Propozycje dotyczace schematu serologicznej diagnostyki bablowicy w Polsce

• Autorzy: Bitkowska E , Golab E. , Plonka W. , Dzbenski T.H.
• Języki publikacji: PL

Abstrakty

EN

It bas been suggested to change the present way of serological diagnosis of echinococcosis in Poland which utilizes immunoelctrophoresis for confirmation of positive results in a screening test. Diagnostic procedure should be indicated with a sensitive test, such as indirect haemagglutination or immunoenzymatic assay, and every positive result should be verifies later using a Western-blot method in order to detect the most specific reaction with 8 kDa fraction of Echinococcus antigen. Further diferentiation of the infecting species may be accomplished with the aid of Em2 plus ELISA test which detect antibodies speeific for E. multilocularis.

Słowa kluczowe

PL

choroby pasozytnicze; badania serologiczne; parazytologia; diagnostyka; bablowica

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 1999
• Tom: 45
• Numer: 2
• Opis fizyczny: s.229-233,rys.,tab.,bibliogr.

Twórcy

autor

Bitkowska E

• Panstwowy Zaklad Higieny, ul.Chocimska 24, 00-791 Warszawa

autor

Golab E.

autor

Plonka W.

autor

Dzbenski T.H.

Bibliografia

• AYADI A., DUTOIT E., SENDID B., CAMUS D. 1995. Specific diagnostic antigens of Echinococcus granulosus detected by WESTERN-blot. Parasite 2: 119-123.
• BITKOWSKA E., GOŁĄB E., PŁONKA W., DZBEŃSKI T. H. 1997. Zastosowanie metody WESTERN-blot do serologicmej diagnostyki bąblowicy w Polsce. Med. Dośw. Mikrobiol. 49: 215-223.
• GOTTSTEIN B., ECKERT J., MICHAEL S. A., THOMPSON R. C. A. 1987. Echinococcus granulosus antigens: immunoelectrophoretic and Western-blot analysis of hydatid cyst fluids. Parasitol. Res. 73: 186-189.
• LIGHTOWLERS M.W., LIU D., HARALAMBOUS A., RICKARD M. D. 1989. Submit composition and specificity of the major cyst fluid antigens of Echinococcus granulosus. Molec. Biochem. Parasitol. 37: 171-182.
• MADDISONS. E., SLEMENDAS. B., SHANTZ P. M., FRIED J. A., WILSON M., TSANG V. C. 1989. A specific diagnostic antigen of Echinococcus granulosus with an apparent molecular weight of 8 kDA. Am. J. Trop. Med. Hyg. 40: 377-383.
• SHEPHERD J. C., MCMANUS D. P. 1987. Specific and cross-reactive antigens of Echinococcus granulosus hydatid cyst fluid. Molec. Biochem. Parasitol. 25: 143-154.
• VERASTEGUI M., MORO P., GUEVARA A., RODRIGUEZ T., MIRANDA E., GILMAN H. 1992. Enzyme-linked immunoelectrotransfer blot test for diagnosis of human hydatid disease. J. Clin. Mikrobiol. 30: 1557-156.