The mitochondrial localization of RelB and NFATx in immature T cells

• Autorzy: Stasik I , Rapak A. , Ziolo E. , Strzadala L.
• Języki publikacji: EN

Abstrakty

EN

In order to exert their activity, transcription factors must be transported to the nucleus. Certain transcription factors have also been found on mitochondria. Here, the localization of RelB and NFATx in the mitochondrial fractions of normal thymocytes and thymic lymphoma cells is shown for the first time. CREB was only found in the nucleus, while p50 (NFκB) was found in both the nucleus and the cytoplasm, but outside the mitochondria. The translocation of transcription factors to the mitochondria is differentially regulated. Unlike RelB, which is always present in the mitochondrial fraction, NFATx appeared on the mitochondria in cells treated with ionomycin together with an immunosuppressant and inhibitor of calcineurin (FK506). This data reveals that the mitochondrial localization of some transcription factors is precisely controlled by a calcium signal sensitive to FK506 in T cells.

Słowa kluczowe

EN

cell; immature cell; mitochondrial translocation; transcription factor; thymocyte; thymic lymphoma; T cell; activated T cell; nuclear factor; signal transduction

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2008
• Tom: 13
• Numer: 4
• Opis fizyczny: p.493-501,fig.,ref.

Twórcy

autor

Stasik I

• Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland

autor

Rapak A.

autor

Ziolo E.

autor

Strzadala L.

Bibliografia

• 1. Cammarota, M., Paratcha, G., Bevilaqua, L.R., Levi de Stein, M., Lopez, M., Pellegrino de Iraldi, A., Izquierdo, I. and Medina, J.H. Cyclic AMPresponsive element binding protein in brain mitochondria. J. Neurochem. 72 (1999) 2272-2277.
• 2. Yang, S.H., Liu, R., Perez, E.J., Wen, Y., Stevens, S.M., Valencia, T., BrunZinkernagel, A.M., Prokai, L., Will, Y., Dykens, J., Koulen, P. and Simpkins, J.W. Mitochondrial localization of estrogen receptor β. Proc. Natl. Acad. Sci. USA 101 (2004) 4130-4135.
• 3. Ogita, K., Okuda, H., Kitano, M., Fujinami, Y., Ozaki, K. and Yoneda, Y. Localization of activator protein-1 complex with DNA binding activity in mitochondria of murine brain after in vivo treatment with kainite. J. Neurosci. 22 (2002) 2561-2570.
• 4. Cogswell, P.C., Kashatus, D.F., Keifer, J.A., Cuttridge, D.G., Reuther, J.Y., Bristow, C., Roy, S., Nicholson, D.W. and Baldwin, A.S. NFκB and IκBα are found in the mitochondria. J. Biol. Chem. 278 (2003) 2963-2968.
• 5. Li, H., Kolluri, S.K., Gu, J., Dawson, M.I., Cao, X., Hobbs, P.D., Lin, B., Chen, G.Q., Lu, J.S., Lin, F., Xie, Z., Fontana, J.A., Reed, J.C. and Zhang, X.K. Cytochrome c release and apoptosis induced by mitochondrial targeting of nuclear orphan receptor TR3. Science 289 (2000) 1159-1164.
• 6. Sionov, R.V, Cohen, O., Kfir, S., Zilberman, Y. and Yefenof, E. Role of mitochondrial glucocorticoid receptor in glucocorticoid-induced apoptosis. J. Exp. Med. 203 (2006) 189-201.
• 7. Marchenko, N.D., Zaika, A. and Moll, U.M. Death signal-induced localization of p53 protein to mitochondria. J. Biol. Chem. 275 (2000) 16202-16212.
• 8. Barrett, L.E., Van Bockstaele, E.J., Sul, J.Y., Takano, H., Haydon, P.G. and Eberwine, J.H. Elk-1 associates with the mitochondrial permeability transition pore complex in neurons. Proc. Natl. Acad. Sci. USA 103 (2006) 5155-5160.
• 9. Feske, S., Okamura, H., Hogan, P.G. and Rao, A. Ca2+/calcineurin signalling in cells of the immune system. Biochem. Biophys. Res. Commun. 311 (2003) 1117-1132.
• 10. Guerin, S., Baron, M.L., Valero, R., Herrant, M., Auberger, P. and Naquet P. RelB reduces thymocyte apoptosis and regulates terminal thymocyte maturation. Eur. J. Immunol. 32 (2002) 1-9.
• 11. Oukka, M., Ho, I.C., de la Brousse, F.C., Hoey, T., Grusby, M.J. and Glimcher, L.H. The transcription factor NFAT4 is involved in the generation and survival of T cells. Immunity 9 (1998) 295-304.
• 12. Woronicz, J.D., Lina, A., Calnan, B.J., Szychowski, S., Cheng, L. and Winoto, A. Regulation of the Nur77 orphan steroid receptor in activationinduced apoptosis. Mol. Cell Biol. 15 (1995) 6364-6376.
• 13. Matsuda, S., Shibasaki, F., Takehana, K., Mori, H., Nishida, E. and Koyasu, S. Two distinct action mechanisms of immunophilin-ligand complexes for the blockade of T-cell activation. EMBO Rep. 1 (2000) 428-434.
• 14. Woo, J., Ildstad, S.T. and Thomson, A.W. FK506 inhibits the differentiation of developing thymocytes but not negative selection of T cell receptor V beta 5+ and V beta 11+ T lymphocytes in vivo. Transpl. Immunol. 2 (1994) 11-21.
• 15. Kalas, W., Matuszyk, J., Ziolo, E. and Strzadala, L. FK506 restores sensitivity of thymic lymphomas to calcium-mediated apoptosis and the inducible expression of Fas ligand. Anticancer Res. 23 (2B) (2003) 1613- 1617.
• 16. Stasik, I., Rapak, A., Kalas, W., Ziolo, E. and Strzadala, L. Ionomycininduced apoptosis of thymocytes is independent of Nur77 NBRE or NurRE binding, but is accompanied by Nur77 mitochondrial targeting. Biochim. Biophys. Acta. 1773 (2007) 1483-1490.
• 17. Strzadala, L., Miazek, A., Matuszyk, J. and Kisielow, P. Role of thymic selection in the development of thymic lymphomas in TCR transgenic mice. Int. Immunol. 9 (1997) 127-138.
• 18. Bottero, V., Rossi, F., Samson, M., Mari, M., Hofman, P. and Peyron, J.F. IκB-α, the NF-κB inhibitory subunit, interacts with ANT, the mitochondrial ATP/ADP translocator. J. Biol. Chem. 276 (2001) 21317-21324.