Koniugat CENTOCOR FITC w diagnostyce wscieklizny. I. Badania myszek zakazonych szczepem Flury LEP

• Autorzy: Zmudzinski J F , Smreczak M. , Skulmowska-Kryszkowska D. , Rola J.
• Języki publikacji: PL

Abstrakty

EN

The monoclonal CENTOCOR FITC conjugate produced by CENTOCOR, Malvern PA 19355, USA has been used for detection of rabies virus in brain impression slides from mice inoculated with Flury LEP strain MICLD₅₀ titer log 5,32. The conjugate has been used at the dilutions 1:10, 1:20, 1:40. For comparison same brain impression slides have been treated with anti-rabies policlonal conjugate made by czechoslovakian company Bioveta (working dilution 1:8). The control slides have comprised the impressions of normal (non-inoculated with rabies) mouse brain labeled with either CENTOCOR undiluted or Bioveta conjugate diluted 1:8. The same complexes emiting bright apple green fluorescence have been found out in impressions labeled with either Centocor or Bioveta conjugates. The CENTOCOR has given the positive reaction in the dilution up to 1:20. The background staining has been markedly reduced in impressions treated with CENTOCOR (contains Evans blue counterstain). The CENTOCOR lot No. 9C0011 may be used in the dilution 1:2 to provide 10-fold excess of antibody recommended by the company.

Słowa kluczowe

PL

choroby wirusowe; wscieklizna; koniugat CENTOCOR FITC; test immunofluorescencji; choroby zwierzat; diagnostyka laboratoryjna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 1993
• Tom: 49
• Numer: 01
• Opis fizyczny: s.19-21,fot.,bibliogr.

Twórcy

autor

Zmudzinski J F

• Instytut Weterynarii, Al.Partyzantow 57, 24-100 Pulawy

autor

Smreczak M.

autor

Skulmowska-Kryszkowska D.

autor

Rola J.

Bibliografia

• 1. Bourny H. Sureau Р.: Methodes de Laboratoire pour le Diagnostic de la Rage. Institut Pasteur, Rabies Unit, National Reference Centre for Rabies, WHO Collaborating Centre for Rabies, Paris, 1991.
• 2. Debbie J. G., Andrulonis J. A., Abelseth M. K.: Infect. Immun. 5, 902, 1972.
• 3. Kaplan M. M., Koprowski H.: Laboratory Techniques in Rabies, WHO Geneva, 1973.
• 4. McQueen J. L., Lewis A. L., Schneider N. J.: Am. J. publ. Hlth 50, 1743, 1960.
• 5. Wiktor T. J., Flamand A., Koprowski H.: J. Virol. Methods 1, 33 1980
• 6. Instrukcja Nr 27 Min. Rol. Dep. Wet. z dn. 16.04.1973 (Wet-L-640/1/73).