Genetic analysis of Polish isolate of swine vesicular disease virus [SVDV POL-73] by RT-PCR and nucleotide sequencing of the VP1-2A coding region

• Autorzy: Niedbalski W
• Języki publikacji: EN

Abstrakty

EN

A nucleotide sequence analysis by RT-PCR and cycle sequencing of the Polish isolate of swine vesicular disease virus (SVDV) was performed. The RT-PCR products pattern and VP1/2A coding region sequence were compared to the other available European isolates of SVDV and the human Coxackie virus isolate CAV-16. The results of the RT-PCR show that it is possible to detect different isolates of the SVDV however Coxackie virus-specific PCR products were obtained only by the use of one from three selected primer pairs. A sensitivity of the used RT-PCR was equivalent to that of virus isolation in cell culture and even higher than the ELISA method. The considerable nucleotide similarity between the Polish isolate UKG27/72 and the Japan 76 SVDV isolates (99% and 98%, respectively) as well as the CAV-16 (96%) was found. SVDV Poland 73 was genetically less related (about 88%) to the Dutch and Italian isolates from the recent outbreaks of disease in 1992. Presented results confirmed the quasispecies character of these picornaviruses.

Słowa kluczowe

EN

pig; Picornaviridae; disease; Enterovirus; virus; coding region; ELISA test; Polish isolate; swine vesicular disease; cell culture; virus isolate; nucleotide sequence

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1998
• Tom: 42
• Numer: 1
• Opis fizyczny: p.3-10,fig.

Twórcy

autor

Niedbalski W

• National Veterinary Research Institute, 98-220 Zdunska Wola, Poland

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