• Crick D.C., S. Mahapatra and P.J. Brennan. 2001. Biosynthesis of arabinogalactan-peptidoglycan complex of Mycobacterium tuberculosis. Glycobiology 11: 107-118.
• Daffe M. and P. Draper. 1998. The envelope layers of mycobacteria with reference to their pathogenicity. Adv. Microbiol. Physiol. 39: 131-203.
• David H.L., N. Rastogi, S. Clavel-Seres and F. Clement. 1988. Alteration in the outer wall architecture caused by the inhibition of mycoside C biosynthesis in Mycobacterium avium. Curt: Microbiol. 17: 61-68.
• Deng L., K. Mikusova, K.G. Robuck, M. Scherman, P.J. Brennan and M.R. McNeil. 1995. Recognition of multiple effects of ehtambutol on metabolism of mycobacterial cell envelope. Antimicrob. Agents Chemother. 39: 694-701.
• Gamian A., H. Mordarska, I. Ekiel, J. Ulrich, B. Szponar and J. Defaye. 1996. Structural studies of the major glycolipid from Saccharopolyspora genus. Carbohyd. Res. 296: 55-67.
• Garcia-Borcelo M., M. Luquin, F. Belda and V. Ausina. 1993. Gas chromatographic whole-cell fatty acid analysis as an aid for the identification of mixed mycobacterial cultures. J. Chrom. 617: 299-303.
• Häusler H., R.P. Kawakami, E. Mlaker, W.B. Severn and A.E. Stütz. 2001. Ethambutol analogues as potential antimycobacterial agents. Bioorg. Med. Chem. Letters 11: 1679-1681.
• Hunter S.W., M. McNeil, R.L. Modlin, V. Mehra, B.R. Bloom and P.J. Brennan. 1989. Isolation and characterisation of the highly immunogenic cell wall-associated protein of Mycobacterium leprae. J. Immun. 142: 2864-2872.
• Jackson M., C. Raynaud, M.A. Laneelle, Ch. Guilhot, Ch. Laurent-Winter, D. Ensergueix, B. Gicquel and M. Daffe. 1999. Inactivation of the antigen 85C gene profoundly affects the mycolate content and alters the permeability of the Mycobacterium tuberculosis cell envelope. Mol. Microbiol. 31: 1573-1587.
• Jarlier V. and H. Nikaido. 1990. Permeability barrier to hydrofilic solutes in Mycobacterium chelonae. J. Bacterial. 172: 1418-1423.
• Korycka-Machała M., A. Ziółkowski, A. Rumijowska-Galewicz, K. Lisowska and L. Sedlaczek. 2001. Polycations increase the permeability of Mycobacterium vaccae cell envelopes to hydrophobic compounds. Microbiology 147: 2769-2781.
• Korycka-Machała M., A. Rumijowska-Galewicz and J. Dziadek. 2005. The effect of ethambutol on the mycobacterial cell wall permeability to hydrophobic compounds. Pol. J. Microbiol. 54: 5-11.
• Koul A. and R. Prasad. 1996. Extraction of membrane lipids, pp. 36-51. In Prasad R. (ed). Manual on Membrane Lipids. Springer - Verlag Berlin Heidelberg.
• Lisowska K., M. Korycka, O. Hadław-Klimaszewska, A. Ziółkowski and L. Sedlaczek. 1996. Permeability of mycobacterial cell envelopes to sterols: peptidoglycan as the diffusion barrier. J. Basic Microbiol. 36: 407-419.
• Liu J., C.E. Barry, G.S. Besra and H. Nikaido. 1996. Mycolic acid structure determines the fluidity of the mycobacterial cell wall. J. Biol. Chem. 271: 29545-29551.
• Liu J., E.Y. Rosenberg and H. Nikaido. 1995. Fluidity of the lipid domain of cell wall from Mycobacterium chelonae. Proc. Natl. Acad. Sci. USA 92: 11254-11258.
• McNeil M. 1999. Arabinogalactan in mycobacteria: structure, biosynthesis and genetics, pp 207-223. In: Goldberg J.B. (ed). Genetics of Bacterial Polysaccharides, CRC Press, Washington, DC.
• Minnikin D.E., G. Hutchinson, A.B. Caldicott and M.Goodfellow. 1980. Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J. Chrom. 188: 221-233.
• Nikaido H. and V. Jarlier. 1991. Permeability of the mycobacterial cell wall. Res. Microbiol. 142: 437-443.
• Nikaido H., S.H. Kim and E.Y. Rosenberg. 1993. Physical organization of lipids in the cell wall of Mycobacterium chelonae. Mol. Microbiol. 8: 1025-1030.
• Pathak A.K., V. Pathak, J.M. Riordan, S.S. Gurcha, G.S. Besra and R.C. Reynolds. 2004. Synthesis of mannopyranose disac-charides as photoaffinity probes for mannosyltransferases in Mycobacterium tuberculosis. Carboh. Res. 339: 683-691.
• Puech V., N. Bayan, K. Salim, G. Leblan and M. Daffe. 2000 Characterization of the in vivo acceptors of the mycoloyl residues transferred by the corynebacterial PSI and the related mycobacterial antigen 85. Mol. Microbiol. 35: 1026-1041.
• Radmacher E., K.C. Stansen, G.S. Besra, L.J. Alderwick, W.N. Maughan, G. Hollweg, H. Sahm, V.F. Wendisch and L. Eggeling. 2005. Ethambutol, a cell wall inhibitor of Mycobacterium tuberculosis, elicits L-glutamate efflux of Corynebacterium glutamicum. Microbiology 151: 1359-68.
• Ramalho T.C., E.F..F da Cunha and R.B. de Alencastro. 2004. A density functional study on the complexation of ethambutol with divalent cations. J. Mol. Struct. Theochem. 676: 149-153.
• Ramaswamy S. and J.M. Musser. 1998. Molecular genetic basic of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuberc. Lung Dis. 79: 3-9.
• Rastogi N. 1991. Recent observations concerning structure and function relationships in the mycobacterial cell envelope: elaboration of a model in terms of mycobacterial pathogenicity, virulence and drug-resistance. Res. Microbiol. 142: 464-476.
• Rumijowska A., K. Lisowska, A. Ziółkowski and L. Sedlaczek. 1997. Transformation of sterols by Mycobacterium vaccae: effect of lecithin on the permeability of cell envelopes to sterols. World J. Microbiol. Biotechnol. 13: 89-95.
• Rumijowska-Galewicz A., A. Ziółkowski, M. Korycka-Machała and L. Sedlaczek. 2000. Alterations in lipid composition of Mycobacterium vaccae cell wall outer layer enhance β-sitosterol degradation. World J. Biol. Biotechnol. 16: 237-244.
• Sedlaczek L., B.M. Górmiński and K. Lisowska. 1994. Effect of inhibitors of cell envelope synthesis on β-sitosterol side chain degradation by Mycobacterium sp. NRRL 3683. J. Basic Microbiol. 34: 387-399.
• Sedlaczek L., K. Lisowska, M. Korycka, A. Rumijowska, A. Ziółkowski and J. Długoński. 1999. The effect of cell wall components on glycine-enhanced sterol side chain degradation to androstene derivatives by mycobacteria. Appl. Microbiol. Biotechnol. 52: 563-571.
• Sirgel F.A., P.R. Donald, J. Odhiambo, W. Githui, K.C. Umapathy, C.N. Paramasivan, C.M. Tam, K.M. Kam, C.W. Lam, K.M. Sole, D.A. Mitchison and others. 2000. A multicentre study of the earlysbactericidal activity of anti-tuberculosis drugs. J. Antimicrob. Chemother. 45: 859-870.
• Szentirmai A. 1990. Microbial physiology of side chain degradation of sterols. J. Ind. Microbiol. 6:101-115.
• Takayama K. and J.O. Kilburn. 1989. Inhibition of synthesis of arabinogalactan by ethambutol in Mycobacterium smegmutis. Antimicrob. Agents Chemother. 33: 1493-1499.
• Valero-Guillen PL. and F. Martín-Luengo. 1986. 1-Tetradecanol, a new alcohol found in the cell wall of some rapidly growing chromogenie mycobacteria. FEMS Microbiol. Letters 35: 59-63.
• Xin Y., Y. Huang and M.R. McNeil. 1999. The presence of an endogenous endo-D-arabinase in Mycobacterium smegmatis and characterization of its oligoarabinoside product. Biochim. Biophvs. Acta 1473: 267-271.