The use amelogenin gene polymorphism in PCR embryo sexing in bovine IVF embryos

• Autorzy: Lechniak D , Cumming J R
• Języki publikacji: EN

Abstrakty

EN

The present study describes a rapid, simple method of bovine IVF embryo sexing by use of PCR technique. A pair of primers corresponding to the bovine amelogenin sequence has been used. The Rapid Cycler (Idaho Technology, USA) used in the current experiment enabled the PCR programme consisting of 55 cycles to be completed in less than 40 minutes. Therefore the total sexing procedure could be performed in less than 90 minutes. The described method succeded in case of 85% analysed embryos.

Słowa kluczowe

EN

polymorphism; sex chromosome; Y chromosome; bovine embryo; sex; amelogenin gene; polymerase chain reaction; embryo

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1997
• Tom: 38
• Numer: 1
• Opis fizyczny: p.45-49,fig.

Twórcy

autor

Lechniak D

• Department of Genetics and Animal Breeding, Agricultural University, Wolynska 33, 60-637 Poznan, Poland

autor

Cumming J R

• Department of Clinical Sciences, Veterinary School, University of Bristol, U.K.

Bibliografia

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