Evaluation of CASA system for predicting the motility of zebra mussel [Dreissena polymorpha Pallas] spermatozoa

• Autorzy: Bialkowska J. , Dietrich G.J. , Demianowicz W. , Glogowski J.
• Języki publikacji: EN

Abstrakty

EN

A computer – aided semen analyzer (CASA) was used for monitoring some motility parameters of zebra mussel sperm after spawning for up to 1,5h at 22°C and after 24 hours of storage at 8°C. The mean percentage of motility (MOT) for sperm shortly after spawning was 70ą2.0 %, VSL = 31.8ą3.0 µm s-1, and VCL= 110.2ą6.4 µm s-1. The motility expressed as LIN was 27.7ą2.0 %, STR=44.5ą4.8 %, ALH=8.7ą2.0 µm, BCF=6.8ą2.0 Hz. The data demonstrate a consistent decrease in VSL (12.8ą 4.0), VCL (57.4ą 5.7), LIN (15.8ą 3.0) and ALH (1.1ą 0.23) values after 24 hours. No significant differences were found for % of MOT, BCF and STR parameters values during the time of experiment. We found CASA analyzer as a proper tool helpful to discerning the quality of zebra mussel semen samples.

Słowa kluczowe

EN

zebra mussel; Dreissena polymorpha; spermatozoon; motility parameter; sperm; computer aided semen analysis; spawning; motility

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Biology
• Rocznik: 2004
• Tom: 07
• Numer: 2
• Opis fizyczny: http://www.ejpau.media.pl/series/volume7/issue2/biology/art-04.html

Twórcy

autor

Bialkowska J.

• Warmia and Mazury University, Oczapowskiego 5, 10-957 Olsztyn, Poland

autor

Dietrich G.J.

autor

Demianowicz W.

autor

Glogowski J.

Bibliografia

• Bielański W., 1977. Rozród zwierząt. Bydło, owce, konie, świnie [The Animal Reproduction. Cattle, sheep, horses, pigs]. Państwowe Wydawnictwo Rolnicze i Leśne, Warszawa [in Polish].
• Centola G.M., 1996. Comparison of manual microscopic and computer- assisted methods for analysis of sperm count and motility. Arch. Androl. 36: 1-7.
• Christen R., Gatti J.L., Billard R., 1987. Trout sperm motility. The transient movement of trout sperm is related to changes in the concentration of ATP following the activation of the flagellar movement. Eur. J. Biochem., 166(3): 667-671.
• Ciereszko A., Dabrowski, K., Piros, B., Kwasnik, M., Glogowski, J., 2001. Characterization of zebra mussel (Dreissena polymorpha) sperm motility: duration of movement, effects of cations, pH and gossypol. Hydrobiologia, 452: 225-232.
• Davis R., Katz D., 1993. Operational standards for CASA instruments. J. Andrology 14: 385-394.
• Garton D., Haag W., 1993. Seasonal reproductive cycles and settlement patterns of Dreissena polymorpha in western Lake Erie. In T. Nalepa and D. Schloesser (eds.), Zebra Mussel: Biology, Impacts, and Control, Lewis Publishers, Boca Raton, Florida: 111-128.
• Gunzel-Apel A., Gunther P., Bader H., 1993. Computer- assisted analysis of motility, velocity and linearity of dog spermatozoa. J. Reprod. Fertil. Suppl. 47: 271-278.
• Kime D., Ebrahimi M., Nysten K., 1996. Use of computer assisted sperm analysis (CASA) for monitoring the effects of pollution on sperm quality of fish; application to the effects of heavy metals. Aquat. Toxicol. 36: 223-237.
• Kime D., Van Look K., McAllister B., Huyskens G., Rurangwa E., Ollevier F., 2000. Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish. Comp. Biochem. Physiol. Toxicol. Pharmacol. 130 (4): 425-433.
• King L., Holsberger D., Donoghue A., 2000. Correlation of CASA velocity and linearity parameters with sperm mobility phenotype in turkeys. J.Androl. 21(1): 65-71.
• Miller R., Mojares J., Ram, J., 1994. Species – specific sperm attraction in the zebra mussel, Dreissena polymorpha, and quagga mussel, Dreissena bugensis. Can. J. Zool. 72: 1764-1770.
• Mojares J., Stachecki J., Kyozuka K., Armant D., Ram, J., 1995. Characterization of zebra mussel (Dreissena polymorpha) sperm morphology and their motility prior to and after spawning. J. Exp. Zool. 273: 257-263.
• Moore H., Akhondi M., 1996. Fertilizing capacity of rat spermatozoa is correlated with decline in straight –line velocity measured by continuous computer – aided sperm analysis: Epididymal rat spermatozoa from proximal cauda have a greater fertilizing capacity in vitro than those from the distal cauda or has deferens. J. Androl. 17: 50-60.
• Mortimer S., 2000. CASA- Practical Aspects, Andrology Lab Corner. J. Andrology. 21(4): 515-524.
• Oda S., Morisawa M., 1993. Rises of intracellular Ca2+ and pH mediate the initiation of sperm motility by hyperosmomolality in marine teleosts. Cell Mot. Cyt. 25: 171-178.
• Ram J., Crawford G., Walker J., Mojares J., Patel N., Fong P., Kyozuka K., 1993. Spawning in the zebra mussel (Dreissena polymorpha): activation by internal or external application of serotonin. J. Exp. Zool. 265: 587 – 598.
• Ravinder K., Nasaruddin K., Majumdar K., Shivaji S., 1997. Computerized analysis of motility, motility patterns and mitility parameters of spermatozoa of carp following short-term storage of semen. J. Fish Biology. 50, 1309-1328.
• Sprung M., 1987. Ecological requirements of developing Dreissena polymorpha eggs. Arch. Hydrobiol./Suppl. 79: 69-86.
• Stachecki J., Ginsburg K., Armant, D., 1994. Stimulation of cryopreserved epididymal spermatozoa of the domestic cat using the motility stimulants caffeine pentoxifylline and 2’ – deoxyadenosine. J. Androl. 15: 157-164.
• Toth G., Stober J., Read E., Zenick H., Smith M., 1989. The automated analysis of rat sperm motility following subchronic epichlorohydrin administration: methodological and statistical considerations. J. Androl. 10: 401-415.
• Toth G., Stober J., Zenick H., Read E., Christ S., Smith M., 1991. Correlation of sperm motions parameters with fertility in rats treated subchronically with epichlorohydrin. J.Androl. 12: 54-61.
• Toth G., Christ S., McCarthy H., Torsella J., Smith, M., 1995. Computer – assisted motion analysis of sperm from the common carp. J. Fish Biology. 47: 986-1003.
• Vestergen J., Iguer-Ouada M., Onclin K., 2002. Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology. 1: 57(1): 149-179.
• Walker G., Black M., Edwards C., 1995. Comparative morphology of zebra (Dreissena polymorpha) and quagga (Dreissena bugensis) mussel sperm: light and electron microscopy. Can. J. Zool. 74: 809-815.