Shoot multiplication and plant regeneration of guava [Psidium guajava L.] from nodal explants on in vitro raised plantlets

• Autorzy: Rai M K , Jaiswal V.S. , Jaiswal U.

Warianty tytułu

PL

Rozmnazanie pedow i regeneracja guajawy [Psidium guajava L.] z eksplantatow wezlowych roslin uprawianych in vitro

• Języki publikacji: EN

Abstrakty

EN

A micropropagation method is described for guava (Psidium guajava L.) using nodal expiants from somatic embryo-derived young and aseptic plantlets. Multiple shoots were induced from axillary buds on MS medium containing different concentrations of N6-benzylaminopurine (BAP), either alone or in combination with kinetin (Kn), indole-3-acetic acid (1AA) or a-naphthalene acetic acid (NAA). Medium containing 1 mg l-1 BAP was the most effective for shoot multiplication. In vitro regenerated shoots developed roots either on MS medium alone or on MS medium supplemented with indole-3-butyric acid (1BA). The rooted plantlets were successfully acclimatized.

PL

Opisano metodę mikrorozmnażania guajawy (Psidium guajava L.) używając eksplantatów węzłowych z młodych i aseptycznych roślin otrzymanych na drodze somatycznej embriogenezy. Wiele pędów było inokulowanych z pachwinowych pąków na po żywce MS zawierającej różne stężenia benzyloaminopuryny (BAP), zastosowanej pojedynczo lub w kombinacji z kinetyną (Kn), kwasem indolilooctowym (IAA) albo kwasem a-naftalenooctowym (NAA). Pożywka zawierająca BAP w stężeniu 1 mg l-1 była najbardziej efektywna. Ze zregenerowanych pędów in vitro rozwijały się korzenie zarówno na pożywce MS, jak i na pożywce MS uzupełnionej kwasem indolilomasłowym (IBA). Ukorzenione ro śliny były pomyślnie aklimatyzowane.

Słowa kluczowe

EN

organogenesis; shoot multiplication; in vitro; guava; microshoot; acclimatization; plant regeneration; Psidium guajava; nodal explant; micropropagation; Myrtaceae

• Wydawca: -
• Czasopismo: Journal of Fruit and Ornamental Plant Research
• Rocznik: 2009
• Tom: 17
• Numer: 1
• Opis fizyczny: p.29-38,fig.,ref.

Twórcy

autor

Rai M K

• Banaras Hindu University, Varanasi - 221005, Uttar Pradesh, India

autor

Jaiswal V.S.

autor

Jaiswal U.

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