In vitro and in vivo activity of selected plant crude extracts and fractions against Penicillium italicum

• Autorzy: Kanan G J M , Al-Najar R.A.-W.K.

Warianty tytułu

PL

Aktywnosc in vitro i in vivo wybranych wyciagow roslinnych i ich frakcji przeciwko Penicillium italicum

• Języki publikacji: EN

Abstrakty

EN

The objective of this study is to evaluate (in vitro and in vivo) seven plant extracts and their liquid fractions against four Penicillium italicum isolates. The in vitro study revealed that a concentration 520 μg/ml of crude extract of sticky fleabane leaves or cinnamon bark generated maximum percentage inhibition of 54% and 43%, respectively, against tested fungal isolates. A concentration of 130 μg /ml from each extract (except harmal and garlic where, 390 μg/ml were required) caused complete inhibition of fungal growth of isolates Pi.1 and Pi.3 infecting orange fruit. A concentration of 130 μg/ml of nightshade fruit, fenugreek or sticky fleabane extract inhibited completely the growth of isolates Pi.3 and Pi.5 infecting lemon fruits, whereas a concentration of 390 μg/ml was required to inhibit the growth of isolate Pi.1. Methanolic fractions of cinnamon, garlic or sticky fleabane completely inhibited the growth of fungal isolates. The IC50 values for these fractions were found to be in the range of: 11.2-24; 30.25-31.50; 25.0-36.0 μg/ml, respectively. A concentration 20 μg/ml of cinnamon hexane fraction inhibited the growth of the fungal isolates, with IC50 values of 13, 13.75, 14 and 13 μg/ml, respectively, obtained against isolates Pi.1, Pi.3, Pi.5 and Pi.6. The nightshade hexane fraction completely inhibited the growth of isolates Pi.1 and Pi.3 with IC50 values of 80 and 37.5 μg/ml, respectively. Cinnamon aqueous fraction completely inhibited the growth of isolates Pi.1 and Pi.5 (IC50 were 61.25 and 58.5 μg/ml, respectively).

PL

Oceniano działania (in vitro i in vivo) siedmiu rodzajów wyciągów roślinnych i ich płynnych frakcji przeciwko izolatom Penicillium italicum. Badania in vitro wykazały, że surowy wyciąg z liści Inula viscosa lub kory cynamonu w stężeniu 520 μg/ml, wykazywał maksymalne właściwości inhibicyjne przeciwko badanym izolatom grzyba, które wynosiły odpowiednio 54% i 43%. Każdy z wyciągów użyty w stężeniu 130 μg/ml powodował całkowitą inhibicję wzrostu izolatów grzyba Pi.1 i Pi.3 infekujących owoce pomarańczy (wyjątkiem był Peganum harmala i czosnek, dla których konieczne było stężenie 390 μg/ml). W przypadku wyciągów z owoców Solanum nigrum, Trigonella foenum-graecum i Inula viscosa stężenie 130 μg/ml wywołało całkowitą inhibicję wzrostu izolatów Pi.3 i Pi.5, infekujących owoce cytryny, a do inhibicji wzrostu izolatu Pi.1 potrzebne było stężenie 390 μg/ml. Frakcje metanolowe wyciągów z cynamonu, czosnku i I. viscosa inhibitowały całkowicie wzrost grzybów. Stwierdzone wartości IC50 dla wyżej wspomnianych frakcji wynosiły odpowiednio: 11,2-24; 30,25-31,50; 25,0-36,0 μg/ml. Stężenie 20 μg/ml heksanowej frakcji cynamonu inhibitowało wzrost testowych izolatów, a stwierdzone wartości IC50 przeciwko izolatom Pi.1, Pi.3, Pi.5 i Pi.6 wynosiły odpowiednio 13, 13,75, 14 i 13 μg/ml. Heksanowi frakcja S. nigrum całkowicie inhibitowała wzrost izolatów Pi.1 i Pi.3 (wartości IC50 wynosiły odpowiednio 80 i 37,5 μg/ml). Wodna frakcja cynamonu całkowicie inhibitowała wzrost izolatów Pi.1 i Pi.5 (wartości IC50 wynosiły odpowiednio 61,25 i 58,5 μg/ml).

Słowa kluczowe

PL

plant extract; in vitro activity; in vivo activity; citrus fruit; plant extract fraction; Penicillium italicum; fungi; blue mould; post-harvest disease

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2009
• Tom: 49
• Numer: 4
• Opis fizyczny: p.341-352,fig.,ref.

Twórcy

autor

Kanan G J M

• Mu'tah University, P.O.Box , Karak, Jordan

autor

Al-Najar R.A.-W.K.

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