Signal transducer and activator of transcription STAT3 plays a major role in gp130-mediated acute phase protein gene activation

• Autorzy: May P. , Schniertshauer U. , Gerhartz C. , Horn F. , Heinrich P.C.
• Języki publikacji: EN

Abstrakty

EN

Interleukin-6 is a potent inducer of acute-phase response gene transcription. The intracellular signal transduction mechanisms by which this and other biological ef­fects of the cytokine are achieved include activation of the JAK-STAT signaling path­way. More specifically, activation of the signal transducers and activators of transcription STAT1, 3, and 5 in response to IL-6 has been described. We examined the relative potency of these three STAT factors for the activation of acute-phase gene promoters in HepG2 cells in a reporter gene-based assay, where spe­cific STAT factors could be activated via recombinant receptor constructs bearing dif­ferent STAT-recruiting modules. These experiments indicate that amongst the STAT factors known to be activated by IL-6 STAT3 is the most potent activator of acute-phase gene transcription.

Słowa kluczowe

EN

interleukin 6; gene; cytokine; signal transduction; gene transcription; protein; intracellular signal

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 3
• Opis fizyczny: p.595-601,fig.

Twórcy

autor

May P.

• UTSW Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9046, USA

autor

Schniertshauer U.

autor

Gerhartz C.

autor

Horn F.

autor

Heinrich P.C.

Bibliografia

• Akira S, Taga T, Kishimoto T. (1993) Interleukin-6 in biology and medicine. AdvImmunol.; 54: 1-78.
• Alonzi T, Maritano D, Gorgoni B, Rizzuto G, Libert C, Poli V. (2001) Essential role of STAT3 in the control of the acute- phase response as revealed by inducible gene inactivation [correction of activation] in the liver. Mol Cell Biol. ; 21: 1621-32.
• Arcone R, Pucci P, Zappacosta F, Fontaine V, Malorni A, Marino G, Ciliberto G. (1991) Single-step purification and structural characterization of human interleukin-6 produced in Escherichia coli from a T7 RNA polymerase expression vector. Eur J Biochem.; 198: 541-7.
• Fujitani Y, Nakajima K, Kojima H, Nakae K, Takeda T, Hirano T. (1994) Transcriptional activation of the IL-6 response element in the junB promoter is mediated by multiple Stat family proteins. Biochem Biophys Res Commun.; 202: 1181-7.
• Gerhartz C, Heesel B, Sasse J, Hemmann U, Landgraf C, Schneider-Mergener J, Horn F, Heinrich PC, Graeve L. (1996) Differential activation of acute phase response factor/STAT3 and STAT1 via the cytoplasmic domain of the interleukin 6 signal transducer gp130. I. Definition of a novel phosphotyrosine motif mediating STAT1 activation. J Biol Chem.; 271: 12991-8.
• Giraud S, Bienvenu F, Avril S, Gascan H, Heery DM, Coqueret O. (2002) Functional interaction of STAT3 transcription factor with the coactivator NcoA/SRC1a. J Biol Chem.; 277: 8004-11.
• Gordon AH, Koj A. (1985) The acute phase response to injury and infection. Elsevier, Amsterdam
• Heinrich PC, Behrmann I, Muller-Newen G, Schaper F, Graeve L. (1998) Interleukin-6-type cytokine signalling through the gp130/Jak/STAT pathway. Biochem J.; 334: 297-314.
• Heinrich PC, Behrmann I, Haan S, Hermanns HM, Muller-Newen G, Schaper F. (2003); Principles of IL-6-type cytokine signalling and its regulation. Biochem J.; 374: 1-20.
• Immenschuh S, Nagae Y, Satoh H, Baumann H, Muller-Eberhard U. (1994) The rat and human hemopexin genes contain an identical interleukin-6 response element that is not a target of CAAT enhancer-binding protein isoforms. J Biol Chem.; 269: 12654-61.
• Immenschuh S, Song DX, Satoh H, Muller-Eberhard U. (1995) The type II hemopexin interleukin-6 response element predominates the transcriptional regulation of the hemopexin acute phase responsiveness. Biochem Biophys Res Commun.; 207: 202-8.
• Kim H, Baumann H. (1997) The carboxyl-terminal region of STAT3 controls gene induction by the mouse haptoglobin promoter. J Biol Chem.; 272: 14571-9.
• May P, Gerhartz C, Heesel B, Welte T, Doppler W, Graeve L, Horn F, Heinrich PC. (1996) Comparative study on the phosphotyrosine motifs of different cytokine receptors involved in STAT5 activation. FEBSLett.; 394: 221-6.
• Oliviero S, Cortese R. (1989) The human haptoglobin gene promoter: interleukin-6-responsive elements interact with a DNA-binding protein induced by interleukin-6. EMBO J.; 8: 1145-51.
• Zhang Z, Fuentes NL, Fuller GM. (1995) Characterization of the IL-6 responsive elements in the gamma fibrinogen gene promoter. J Biol Chem. ; 270: 24287-91.
• Zhang D, Sun M, Samols D, Kushner I. (1996) STAT3 participates in transcriptional activation of the C-reactive protein gene by interleukin-6. J Biol Chem. ; 271: 9503-9.
• Zhang X, Wrzeszczynska MH, Horvath CM, Darnell JE Jr. (1999) Interacting regions in Stat3 and c-Jun that participate in cooperative transcriptional activation. Mol Cell Biol.; 19: 7138-46.